New diagnostic markers in basal cell carcinoma

High‐throughput lipidomic profiles sampled with electroporation‐based biopsy differentiate healthy skin, cutaneous squamous cell carcinoma, and basal cell carcinoma

“…Previous studies comparing BCC and healthy (with sample sizes of 30 and 64) 21,23 and a study comparing 12 BCC, 13 AK, and 11 healthy skin samples, 22 reported lipidomic profiles for only six lipid groups: cholesterol, HDL, LDL, triglycerides, phospholipids, and total lipids. Triglycerides were previously reported as significantly higher in BCC versus Healthy skin samples, 22 but not significant in serum samples for the same comparison 21–23 . Phospholipids were previously found significantly higher in BCC versus Healthy in both skin and serum samples 22 …”

“…To the best of our knowledge, no previous molecular profiling study focused only on the lipidomic profiling of cSCC. Previous studies comparing BCC and healthy (with sample sizes of 30 and 64) 21,23 and a study comparing 12 BCC, 13 AK, and 11 healthy skin samples, 22 reported lipidomic profiles for only six lipid groups: cholesterol, HDL, LDL, triglycerides, phospholipids, and total lipids. Triglycerides were previously reported as significantly higher in BCC versus Healthy skin samples, 22 but not significant in serum samples for the same comparison.…”

“…Triglycerides were previously reported as significantly higher in BCC versus Healthy skin samples, 22 but not significant in serum samples for the same comparison. [21][22][23] Phospholipids were previously found significantly higher in BCC versus Healthy in both skin and serum samples. 22 Our study significantly improves on the level of detail of reported lipids of BCC and healthy skin.…”

“…First, the sample size was not representative enough to draw a confident conclusion on the specific lipid behavior. Previous studies demonstrate the need for larger sample numbers to observe trends in skin cancer lipids, 21 , 23 thus an increased sample size can improve differential expression analysis, increase the confidence in findings, and reduce the amount of falsely detected signals. Second, the study was performed on the ex‐vivo samples, in‐vivo sampling of skin may provide slightly different results.…”

“… 13 , 14 , 15 Previously, high‐throughput analyses of genes, proteins, and metabolites have been reported for cSCC and BCC. 16 , 17 , 18 , 19 , 20 Lipids profiles, however, have mostly been reported for BCC 21 , 22 , 23 using low‐throughput methods and serum samples with the exception of one high‐throughput cSCC study that included 70 lipids among metabolites. 24 Beyond medicine, molecular profiling plays an important role in other aspects of life such use in agriculture food and safety, forensic science, and environmental monitoring.…”