New findings of Enterocytozoon bieneusi in beef and dairy cattle in Brazil

Fiúza, Vagner Ricardo da Silva; Lopes, Carlos Wilson Gomes; Oliveira, Francisco Carlos Rodrigues de; Fayer, Ronald; Santı́n, Mónica

doi:10.1016/j.vetpar.2015.12.008

Cited by 49 publications

(36 citation statements)

References 40 publications

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“…The present study showed that the infection rate of E. bieneusi in pre-weaned Korean native calves was 16.9%, which is similar to that reported in other studies for cattle in the Republic of Korea (ROK) and several countries [16,25,26,27,28]. The prevalence of E. bieneusi in pre-weaned calves varied from 0 to 50% depending on the farm.…”

Section: Discussionsupporting

confidence: 89%

Zoonotic potential of Enterocytozoon bieneusi in pre-weaned Korean native calves

Hwang

Shin

Kim

et al. 2020

Preprint

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BackgroundEnterocytozoon bieneusi is the most common microsporidian species that can infect humans and various animals worldwide. To date, there has been limited information on the prevalence and genotypes of E. bieneusi infection in cattle in the Republic of Korea. Therefore, this study investigated the prevalence and genotypes of E. bieneusi circulating in pre-weaned Korean native calves and determined the age pattern of E. bieneusi infection and the relationship between E. bieneusi infection and diarrhea. MethodsThe prevalence of E. bieneusi infection in pre-weaned Korean native calves was screened by polymerase chain reaction. PCR-positive products were sequenced to determine the genotype of E. bieneusi. A Chi-square analysis used to compare the associations between diarrhea and infection rate of E. bieneusi in each age ranges or all ages. ResultsPCR and sequencing analysis revealed an overall prevalence of E. bieneusi of 16.9% (53/314) in preweaned calves. The prevalence of E. bieneusi was the highest in September (36.2%), followed by March (28.3%). E. bieneusi infection (χ 2 = 5.82, P = 0.016) was associated with diarrhea in calves. Our results also indicated that E. bieneusi infection was statistically associated with calf age (χ 2 = 11.61, P = 0.003), and the prevalence of E. bieneusi infection was significantly higher in calves aged 21−40 days (odds ratio = 2.90, 95% confidence interval: 1.54−5.45; P = 0.001) than in those aged 1−20 days. Interestingly, the association between E. bieneusi infection and diarrhea was observed only in calves aged 1−20 days (χ 2 = 5.82, P = 0.010). Furthermore, our results indicated that E. bieneusi infection in pre-weaned calves may be associated with mild diarrhea rather than severe diarrhea. We identified five genotypes, BEB4 (n = 12), BEB8 (n = 23), CHN6 (n = 1), I (n = 1), and J (n = 16), and all of which belonged to Group 2. The genotype BEB8 was the most prevalent among all age groups irrespective of diarrhea. In contrast, the genotype I was identified only in one calf aged 10 days with diarrhea. Except for CHN6, the other four genotypes were mostly observed in cattle, and all exhibited zoonotic potential.

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Section: Discussionsupporting

confidence: 89%

Zoonotic potential of Enterocytozoon bieneusi in pre-weaned Korean native calves

Hwang

Shin

Kim

et al. 2020

Preprint

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“…A large variety of animals have been reported to be infected with genotype EbpA. In addition to pigs, EbpA has been detected in non-human primates [37,39], cattle [5,6,11,58], sheep [42], goats [42,59], deer [54], horses [32,45], house mice [36], and birds [15,16], with a wide host range. This genotype has also been found in humans from the Czech Republic [38], Nigeria [2], and China [47], highlighting its zoonotic nature.…”

Section: Genotypesmentioning

confidence: 99%

Molecular detection of Enterocytozoon bieneusi in farm-raised pigs in Hainan Province, China: infection rates, genotype distributions, and zoonotic potential

Zhou

Zheng

et al. 2020

Parasite

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Enterocytozoon bieneusi is a zoonotic fungal pathogen with a high degree of host diversity that can parasitize many animals, including humans. Pigs may play an important role in the epidemiology of E. bieneusi as reservoir hosts. Nevertheless, the genotypes of E. bieneusi in pigs in China remain poorly understood. The aim of this study was to determine the prevalence of E. bieneusi infection amongst pigs raised on farms from four cities of Hainan Province, using nested polymerase chain reaction (PCR) of the partial small subunit of the ribosomal RNA gene, and to identify genotypes of E. bieneusi isolates based on sequence analysis of the ribosomal internal transcribed spacer (ITS) region. Among 188 stool samples, E. bieneusi was detected in 46.8% (88/188). Eight genotypes including four known (EbpA, CS-4, MJ14, and CHG19) and four novel (HNP-I -HNP-IV) genotypes were identified. Using phylogenetic analysis, genotypes EbpA, CS4, CHG19, HNP-III, and HNP-IV were clustered into zoonotic Group 1, while the remaining three genotypes (MJ14, HNP-I, and HNP-II) clustered into Group 10. The high prevalence of zoonotic genotypes of E. bieneusi among pigs suggests that pig farming is a potential source of human infection. Additionally, this is the first identification of genotypes in Group 10 in pigs indicating unique epidemic features of E. bieneusi in pigs in Hainan Province, the southernmost part of China.

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“…It is also lower than that reported in dairy cattle in Argentina (14.3%, 10/70) [16], cattle in USA (34.8%, 285/819) [17], and cattle in Brazil (17.5%, 79/452) [18]. These differences may be influenced by many factors, such as the sample size, animal species, and the region of sample collected, so that it is difficult to explain the real reasons among different studies.…”

Section: Discussionmentioning

confidence: 79%

Detection of Enterocytozoon bieneusi in White Yaks in Gansu Province, China

Zhang

Hou

et al. 2017

BioMed Research International

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Enterocytozoon bieneusi, the most common zoonotic pathogen of microsporidiosis, has been found in various animals and humans, but no information is available concerning the prevalence and genotypes of E. bieneusi in white yaks (Bos grunniens). In the present study, 353 faecal samples from white yaks in Tianzhu Tibetan Autonomous County, Gansu Province, Northwestern China, were collected and examined by PCR amplification of the internal transcribed spacer gene to estimate E. bieneusi prevalence and identify their genotypes. Of the 353 faecal samples, 4 (1.13%) were tested E. bieneusi-positive. Sequences analysis revealed that two known genotypes, namely, I (n = 1) and BEB4 (n = 2), and a novel genotype, namely, WCY1 (n = 1), were found in this study. Among them, genotype WCY1 was clustered into Group 1, and genotypes I and BEB4 belonged to Group 2. The present study firstly indicates the existence of E. bieneusi in yaks in Gansu Province, Northwestern China. This is also the first record of E. bieneusi in white yaks. Effective measures should be taken to control E. bieneusi infection in white yaks, other animals, and humans.

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New findings of Enterocytozoon bieneusi in beef and dairy cattle in Brazil

Cited by 49 publications

References 40 publications

Zoonotic potential of Enterocytozoon bieneusi in pre-weaned Korean native calves

Zoonotic potential of Enterocytozoon bieneusi in pre-weaned Korean native calves

Molecular detection of Enterocytozoon bieneusi in farm-raised pigs in Hainan Province, China: infection rates, genotype distributions, and zoonotic potential

Detection of Enterocytozoon bieneusi in White Yaks in Gansu Province, China

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