New FISH probes – the end in sight

Yung, Jar-Fee

doi:10.1038/ng0996-10

Cited by 8 publications

(4 citation statements)

References 11 publications

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“…Four different types of FISH probe are available (Yung, 1996): centromeric probes, whole chromosome painting probes (WCPPs) or chromosome band-specific painting probes, chromosome-specific unique sequence probes (USPs), and telomeric probes. Centromeric probes contain repetitive DNA sequences found in the centromeric or pericentromeric regions of human chromosomes.…”

Section: Discussionmentioning

confidence: 99%

“…Marker chromosomes account for 0.06-0.12% and 0.07-0.15% of the genetic material in newborn babies and fetuses, respectively, and their origins cannot be ascertained morphologically by G-banding (Sachs et al, 1987). The fluorescence in situ hybridization (FISH) technique is a very important tool for cancer cytogenetic studies, genetic gene mapping, and chromosome disease diagnosis at the DNA level (Fu et al, 1996;Yung, 1996). Since intracytoplasmic sperm injection (ICSI) was first reported in 1992 (Palermo et al, 1992), numerous cases of the highly successful use of ICSI have been reported (Balaban et al, 2001;Ludwig et al, 2001;Madureira et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Identification of the origin of marker chromosomes by two-color fluorescence in situ hybridization and polymerase chain reaction in azoospermic patients

Wei

Yang

et al. 2015

Genet. Mol. Res.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of the origin of marker chromosomes by two-color fluorescence in situ hybridization and polymerase chain reaction in azoospermic patients

Wei

Yang

et al. 2015

Genet. Mol. Res.

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“…These probes can be hybridised to their complementary sequences in metaphase chromosomes fixed to glass slides, and visualised using either radioactive labelling or, nowadays, by fluorescent labels of various colours. Such fluorescence in situ hybridisation, or FISH, [32][33][34] can identify the location of a probed sequence relative to the characteristic banding pattern produced by staining the chromosomes with suitable dyes. If two or more probes, labelled with different fluorophores, are hybridised simultaneously, then their relative positions can be found.…”

Section: Mapping Without Biologymentioning

confidence: 99%

One by one: Single molecule tools for genomics

Dear

2003

Briefings in Functional Genomics and Proteomics

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Much of the effort in any genomics programme arises from the need to generate and purify large numbers of identical molecules, since most analytical tools rely on the analysis of bulk DNA. Biological steps such as bacterial cloning--commonly used to prepare bulk samples of defined DNA fragments--are capricious and introduce their own restrictions and distortions. The analysis of single molecules, either directly or by in vitro enzymatic amplification, makes possible the examination of native genomic DNA without the complications and restrictions of biological propagation. Techniques already exist for the in vitro propagation of genomic fragments and for genome mapping, and offer the advantages of speed, flexibility and predictable behaviour. Single molecule sequencing, for which many approaches are being developed, is more challenging, but offers even greater rewards in terms of throughput and read length.

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“…+ Whole chromosome paints 10 14-16 + Chromosome arm specific paints 17 + Chromosome specific centromeres 18 + + + Satellite III + Chromosome arm specific telomeres [19][20][21][22] + Multicopy, multilocus, sequences + Pan-telomeric + Pan-centromeric + Species specific repeat sequences (for example, Alu) + Unique or low copy sequences + Gene sequences + Non-coding sequences Complementary target sequences for these probes can be recognised on metaphase chromosomes, interphase nuclei from a variety of fresh or archival sources, including paraYnwax embedded tissue sections. 23 24 Clinical applications in cytogenetics PRINCIPLES FISH is generally used either to complement classic staining methods or as a substitute for chromosome identification at metaphase or interphase.…”

Section: Probe Familiesmentioning

confidence: 99%