New efficient producers of fungal laccases

Myasoedova, N. M.; Chernykh, A.M.; Psurtseva, Nadezhda V.; Belova, N. V.; Golovleva, L. A.

doi:10.1134/s0003683808010122

Cited by 32 publications

(27 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…However, laccase production by T. versicolor doubled when veratryl alcohol or guaiacol was used instead of 2,5-xylidine [11]. The best inducer for the Lentinus strigosus laccase synthesis is reported to be 2,6-dimethoxyphenol elevating the enzyme yield 8-fold compared to the control, whereas veratryl alcohol was ineYcient for the cultures studied or induced the enzyme production insigniWcantly [13]. This means that the fungusspeciWc inducer should be found in order to maximally express the target enzyme secretion.…”

Section: Introductionmentioning

confidence: 98%

Effect of aromatic compounds on the production of laccase and manganese peroxidase by white-rot basidiomycetes

Elisashvili¹,

Kachlishvili²,

Khardziani³

et al. 2010

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

Three white-rot fungi displayed a wide diversity in their response to supplemented aromatic compounds. Pyrogallol stimulated Cerrena unicolor laccase and manganese peroxidase (MnP) synthesis in synthetic medium 2.5- and 2-fold, respectively, whereas 2,4,6-trinitrotoluene (TNT) brought about a 2.8-fold increase in laccase yield by Trametes versicolor in submerged fermentation of ethanol production residue. No effect of the tested aromatic compounds on enzyme secretion by Ganoderma lucidum in mannitol-containing medium was detected. Nevertheless, G. lucidum is a potent producer of laccase in submerged fermentation of wheat bran and enzyme synthesis can be further increased by supplementation of medium with an appropriate inducer. The structure and the concentration of aromatic compounds play an important role in the regulation of enzyme synthesis. The supplementation of synthetic medium with 0.03-0.3 mM TNT or hydroquinone increased the differential rate of laccase synthesis by C. unicolor from 1,267 to 3,125-8,630 U mg biomass(-1) day(-1). Moreover, the same aromatic compound may function as either an inducer or a repressor, depending on the fungus and enzyme studied. Thus, hydroquinone increased 3-fold T. versicolor laccase activity decreasing 2- and 8-fold the yields of MnP and endoglucanase, respectively.

show abstract

Section: Introductionmentioning

confidence: 98%

Effect of aromatic compounds on the production of laccase and manganese peroxidase by white-rot basidiomycetes

Elisashvili¹,

Kachlishvili²,

Khardziani³

et al. 2010

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…In view of the broad biotechnological applications of laccases [16][17][18], there is a scientific need to identify different sources of laccases having diverse properties so that suitable laccases for various applications could be identified.…”

Section: +mentioning

confidence: 99%

Properties of crude laccase from Trametes versicolor produced by solid-substrate fermentation

Стоилова¹,

Krastanov²,

Stanchev³

2010

ABB

112

View full text Add to dashboard Cite

A low-cost process for the production of laccases is necessary for a sustainable enzymatic wastewater treatment. Therefore, it is necessary to establish an easy and low-cost procedure for the production of laccase. In the present study the properties of crude laccase from Trametes versicolor produced by solidsubstrate fermentation is investigated. The application of the enzyme for dye decolorization is also studied. Crude laccase from the studied culture established maximal activity at 45ºC. The enzyme retained over 90% of its activity in the temperature range 40-47ºC and pH 4.5. The kinetic constants of the crude enzyme was also determined. In the presence of KCl, NaCl, CaCl 2 , MnSO 4 and MgSO 4 , laccase demonstrated high stability-over 50% of its initial activity was still retained after 4-month incubation.

show abstract

“…Also, there are many studies engaged with the type, the time of addition inducers and the concentration of the inducers to enhance the lacasse production. Most of the used inducers are phenolic or aromatic compounds related to lignin or lignin derivates (Klonowska et al 2001;Myasoedova et al 2008). (200801) were examined to investigate their effects on the lacasse production under a variety of culture conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Myasoedova et al (2008) studied with L. strigosus by inserting inducer such as CuSO4 and 2,6-dimethylphenol and found 186.5 U ml -1 . This high lacasse activity may result from localization of isolation and type of inducers used.…”

Section: In This Study 20 Basidiomycetes Isolates Such As P Arcularmentioning

confidence: 99%

Enhancement with inducers of lacasse production by some strains and application of enzyme to dechlorination of 2,4,5-trichlorophenol

Gedikli¹,

Aytar²,

Ünal³

et al. 2010

Electron Journ of Biotechnol

View full text Add to dashboard Cite

Lacasse is one of the extracellular enzymes excreted from white and brown rot fungi, which is involved in ligninolysis. In the present study, the effects of the addition of lacasse inducers to the medium on enhancement of enzyme production under conditions of submerged fermentation were researched. At first, a culture medium was selected suitable for lacasse production. To increase the production of lacasse using different inducers and to examine the ability of dechlorination, this article focuses on screen lacasse activity of 21 basidiomycetes isolates grown in five culture media. All inducers evaluated influenced lacasse activity positively except for gallic acid, mannitol, and malt extract for studied isolates. Our findings showed that lacasse activity of Trametes versicolor ATCC (200801) when it was induced with wheat bran reached up to 29.08 U ml -1 and was examined the ability of dechlorination of 2,4,5-trichlorophenol (2,4,5-TCP). The parameters including pH, initial substrate concentration, amount of enzyme, period of reaction, and temperature were tested for dechlorination process. Correlation between oxygen consumption and dechlorination processes under the determined optimum conditions was analyzed. Toxicity of 2,4,5-TCP before and after enzymatic treatment was evaluated by Microtox test. The results demonstrated that toxicity of intermediates formed 2,4,5-TCP did not change.

show abstract

New efficient producers of fungal laccases

Cited by 32 publications

References 21 publications

Effect of aromatic compounds on the production of laccase and manganese peroxidase by white-rot basidiomycetes

Effect of aromatic compounds on the production of laccase and manganese peroxidase by white-rot basidiomycetes

Properties of crude laccase from Trametes versicolor produced by solid-substrate fermentation

Enhancement with inducers of lacasse production by some strains and application of enzyme to dechlorination of 2,4,5-trichlorophenol

Contact Info

Product

Resources

About