New developments in solid state fermentation: I-bioprocesses and products

Pandey, Ashok; Soccol, Carlos Ricardo; Mitchell, David A.

doi:10.1016/s0032-9592(00)00152-7

Cited by 934 publications

(521 citation statements)

References 126 publications

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“…Maximum enzyme production could be obtained only after a certain incubation period which allows the culture to grow at a steady state (Pandey et al, 2000). Five days was the ideal incubation period, giving L-asparaginase activity of 192.3 U/ml (Table 3).…”

Section: Effect Of Different Incubation Periodsmentioning

confidence: 99%

Production of Extracellular Anti-leukemic Enzyme L-asparaginase from Fusarium solani AUMC 8615 Grown under Solid-State Fermentation conditions: Purification and Characterization of The Free and Immobilized Enzyme

2016

Egypt. J. Bot.

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OTENTIALITIES of twenty four fungal isolates were investigated for their ability to produce L-asparaginase using modified Czapek's Dox medium. Fusarium solani AUMC 8615 was selected as the most potent fungal strain for the enzyme production under solid state fermentation (SSF) using agro-industrial residues. Wheat bran supported maximum enzyme production followed by rice bran and corn cob. The optimum conditions for maximum production of L-asparaginase under SSF occurred on the fifth day of incubation at 30°C, pH 8.0, 60% of initial moisture content and supplementation with 0.2% (w/v) glucose and 0.5% (w/v) ammonium sulphate. Lasparaginase was purified to homogeneity by ammonium sulphate precipitation, gel filtration and ion exchange chromatography giving 299.58 purification fold. SDS-PAGE showed that the purified enzyme consists of two subunits of molecular weights 70 and 80 kDa, respectively. The enzyme was efficiently immobilized by covalent binding with activated charcoal giving an immobilization yield of 80.40%. Optimum pH values were 9.0 and 8.0 for free and immobilized enzyme, respectively. While, Optimum reaction temperatures were 37°C and 45°C for free and immobilized enzyme, respectively. After incubation for 2 hr at 37°C, the relative activity of free enzyme decreases to 35% whereas for the immobilized enzyme decreased only to 57% at 45°C.

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Section: Effect Of Different Incubation Periodsmentioning

confidence: 99%

Production of Extracellular Anti-leukemic Enzyme L-asparaginase from Fusarium solani AUMC 8615 Grown under Solid-State Fermentation conditions: Purification and Characterization of The Free and Immobilized Enzyme

2016

Egypt. J. Bot.

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“…Among these residues, sugarcane bagasse is one of the most important in Brazil and is composed by approximately 50% cellulose, 25% hemicellulose and 20% lignin (PANDEY et al, 2000). Wheat bran is also a mostly available waste which comprises about 46% of polysaccharides (glucuronoarabinoxylans, cellulose, mixed linked (1-3)(1-4)-β-D-glucans and starch), proteins (15-22%) and lignin (4-8%) (SOUZA et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes

Gomes¹,

Santos²,

Franciscon³

et al. 2016

Biosci. J.

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This study reports the optimization of xylanase production under solid state fermentation (SSF) by a thermotolerant Aspergillus fumigatus strain (SCB4) isolated from sugarcane bagasse piles of Brazilian Cerrado. Different combinations of low-cost agricultural byproducts in SSF were evaluated: sugarcane bagasse and wheat bran (1:1), sugarcane bagasse and corn straw (1:1) and only sugarcane bagasse. The enzyme biosynthesis by SSF was carried out at different temperatures (40, 45, 50 and 55 o C). The maximum levels of xylanase activity were obtained after 24 h at 45 °C using a culture medium containing sugarcane bagasse and wheat bran (1:1). Under optimal conditions, the fungal culture produced 574 U g -1 of xylanase (units/g of dry substrate). The crude enzyme showed optimal activity at 60 °C and pH 4.5. It exhibited thermostability up to 55 °C, wide range of pH stability and tolerance to ethanol, xylose and glucose. The physicochemical properties shown by this enzyme are appropriate for its application in hydrolysis of lignocellulosic residues for ethanol production and other bioproducts.

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“…One unit (U) of exo-chitinase activity is defined as the amount of enzyme that is required to release 1 lmol of N-acetyl-b-Dglucosamine per minute from 0.5% of dry colloidal chitin solution under standard bioassay conditions and expressed as Units per gram dry substrate (U gds -1 ) [8].…”

Section: Estimation Of Exo-chitinase Activitymentioning

confidence: 99%

“…One variable was varied at a time keeping other variable constant, it included the incubation time (24-120 h), incubation temperature (20-45°C), pH (4)(5)(6)(7)(8)(9)(10)(11)(12), salt concentration (2-6% NaCl), effect of nitrogen sources such as peptone, yeast extract, urea, ammonium chloride, ammonium sulphate, casein and various carbon sources such as arabinose, fructose, D-glucose, mannose, starch, xylose. For each parameter optimization, three sets of independent experiments were carried out and the average values were reported.…”

Section: Estimation Of Exo-chitinase Activitymentioning

confidence: 99%

Studies on Exo-Chitinase Production from Trichoderma asperellum UTP-16 and Its Characterization

et al. 2011

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The growth conditions for chitinase production by Trichoderma asperellum UTP-16 in solid state fermentation was optimized using response surface methodology based on central composite design. The chitinase production was optimized, using one-factor at a time approach, with six independent variables (temperature, pH, NaCl, incubation period, nitrogen and carbon sources) and 3.31 Units per gram dry substrate (U gds -1 ) exo-chitinase yield was obtained. A 21.15% increase was recorded in chitinase activity (4.01 U gds -1 ) through surface response methodology, indicates that it is a powerful and rapid tool for optimization of physical and nutritional variables. Further, efficiency of crude enzyme was evaluated against phytopathogenic Fusarium spp. and a mycelial growth inhibition up to 3.5-6.5 mm was achieved in well diffusion assay. These results could be supplemented as basic information for the development of enzyme based formulation of T. asperellum UTP-16 and its use as a biocontrol agent.

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New developments in solid state fermentation: I-bioprocesses and products

Cited by 934 publications

References 126 publications

Production of Extracellular Anti-leukemic Enzyme L-asparaginase from Fusarium solani AUMC 8615 Grown under Solid-State Fermentation conditions: Purification and Characterization of The Free and Immobilized Enzyme

Production of Extracellular Anti-leukemic Enzyme L-asparaginase from Fusarium solani AUMC 8615 Grown under Solid-State Fermentation conditions: Purification and Characterization of The Free and Immobilized Enzyme

Substract and temperature effect on xylanase production by aspergillus fumigatus using low cost agricultural wastes

Studies on Exo-Chitinase Production from Trichoderma asperellum UTP-16 and Its Characterization

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