New electropolymerizable amphiphilic viologens for the immobilization and electrical wiring of a nitrate reductase

Cosnier, Serge; Galland, Bruno; Innocent, Christophe

doi:10.1016/s0022-0728(97)00207-6

Cited by 68 publications

(41 citation statements)

References 42 publications

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“…This efficient ET may be resulted from the highly flexible siloxane backbone, which has a very small energy barrier to rotation, allows the covalently bound redox mediator into close contact with active center of NiR [30,39], from the highly flexible long chain (13 atoms) spacer, which also allows the covalently linked viologen species to closely contact with active center of NiR. The latter effect has been reported in literature [30,36,40]. Similar electro-enzymatic catalytic reduction of nitrite by NiR and poly viologen co-immobilized electrode was also reported [11].…”

Section: Catalytic Behavior Of Nir and Paps-so 3 H-v Coimmobilized Gcementioning

confidence: 67%

A Nitrite Biosensor Based on Coimmobilization of Nitrite Reductase and Viologen‐Modified Polysiloxane on Glassy Carbon Electrode

2010

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Copper containing nitrite reductase (Cu-NiR) and viologen-modified sulfonated polyaminopropylsiloxane (PAPS-SO 3 H-V) were co-immobilized on glassy carbon electrode (GCE) by hydrophilic polyurethane (HPU) drop-coating, and the electrode was tested as a reagentless electrochemical biosensor for nitrite detection. The newly synthesized PAPS-SO 3 H-V as an electron transfer (ET) mediator between electrode and NiR was effective, and could be effectively immobilized in HPU membrane. The NiR and PAPS-SO 3 H-V co-immobilized GCE used as a nitrite biosensor showed the following performance factors: sensitivity ¼ 12.0 nA mM À1, limit of detection (LOD) ¼ 60 nM (S/N ¼ 3), linear response range ¼ 0 -18 mM (r 2 ¼ 0.996) and response time (t 90% ) ¼ 60 s, respectively. LineweaverBurk plot shows that apparent Michaelis -Menten constant (K app M Þ is 101 mM. Storage stability of the sensor is 51 days (80% of initial activity) in condition of storing in ambient air at room temperature. The sensor showed a relative standard deviation (RSD) of 3.2% (n ¼ 5) even in condition of injection of 1 mM nitrite. Interference study showed that common anions in water sample such as chlorate, chloride, sulfate and sulfite do not interfere with the nitrite detection. However, nitrate interfered with a relative sensitivity of 80% due to inherent character of the enzyme used.

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Section: Catalytic Behavior Of Nir and Paps-so 3 H-v Coimmobilized Gcementioning

confidence: 67%

A Nitrite Biosensor Based on Coimmobilization of Nitrite Reductase and Viologen‐Modified Polysiloxane on Glassy Carbon Electrode

2010

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“…The electrochemical equipment and the purification of Bu 4 NClO 4 (TBAP) have been described previously [24]. Potentials are reported versus the aqueous saturated calomel electrode (SCE).…”

Section: Electrochemical Instrumentation and Proceduresmentioning

confidence: 99%

Electrogeneration of Biotinylated Functionalized Polypyrroles for the Simple Immobilization of Enzymes

et al. 1998

Self Cite

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A new biotin derivative functionalized by a pyrrole group has been synthesized. This functionalization has allowed the preparation of the first example of a poly(pyrrole-biotin) film by oxidative polymerization. In addition, the successful copolymerization of a polypyridinyl complex of ruthenium(II) containing pyrrole groups with the biotin derivative provided redox copolymers exhibiting specific adsorption sites. The resulting copolymer electrodes were applied to the immobilization of a biotinylated glucose oxidase through an avidin bridge. An avidin-conjugated alkaline phosphatase was also immobilized by direct specific adsorption onto the biotinylated polymer. The amperometric response of the resulting biosensors to glucose and phenylphosphate has been studied.

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“…This reaction has been commonly used for amperometric detection of nitrate [6,15,16]. However, as also evident from Equation 1, there is a change in the H þ concentration as the nitrate is reduced to nitrite at the electrode surface.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Redox‐Mediator Supported Potentiometric Nitrate Biosensor with Nitrate Reductase

Sohail

Adeloju

2009

Electroanalysis

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Fabrication of a more superior nitrate potentiometric biosensor than previously achieved with NaR and NADH has been accomplished by co-entrapment of redox mediators and NaR into polypyrrole (PPy) film during galvanostatic polymerization of pyrrole. The replacement of NADH with redox mediators such as thionin acetate (ThAc), safranin (Saf), and azure A (AzA) gave more sensitive potentiometric responses, better minimum detectable concentration, linear concentration range and response time for nitrate than possible with NADH. The co-entrapment of ThAc, Saf, AzA and methyl viologen (MV) with NaR into PPy films also improved the Nernstian behavior of the electrode process beyond the capability of the PPy-NaR-NADH biosensor. Substantial reduction in volume and quantity of cofactor/mediator and, hence cost, was achieved by the replacement of NADH with a redox mediator. Only 50 mM of AzA was required to form a PPy-NaR-AzA biosensor which gave the most sensitive potentiometric response for nitrate, achieving a minimum detectable concentration of 10 mM, a linear concentration range of 50 -5000 mM and a response time of 2 -4 s.

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New electropolymerizable amphiphilic viologens for the immobilization and electrical wiring of a nitrate reductase

Cited by 68 publications

References 42 publications

A Nitrite Biosensor Based on Coimmobilization of Nitrite Reductase and Viologen‐Modified Polysiloxane on Glassy Carbon Electrode

A Nitrite Biosensor Based on Coimmobilization of Nitrite Reductase and Viologen‐Modified Polysiloxane on Glassy Carbon Electrode

Electrogeneration of Biotinylated Functionalized Polypyrroles for the Simple Immobilization of Enzymes

Fabrication of Redox‐Mediator Supported Potentiometric Nitrate Biosensor with Nitrate Reductase

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