New generation polymeric nanospheres for lysozyme adsorption

Abstract

Novel hydrophobic nanospheres with an average size of 100 nm utilizing N-methacryloyl-(l)-tryptophan methyl ester (MAT) as a hydrophobic monomer were prepared by surfactant free emulsion polymerization of 2-hydroxyethyl methacrylate (HEMA) and MAT. MAT was synthesized using methacryloyl chloride and l-tryptophan methyl ester. Specific surface area of the nonporous nanospheres was found to be 1914 m 2 /g. Poly(HEMA-MAT) nanospheres were characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Average particle size, size distribution, and surface charge measurements were also performed. Elemental analysis of MAT for nitrogen was estimated as 1.95 mmol/g polymer. Then, poly (HEMA-MAT) nanospheres were used in the adsorption of lysozyme in batch system. Using an optimized adsorption protocol, a very high loading of 1075 mg lysozyme/ g nanosphere was obtained. The adsorption phenomena appeared to follow a typical Langmuir isotherm. It was observed that enzyme could be repeatedly adsorbed and desorbed without significant loss in adsorption amount or enzyme activity.

Immobilization of lysozyme-cellulose amide-linked conjugates on cellulose I and II cotton nanocrystalline preparations

“…The larger surface area that nano matrices offer creates an ideal substrate property for delivery of bioactive molecules and enhanced enzyme loading. Lysozyme loadings of adsorbed enzyme bound to nanoparticle preparations have recently been reported for polymeric (Akgol et al 2010) and mesoporous (Bhattacharyya et al 2010) nanoparticles from one-half to one gram of enzyme per gram of nanomaterial. On the other hand only one report of a covalent conjugate between cellulose nanocrystals and an enzyme was made in terms of peroxidase activity units (Yang et al 2008).…”

Synthesis and characterization of amino acid containing Cu(II) chelated nanoparticles for lysozyme adsorption

“…The lysozyme adsorption has been widely studied using different matrices to optimize the catalytic features [18][19][20][21][22][23][24][25]. In this study, N-methacryloyl-(L)-tryptophan (MATrp) was purposed as a metalchelating ligand for use in the IMAC of lysozyme.…”

Magnetic Nanoparticles for Plasmid DNA Purification through Hydrophobic Interaction Chromatography

“…Magnetic nanoparticles have been used in separation processes including purification and immunoassays due to their features such as high surface area and magnetic separation properties (46)(47)(48)(49)(50). Hydrophobic interaction chromatography (HIC) is a technique for separation of proteins, peptides, and other biomolecules by their degree of hydrophobicity.…”