Properties of crude laccase from Trametes versicolor produced by solid-substrate fermentation

Стоилова, Иванка; Krastanov, Albert; Stanchev, Veselin

doi:10.4236/abb.2010.13029

Cited by 110 publications

(53 citation statements)

References 24 publications

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“…The pH profile for Trametes versicolor laccase is a bell shaped curve with optimum activity at 4.5 and decreasing gradually as the pH increases [32]. This might explain the better performance of the pH control system over the buffer strength and CEM systems.…”

Section: Enzyme Activitymentioning

confidence: 98%

Decolourisation of Acid orange 7 in a microbial fuel cell with a laccase-based biocathode: Influence of mitigating pH changes in the cathode chamber

Mani

Keshavarz

Chandra

et al. 2017

Enzyme and Microbial Technology

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Highlights of Research1. First Study to observe the influence of mitigating pH changes and its effect on laccase activity in a microbial fuel cell (MFC).2. Decoupling pH and salinity in the cathode chamber of MFC.3. Correlation of laccase activity with performance of the system. Results show that using Nafion 117 membrane limits salinity and pH changes in the cathode (100 mM acetate buffer as catholyte) leading to prolonged laccase activity and faster AO7 decolourisation compared to using CMI7000 as a membrane; similarly automatic pH control in the cathode chamber was found to be better than using 200 mM acetate buffer.It is suggested that while pH control in the cathode chamber is important, it does not guarantee sustained laccase activity; that salinity increases affect the activity and could be mitigated using a cation selective membrane.

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Section: Enzyme Activitymentioning

confidence: 98%

Decolourisation of Acid orange 7 in a microbial fuel cell with a laccase-based biocathode: Influence of mitigating pH changes in the cathode chamber

Mani

Keshavarz

Chandra

et al. 2017

Enzyme and Microbial Technology

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“…La función del CaCl 2 en el medio de cultivo es mantener la estructura y estabilidad de ambas enzimas. Del mismo modo, Stoilova et al (2010) sugieren que concentraciones bajas de MgSO 4 contribuyen a mantener la estabilidad de la lacasa durante periodos prolongados de incubación. Sin embargo, las altas concentraciones de sales ((NH 4 ) 2 SO 4 , CaCl 2 , MgSO 4 y KH 2 PO 4 ) en el medio de cultivo incrementan su potencial osmótico y en consecuencia, se puede reducir el crecimiento del micelio (Beever y Laracy 1986).…”

Section: Discussionunclassified

Aislamiento Y Evaluación De La Actividad Enzimática Ligninolítica De Macromicetos Del Estado De Veracruz, México

Chan-Cupul

Abarca

Vázquez

2016

Rev. Int. Contam. Ambie.

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Palabras clave: biotecnología ambiental, lacasa, manganeso peroxidasa, micobiota nativa, fermentación líquida y sólida RESUMENLa diversidad fúngica del estado de Veracruz, México, es una importante fuente para la obtención de enzimas con valor biotecnológico. El objetivo de este estudio fue aislar y evaluar cualitativa (placas de agar) y cuantitativamente (en fermentación líquida y sólida) la actividad enzimática ligninolítica de macromicetos nativos. De un total de 70 aislamientos, 41 presentaron actividad lacasa en la evaluación cualitativa. Por destacar en el ensayo cualitativo se seleccionaron las cepas Trametes maxima SM9, Pycnoporus sanguineus ACT1 y Daedalea elegans PM7 para la evaluación cuantitativa de la actividad lacasa y manganeso peroxidasa (MnP) en los medios de cultivo líquido: Bose, Koroljova y Sivakumar, así como en los residuos agroindustriales: paja de trigo, bagazo de caña y aserrín de pino. En el medio Sivakumar se detectó la mayor actividad lacasa y MnP. Trametes maxima SM9 presentó los valores más altos para lacasa (9121.8 U/mg de proteína) y MnP (477.9 U/mg de proteína), en segundo término estuvo P. sanguineus ACT1 (lacasa = 5422.2 U/mg de proteína y MnP = 41.0 U/mg de proteína) y Daedalea elegans PM7 (lacasa = 1784.2 U/mg de proteína y MnP = 40.0 U/mg de proteína). En fermentación sólida P. sanguineus ACT1 obtuvo la mayor actividad lacasa en paja de trigo (1409.0 U/mg de peso seco) y bagazo de caña (1404.8 U/mg de peso seco). Mientras que la actividad MnP fue mejor sobre bagazo de caña tanto para P. sanguineus ACT1 y T. maxima SM9 con 619.0 y 519.6 U/mg de peso seco, respectivamente. Las cepas Trametes maxima SM9 y P. sanguineus ACT1 son consideradas como buenas candidatas para la producción de lacasa y MnP.

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“…Every value represents the mean of triplicate measurements that varied from the mean by no more than 8 % laccase exists as protein [14], these can affect the spatial conformation of laccase. Regarding conformational changes of laccase, these are highly dependent on the state of oxidation of the copper atoms in active center [16]. So the enzyme activity in anode will be best.…”

Section: The Effect Of Electric Field On the Concentration Of Laccasementioning

confidence: 99%

Effect of Direct-Current Electric Field on Enzymatic Activity and the Concentration of Laccase

et al. 2015

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This work investigates the effect of directcurrent electric field on the extracellular enzymatic activity, concentration and other experimental parameters of laccase from Trametes versicolor. The results showed that laccase could significantly contribute to the change of pH at the end of graphite electrode. In addition, it increased the electrical conductivity of the water. In the experiment, the optimum pH and catalytic pH range for laccase activity were 3.0 and pH 2.5-4.0. The application of 6 V direct current showed significant effects on the laccase enzyme activity. The activity of laccase was enhanced in the anodic region, but at the same time was strongly inhibited at the cathode. The electric charge characteristics of laccase were changed when exposed to electric field, and some laccases molecules moved to the anode, which produced a slight migration phenomenon. This study is the basis of combination of laccase and electrical technology, at the same time, providing a new direction of enhancing laccase activity. Compared to immobilization, using electric field is simple, no chemical additives, and great potential.

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Properties of crude laccase from Trametes versicolor produced by solid-substrate fermentation

Cited by 110 publications

References 24 publications

Decolourisation of Acid orange 7 in a microbial fuel cell with a laccase-based biocathode: Influence of mitigating pH changes in the cathode chamber

Decolourisation of Acid orange 7 in a microbial fuel cell with a laccase-based biocathode: Influence of mitigating pH changes in the cathode chamber

Aislamiento Y Evaluación De La Actividad Enzimática Ligninolítica De Macromicetos Del Estado De Veracruz, México

Effect of Direct-Current Electric Field on Enzymatic Activity and the Concentration of Laccase

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