New data on the occurrence of ochratoxin A in human sera from patients affected or not by renal diseases in Tunisia

Grosso, F.; Saïd, Salem; Mabrouk, Imed; Frémy, J. M.; Castegnaro, M.; Jemmali, M.; Dragacci, Sylviane

doi:10.1016/s0278-6915(03)00067-x

Cited by 79 publications

(40 citation statements)

References 26 publications

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“…OTA is nephrotoxic and is a potent renal carcinogen in rats (7) and in chicks (8). In humans, OTA has been associated with nephropathies in the Balkans (9) and in North African countries (10).…”

Section: Abstract: Bioactivation Carcinogenicity Dna Adduction Glmentioning

confidence: 99%

Glutathione Conjugates of Ochratoxin a as Biomarkers of Exposure / Glutationski Konjugati Okratoksina A Kao Biomarkeri Izloženosti

Tozlovanu

Canadas

Pfohl‐Leszkowicz

et al. 2012

Archives of Industrial Hygiene and Toxicology

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In the present study the photoreactivity of the fungal carcinogen ochratoxin A (OTA) has been utilised to generate authentic samples of reduced glutathione (GSH) and N-acetylcysteine (NAC) conjugates of the parent toxin. These conjugates, along with the nontoxic OTα, which is generated through hydrolysis of the amide bond of OTA by carboxypeptidase A, were utilised as biomarkers to study the metabolism of OTA in the liver and kidney of male and female Dark Agouti rats. Male rats are more susceptible than female rats to OTA carcinogenesis with the kidney being the target organ. Our studies show that the distribution of OTA in male and female rat kidney is not signifi cantly different. However, the extent of OTA metabolism was greater in male than female rats. Much higher levels of OTα were detected in the liver compared to the kidney, and formation of OTα is a detoxifi cation pathway for OTA. These fi ndings suggest that differences in metabolism between male and female rats could provide an explanation for the higher sensitivity of male rats to OTA toxicity.

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Section: Abstract: Bioactivation Carcinogenicity Dna Adduction Glmentioning

confidence: 99%

Glutathione Conjugates of Ochratoxin a as Biomarkers of Exposure / Glutationski Konjugati Okratoksina A Kao Biomarkeri Izloženosti

Tozlovanu

Canadas

Pfohl‐Leszkowicz

et al. 2012

Archives of Industrial Hygiene and Toxicology

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“…The genotoxicity of OTA has been postulated in vivo and in vitro (Creppy 2002). Genotoxic effects such as DNA strand breaks, sister chromatid exchanges, chromosomal aberrations and induction of micronuclei have been observed in some mammalian cell systems in 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 In North African countries, the most suspected food susceptible to be contaminated by OTA are domestic and imported cereals like wheat and sorghum, olives, poultry products and spices (Grosso et al, 2003). Data published suggested the evidence association of elevated exposure to OTA with cases of human nephropathies in Tunisia (Abid et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Incidence of ochratoxin A in rice and dried fruits from Rabat and Salé area, Morocco

Zinedine

Castillo

Juan

et al. 2007

Food Additives and Contaminants

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“…e Penicillium spp., cuja relevância se justifica pelo envolvimento em intoxicações animais e na etiologia de doenças renais em humano (FINK-GREMMELS;JAHN;BLOM, 1995;JODBAUER;MAIER;LINDNER, 2002). As propriedades toxicológicas de OTA incluem nefrotoxicidade, teratogenicidade, citotoxicidade e genotoxicidade (GROSSO et al, 2003). O limite máximo de OTA recomendado pela União Européia varia entre 5 a 20 ng/g para café verde, 3 a 10 ng/g para café torrado e 4 a 10 ng/g para café solúvel (FOOD AND AGRICULTURE ORGANIZATION OF UNITED NATIONS, 2006).…”

Section: Introductionunclassified

Espectrofluorimetria comparada à cromatografia líquida de alta eficiência na detecção de ocratoxina A em café

et al. 2008

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ResumoA cafeicultura brasileira apresenta grande importância ao mercado interno e comércio exterior. A qualidade e sanidade do café dependem da contaminação fúngica, com ênfase as espécies produtoras de ocratoxina A (OTA), uma nefrotoxina de efeito deletério à saúde pública. A necessidade de contínuo monitoramento e controle de OTA em produtos agrícolas requer metodologia analítica simples, rápida e acessível. O trabalho teve como objetivo padronizar um método espectrofluorimétrico para detecção de OTA visando monitoramento em grãos de café, correlacionando-o com a cromatografia líquida de alta eficiência (CLAE). A aplicabilidade da espectrofluorimetria foi avaliada em café artificialmente contaminado com OTA (10, 20 e 50 ng/g), comparando os resultados obtidos com a CLAE. Embora a fluorimetria tenha apresentado resultados insatisfatórios, com recuperação de 40,14 % e coeficiente de correlação de 0,79 em relação a CLAE, i.e. baixa confiabilidade e eficiência inadequada, a técnica constitui perspectiva para prosseguir o estudo inserindo novos ensaios referentes à sua padronização e certificação. Palavras-chave: Ocratoxina A, café, CLAE, espectrofluorimetria AbstractThe Brazilian coffee production plays important role both in the national and oversea trade. The quality and sanity of coffee depend on fungal contamination, with emphasis on ochratoxin A (OTA) producing species, which is a nephrotoxin with hazardous effect in public health. The exigency on continuous monitoring and control of OTA in agricultural commodity requires a simple, rapid and accessible analytical methodology. The objective of this research was to set up the spectrofluorimetric method for OTA detection intended for monitoring in coffee, which was developed in data-correlation with high efficiency liquid chromatography (HPLC). The performance of spectrofluorimetric analysis was evaluated using artificially contaminated coffee (10, 20 and 50 ng/g), and the data compared with HPLC. Although of the fault of former technique, with recovery of 40.14 % and correlation coefficient of 0.79 when compared to HPLC, i.e. low reliability and inadequate efficiency, the data showed perspective to follow the improvement inserting new tool, and further standardization and certification.

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New data on the occurrence of ochratoxin A in human sera from patients affected or not by renal diseases in Tunisia

Cited by 79 publications

References 26 publications

Glutathione Conjugates of Ochratoxin a as Biomarkers of Exposure / Glutationski Konjugati Okratoksina A Kao Biomarkeri Izloženosti

Glutathione Conjugates of Ochratoxin a as Biomarkers of Exposure / Glutationski Konjugati Okratoksina A Kao Biomarkeri Izloženosti

Incidence of ochratoxin A in rice and dried fruits from Rabat and Salé area, Morocco

Espectrofluorimetria comparada à cromatografia líquida de alta eficiência na detecção de ocratoxina A em café

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