New electrochemically-derived plastic antibody on a simple conductive paper support for protein detection: Application to BSA

Ferreira, Nádia; Moreira, Ana; Sá, Maria Helena de; Sales, M. Goreti F.

doi:10.1016/j.snb.2016.12.074

Cited by 24 publications

(11 citation statements)

References 23 publications

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“…Moreover, the potential use of HSA in cancer diagnosis and therapy is highly useful (e.g., neurodegenerative diseases, immune diseases) . HSA is used as the template for protein imprinting, which a key determination in many diseases plays a vital role in maintaining and regulating the osmotic pressure of the blood . Liver, muscles, and kidneys are the main factories for the catabolism of 40–60% of the protein .…”

Section: Introductionmentioning

confidence: 99%

Efficient adsorptive extraction materials by surface protein‐imprinted polymer over silica gel for selective recognition/separation of human serum albumin from urine

Madhumanchi

Jadda

Suedee

2018

J of Applied Polymer Sci

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In this study, we report the development of adsorptive extraction materials by surface protein‐imprinted polymers (MIPs) over silica gel for selective recognition/separation of human serum albumin (HSA) from urine. The HSA‐imprinted polymers prepared on silica particle had at interface between the silica gel and different MIPs greatly produced enrichment for the binding of protein from the urine. The solid‐phase extraction of the optimized polymer layer was prepared by copolymerization of methacrylic acid (MAA), acrylamide (AAm), and dimethylaminoethylmethacrylate (DMAEMA) and a crosslinker methylenebisacrylamide (MBA) at the mole ratio of 1:158:88 (T:M:C) and showed moderate affinity (<104 order M−1) toward target protein HSA and selectivity. Four analogues, egg white albumin (EWA), bovine serum albumin (BSA), lysozyme (Lyz), and creatinine (Cre) were selected to study the binding efficiency of MIPs in single and binary protein solutions. We studied the influence on recognition ability for HSA and found that prepolymer mixture and matrix flexibility of the optimized thin polymer layer (35 ± 10 nm) on the submicrosilica particles. The high‐binding affinity (QMIP, 86.7 mg g−1) and fast kinetics (180 min) were observed for this synthesized HSA‐MIP when compared with other reported HSA‐MIPs in surface imprinting (5.9 and 11.3 mg g−1) and epitope surface imprinting (46.6 mg g−1) methods. We demonstrated the application in real and synthetic urine samples that the approach allowed the efficient adsorption of HSA in real urine (129.48 mg g−1) is almost double to the binding of HSA in synthetic urine (67.84 mg g−1). Apart from this, only minor interference of Cre (2.74 mg g−1) was observed, eventhough Cre is the final metabolite in urine. These adsorptive submicrosilica materials have potential in the pharmaceutical industry and clinical analysis applications. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 46894.

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Section: Introductionmentioning

confidence: 99%

Efficient adsorptive extraction materials by surface protein‐imprinted polymer over silica gel for selective recognition/separation of human serum albumin from urine

Madhumanchi

Jadda

Suedee

2018

J of Applied Polymer Sci

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“…The synthetic urine (s.urine) and healthy human urine solutions were diluted 10 times and used for analytical purposes and spiked with the intended ATP concentrations. Synthetic urine contained urea and creatinine, among other inorganic salts, present in concentrations that followed the literature data [35]. Recent studies have found that the healthy human urine ATP reported about in the range of 0.83-2.7 µM [25].…”

Section: Regeneration and Application To Human Urine Samples Of Adenomentioning

confidence: 62%

Novel adsorptive materials by adenosine 5ʹ‐triphosphate imprinted‐polymer over the surface of polystyrene nanospheres for selective separation of adenosine 5ʹ‐triphosphate biomarker from urine

Jadda

Madhumanchi

Suedee

2019

J of Separation Science

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In this study, we have developed a method to assess adenosine 5ʹ‐triphosphate by adsorptive extraction using surface adenosine 5′‐triphosphate‐imprinted polymer over polystyrene nanoparticles (412 ± 16 nm) for selective recognition/separation from urine. Molecularly imprinted polymer was synthesized by emulsion copolymerization reaction using adenosine 5′‐triphosphate as a template, functional monomers (methacrylic acid, N‐isopropyl acrylamide, and dimethylamino ethylmethacrylate) and a crosslinker, methylenebisacrylamide. The binding capacities of imprinted and non‐imprinted polymers were measured using high‐performance liquid chromatography with UV detection with a detection limit of 1.6 ± 0.02 µM of adenosine 5′‐triphosphate in the urine. High binding affinity (QMIP, 42.65 µmol/g), and high selectivity and specificity to adenosine 5′‐triphosphate compared to other competitive nucleotides including adenosine 5ʹ‐diphosphate, adenosine 5ʹ‐monophosphate, and analogs such as adenosine, adenine, uridine, uric acid, and creatinine were observed. The imprinting efficiency of imprinted polymer is 2.11 for urine (QMIP, 100.3 µmol/g) and 2.51 for synthetic urine (QMIP, 48.5 µmol/g). The extraction protocol was successfully applied to the direct extraction of adenosine 5′‐triphosphate from spiked human urine indicating that this synthesized molecularly imprinted polymer allowed adenosine 5′‐triphosphate to be preconcentrated while simultaneously interfering compounds were removed from the matrix. These submicron imprinted polymers over nano polystyrene spheres have a potential in the pharmaceutical industries and clinical analysis applications.

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“…A positively charged monomer, quaternary ammonium salt, holding a vinyl bond and an aromatic ring (VBTC), was used to assemble the MIPs for bovine serum albumin (BSA) which holds a negative charge under analytical conditions (pH 7.4, isoelectric point is 5.4). It promoted the ionic interaction between BSA and the MIPs [22]. π-π interaction was used to recognize toxic protein aflatoxin B1 by the p-aminothiophenol-based MIPs.…”

Section: Toxins and Other Protein Biomarkersmentioning

confidence: 99%

Molecularly Imprinted Polymers and Surface Imprinted Polymers Based Electrochemical Biosensor for Infectious Diseases

Cui

Zhou

2020

Sensors

147

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Owing to their merits of simple, fast, sensitive, and low cost, electrochemical biosensors have been widely used for the diagnosis of infectious diseases. As a critical element, the receptor determines the selectivity, stability, and accuracy of the electrochemical biosensors. Molecularly imprinted polymers (MIPs) and surface imprinted polymers (SIPs) have great potential to be robust artificial receptors. Therefore, extensive studies have been reported to develop MIPs/SIPs for the detection of infectious diseases with high selectivity and reliability. In this review, we discuss mechanisms of recognition events between imprinted polymers with different biomarkers, such as signaling molecules, microbial toxins, viruses, and bacterial and fungal cells. Then, various preparation methods of MIPs/SIPs for electrochemical biosensors are summarized. Especially, the methods of electropolymerization and micro-contact imprinting are emphasized. Furthermore, applications of MIPs/SIPs based electrochemical biosensors for infectious disease detection are highlighted. At last, challenges and perspectives are discussed.

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New electrochemically-derived plastic antibody on a simple conductive paper support for protein detection: Application to BSA

Cited by 24 publications

References 23 publications

Efficient adsorptive extraction materials by surface protein‐imprinted polymer over silica gel for selective recognition/separation of human serum albumin from urine

Efficient adsorptive extraction materials by surface protein‐imprinted polymer over silica gel for selective recognition/separation of human serum albumin from urine

Novel adsorptive materials by adenosine 5ʹ‐triphosphate imprinted‐polymer over the surface of polystyrene nanospheres for selective separation of adenosine 5ʹ‐triphosphate biomarker from urine

Molecularly Imprinted Polymers and Surface Imprinted Polymers Based Electrochemical Biosensor for Infectious Diseases

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