New directions of miniaturization within the proteomics research area

Markó-Varga, György; Nilsson, Johan; Laurell, Thomas

doi:10.1002/elps.200305666

Cited by 35 publications

(29 citation statements)

References 54 publications

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“…However, more easily automated LC techniques, often combined with MS, have begun to replace 2-D PAGE [26,27]. Gelfree proteomics can also more easily take advantage of labeling of the samples with stable isotopes [28] or chipbased techniques [29,30].…”

Section: Proteomic Techniques Available For Musculoskeletal Researchmentioning

confidence: 99%

Proteomic analysis of cartilage‐ and bone‐associated samples

2006

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The skeleton of the human body is built of cartilage and bone, which are tissues that contain extensive amounts of extracellular matrix (ECM). In bone, inorganic mineral hydroxyapatite forms 50-70% of the whole weight of the tissue. Although the organic matrix of bone consists of numerous proteins, 90% of it is composed of type I collagen. In cartilage, ECM forms a major fraction of the tissue, type II collagen and aggrecans being the most abundant macromolecules. It is obvious that the high content of ECM components causes analytical problems in the proteomic analysis of cartilage and bone, analogous to those in the analysis of low-abundance proteins present in serum. The massive contents of carbohydrates present in cartilage proteoglycans, and hydroxyapatite in bone, further complicate the situation. However, the development of proteomic tools makes them more and more tempting also for research of musculoskeletal tissues. Application of proteomic techniques to the research of chondrocytes, osteoblasts, osteocytes, and osteoclasts in cell cultures can immediately benefit from the present knowledge. Here we make an overview to previous proteomic research of cartilage- and bone-associated samples and evaluate the future prospects of applying proteomic techniques to investigate key events, such as cellular signal transduction, in cartilage- and bone-derived cells.

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Section: Proteomic Techniques Available For Musculoskeletal Researchmentioning

confidence: 99%

Proteomic analysis of cartilage‐ and bone‐associated samples

2006

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“…Recently, we presented alternatives of microchip configuration and geometries, and their respective performance in terms of operational stability, sensitivity and microfluidic characteristics [53][54][55][56][57][58]. In the new development, the sample loading and washing was separated and performed off-line from the actual extraction and sample spotting onto the MALDI target.…”

Section: Sample Processing Integrated With Sample Spottingmentioning

confidence: 99%

New directions of miniaturization within the biomarker research area

2004

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An overview of the current trends within protein expression profiling is given where multidimensional separation of both gel and liquid phase techniques linked to mass spectrometry is viewed as a major route in the global proteome mapping. A clear trend in these biochemical developments is the effort to sequence and identify low-abundant protein expressions where assay miniaturization and integrated sample processing play a central role. Two areas of miniaturization within the proteomics field are addressed: (i) sample cleanup and enrichment, and (ii) silicon microstructure developments for protein chip microarrays.

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“…Different configurations such as single and triple quadrupole, TOF, IT, and ultrahigh-resolution Fouriertransform ion cyclotron resonance (FTICR) mass spectrometers were adapted to chip-based ESI and contributed significant benefits for various studies. However, as nicely illustrated already by a number of reviews [8,11,[20][21][22] the general areas of implementation and applications of chip technologies either silicon-based, glass, or polymer microchips have by far been primarily genomics, drug discovery, and proteomics.…”

Section: Introductionmentioning

confidence: 99%

Chip electrospray mass spectrometry for carbohydrate analysis

Zamfir¹,

Bîndilă²,

Lion³

et al. 2005

Electrophoresis

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Currently two types of chip systems are used in conjunction with MS: out-of-plane devices, where hundreds of nozzles, nanospray emitters are integrated onto a single silicon substrate from which electrospray is established perpendicular to the substrate, and planar microchips, embedding a microchannel at the end of which electrospray is generated in-plane, on the edge of the microchip. In the last two years, carbohydrate research greatly benefited from the introduction and implementation of the chip-based MS. In two laboratories the advantages of the chip electrospray in terms of ionization efficiency, sensitivity, reproducibility, quality of data in combination with high mass accuracy, and resolution of detection were systematically explored for several carbohydrate classes: O-and N-glycopeptides, oligosaccharides, gangliosides and glycoprotein-derived O-and N-glycans, and glycopeptides. The current state-of-the-art in interfacing the chip electrospray devices to high-performance MS for carbohydrate analysis, and the particular requirements for method optimization in both positive and negative ion modes are reviewed here. The recent applications of these miniaturized devices and their general potential for glycomic-based surveys are highlighted.

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New directions of miniaturization within the proteomics research area

Cited by 35 publications

References 54 publications

Proteomic analysis of cartilage‐ and bone‐associated samples

Proteomic analysis of cartilage‐ and bone‐associated samples

New directions of miniaturization within the biomarker research area

Chip electrospray mass spectrometry for carbohydrate analysis

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