Bacteriophage therapy for the control of Vibrio harveyi in greenlip abalone (Haliotis laevigata)

Wang, Yanhui; Barton, Mary D.; Elliott, Lisa; Li, Xiaoxu; Abraham, Sam; O’Dea, Mark; Munro, James

doi:10.1016/j.aquaculture.2017.01.003

Cited by 60 publications

(30 citation statements)

References 40 publications

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“…Phage therapy is now being extensively investigated for the application in agriculture (Susianto et al, 2014;Wittmann et al, 2016), and other food industries to eliminate harmful and zoonotic bacteria (Goodridge and Abedon, 2003;Endersen et al, 2014). Research has also focused on the therapeutic potential of phages to treat animal pathogens in both aquatic and terrestrial environments (Wagenaar et al, 2005;Dy et al, 2018;Richards, 2014;Doss et al, 2017;Wang et al, 2017). However, to date, the application of phage therapy in marine turtles has not been attempted.…”

Section: Introductionmentioning

confidence: 99%

Bacteriophage versus antibiotic therapy on gut bacterial communities of juvenile green turtle, Chelonia mydas

Ahasan

Kinobe

Elliott³

et al. 2019

Environmental Microbiology

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Summary Green turtles are endangered marine herbivorous hindgut fermenters that contribute to a variety of marine ecosystems. Debilitated turtles are often rehabilitated in turtle hospitals. Since accurate diagnosis of disease is difficult, broad‐spectrum antibiotics are routinely used as a general treatment, potentially causing collateral damage to the gut microbiome of the patient. Here, we evaluated the concept of the application of bacteriophage (phages) to eliminate targeted intestinal bacteria as an alternative to a broad‐spectrum antibiotic (enrofloxacin) in clinically healthy, captive green turtles. Additionally, the impact of a broad‐spectrum antibiotic (enrofloxacin) and phage therapy on the gut bacterial communities of green turtles was evaluated. Gut bacterial communities in faecal samples were analysed by sequencing the V1‐V3 regions of the bacterial 16S rRNA. Bacteria‐specific phage cocktails significantly (P < 0.05) reduced targeted Acinetobacter in phage‐treated turtles during the therapy. Compared to control, no significant difference was observed in the bacterial diversity and compositions in phage‐treated turtles. In contrast, bacterial diversity was significantly (P < 0.05) reduced in antibiotic‐treated turtles at day 15 and throughout the trial. The alteration in the bacterial microbiota of antibiotic‐treated turtles was largely due to an increase in abundance of Gram‐positive Firmicutes and a concurrent decrease in Gram‐negative Bacteroidetes, Proteobacteria and Verrucomicrobia. Additionally, we observed the relative abundance of several bacteria at lower taxonomic level was much less affected by phages than by antibiotics. These data offer the proof of concept of phage therapy to manipulate transient as well as indigenous bacterial flora in gut‐related dysbiosis of turtles.

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Section: Introductionmentioning

confidence: 99%

Bacteriophage versus antibiotic therapy on gut bacterial communities of juvenile green turtle, Chelonia mydas

Ahasan

Kinobe

Elliott³

et al. 2019

Environmental Microbiology

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“…Currently, research on phage therapy is being revived worldwide due to the crisis of bacterial antimicrobial resistance. Different works have reported the efficiency of phages in the control of pathogenic bacteria both in animals (Wang et al, 2017) and foods (Silva et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Lytic bacteriophages as a potential alternative to control Staphylococcus aureus

Leite¹,

Pereira

Borges

et al. 2019

Pesq. agropec. bras.

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The objective of this work was to characterize autochthonous bacteriophages and to determine their lytic activity on Staphylococcus aureus. Six phages were isolated from dairy barn flush water through enrichment cultures with three S. aureus strains. All phages were characterized by DNA digestion by restriction enzymes and sequencing of the DNA fragment encoding endolysin. Each phage was tested against 100 S. aureus strains isolated from bovine mastitis and from dairy products using the lysis-plate method. The sequences of the endolysin gene were highly conserved, with nucleotide similarity higher than 99% among the isolated phages. Three domains involved in the recognition and lysis of the bacterial cell wall were identified. Two bacteriophages isolated from the dairy barns present high lytic activity on S. aureus, on a wide range of host strains, indicating their potential for studies on phage therapy in dairy cattle or as a biological control agent for dairy products.

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“…Wang et al investigated the use of phage therapy against Vibrio harveyi in abalone infections. It was reported that two Siphoviridae phage isolated from V. harveyi strains significantly improved the survival rate of abalone ( Haliotis laevigata ) and that phage therapy is an effective treatment against vibriosis [32]. Furthermore, Prasad et al used water samples from locations such as fish farms to isolate nine lytic phage against Flavobacterium columnare, which causes cottonmouth disease in fish.…”

Section: Phage Therapy In Marine and Terrestrial Animalsmentioning

confidence: 99%

A Review of Phage Therapy against Bacterial Pathogens of Aquatic and Terrestrial Organisms

Doss

Culbertson

Hahn

et al. 2017

Viruses

213

183

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Since the discovery of bacteriophage in the early 1900s, there have been numerous attempts to exploit their innate ability to kill bacteria. The purpose of this report is to review current findings and new developments in phage therapy with an emphasis on bacterial diseases of marine organisms, humans, and plants. The body of evidence includes data from studies investigating bacteriophage in marine and land environments as modern antimicrobial agents against harmful bacteria. The goal of this paper is to present an overview of the topic of phage therapy, the use of phage-derived protein therapy, and the hosts that bacteriophage are currently being used against, with an emphasis on the uses of bacteriophage against marine, human, animal and plant pathogens.

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Bacteriophage therapy for the control of Vibrio harveyi in greenlip abalone (Haliotis laevigata)

Cited by 60 publications

References 40 publications

Bacteriophage versus antibiotic therapy on gut bacterial communities of juvenile green turtle, Chelonia mydas

Bacteriophage versus antibiotic therapy on gut bacterial communities of juvenile green turtle, Chelonia mydas

Lytic bacteriophages as a potential alternative to control Staphylococcus aureus

A Review of Phage Therapy against Bacterial Pathogens of Aquatic and Terrestrial Organisms

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