New cytotoxic compounds from Myristica fatua Houtt leaves against MCF-7 cell lines

Fajriah, Sofa; Darmawan, Ahmad; Megawati, Megawati; Hudiyono, Sumi; Kosela, Soleh; Hanafi, Muhammad

doi:10.1016/j.phytol.2017.03.013

Cited by 13 publications

(7 citation statements)

References 14 publications

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“…Two neolignan compounds also well-recognized as resolcinols were isolated from the ethyl acetate fraction of the bark of M. fatua including malabaricone C and malabaricone B, which also possess known antiviral activities [19]. Other researchers reported novel cytotoxic lignan and diaryl nonanoid derivatives from the leaves of M. fatua [20,21]. Besides the action as antiviral agents, both compounds showed in vitro cytotoxic activity against the breast cancer cell lines MCF-7, with IC 50 of 26.19 and 8.33 mM, respectively [18].…”

Section: Discussionmentioning

confidence: 99%

The Antiviral Effect of Indonesian Medicinal Plant Extracts Against Dengue Virus In Vitro and In Silico

et al. 2019

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Dengue infections are still a worldwide burden, especially in Indonesia. There is no specific medication against the dengue virus. Recently, many types of research have been conducted to discover a new drug for dengue virus using natural resource extracts. Indonesia, as a tropical country, has a wide biodiversity. There are several medicinal plants in Indonesia that are believed to possess anti-dengue activity, such as Myristica fatua, Cymbopogon citratus, and Acorus calamus plants. We conducted an in vitro laboratory experiment of several extracts from Indonesian herbs combined with in silico analysis. The extracts were evaluated for safety and antiviral activity in Huh7it-1 cell lines, using a single dose of 20 µg/mL and dose-dependent (5, 10, 20, 40, 80 and 160 µg/mL) of plant extracts against dengue virus serotype 2 (DENV-2) NGC strain. The DMSO 0.1% was used as a negative control. The cytotoxic aspect was assessed by counting the cell viability, while the antiviral activity was calculated by counting the average inhibition. The selectivity index (SI) of plant extracts were performed from a ratio of CC50/EC50 value. In silico analysis was conducted to determine the free energy of binding between NS5 of dengue virus with bioactive compounds contained in Myristica fatua, Cymbopogon citratus and Acorus calamus extract plants. We determined that all extracts were not toxic against Huh7it-1 cell lines. The methanolic extracts of A. calamus, C. citratus, and M. fatua showed inhibition of DENV-2 at a dose of 20 µg/mL to 96.5%, 98.9%, and 122.7%, respectively. The dose-dependent effects showed that M. fatua has the best inhibition activity towards DENV-2. Molecular docking result showed that artesunic acid within M. fatua has the best free energy of binding (−7.2 kcal/mol), followed by homoegonol (−7.1 kcal/mol) which was slightly different from artesunic acid among others. The methanolic extracts of A. calamus, C. citratus, and M. fatua showed prospective anti-dengue activities both in vitro and in silico. Future research should be conducted to find the pure extracts of all useful herbs as a new candidate of antiviral drug.

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Section: Discussionmentioning

confidence: 99%

The Antiviral Effect of Indonesian Medicinal Plant Extracts Against Dengue Virus In Vitro and In Silico

et al. 2019

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“…The cytotoxic assay was conducted according to the method described (Fajriah et al, 2017;Megawati et al, 2016;Harneti et al, 2012). Firstly, MCF7 cells were incubated in the 96-well plates at an initial cell density of approximately 3 x10 4 cells cm -3 for 24 h. Various sample concentrations (6.25, 12.5, 25, 50, 100 and 200 µg mL -1 ) were then added.…”

Section: Cytotoxic Assaymentioning

confidence: 99%

Cytotoxic Activity of Flavonoid Compounds Isolated from Tetrameles Nudliflora Leaves

Megawati

Darmawan²,

Fajriah³

2020

Ann. Bogor.

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Tetrameles nudliflora is one of the endemic plants in Wallace region, especially in Mekongga Forest, Southeast Sulawesi, Indonesia. As part of our project in the field of natural drug discovery based on local bioresources, this study aimed to isolate secondary metabolite compounds from the ethyl acetate fractions of the leaves of T. nudiflora and evaluate their cytotoxic activities against breast carcinoma cancer lines, MCF-7. The methods used were various chromatography techniques such as vacuum column chromatography, gravitation column chromatography, thin layer chromatography, preparative thin layer chromatography, and re-crystallization using two different organic solvents. In the present study, two flavonoid compounds, kaempferol (1) and quercetin (2) were identified. The structures of 1-2 were determined using serial spectroscopic techniques, including mass spectrometry, one- and two-dimensional nuclear magnetic resonance. Moreover, using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) assay method, both compounds showed in vitro cytotoxic activity IC50 of 34.162 and 133.148 μg mL-1, respectively against MCF-7 cell lines.

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“…Not all Myristica species contain the diaryl nonanoids known as malabaricones, although they are in the same family. For example Myristica species such as M. alba , [14] M. andamania , [15] M. argentea , [7,16] M. maxima , [17] M. astrescens , [17,18] M. basilanica , [17,19] M. brachipoda , [17,18] M. brevistipes , [18,19] M. swamps , [20] M. fragrans , [21–32] M. maingayi , [33] M. dactyloides , [34–35] M. beddomei , [23] M. fatua , [36–38] M. magniflica , [19,39] M. phillipensis , [9,39] etc. do not contain malabaricones.…”

Section: Introductionmentioning

confidence: 99%

Anti‐Proliferative Acyl Phenols and Arylnonanoids from the Fruit Rind of Myristica malabarica Lam.

Bauri

Brodie

et al. 2022

Chemistry & Biodiversity

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Phytochemical investigation of the methanol extract of the fruit rind of Myristica malabarica led to the isolation of eight known compounds that were identified as malabaricones AÀ D, promalabaricones B and C, 1-(2,6-dihydroxyphenyl)tetradecan-1-one, and ericanone by comparison with literature spectroscopic data. The structures of malabaricones AÀ D, promalabaricone B, and 1-(2,6-dihydroxyphenyl)tetradecan-1-one were confirmed by X-ray crystallography. In vitro assay of the isolated phenols indicated that they exhibited moderate anti-proliferative activity against the A2780 human ovarian cancer cell. Compounds (1, 3, 5, 6 and 7) had the most potent activities, whereas the anti-proliferative activities of compounds 2 and 4 were less potent.

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New cytotoxic compounds from Myristica fatua Houtt leaves against MCF-7 cell lines

Cited by 13 publications

References 14 publications

The Antiviral Effect of Indonesian Medicinal Plant Extracts Against Dengue Virus In Vitro and In Silico

The Antiviral Effect of Indonesian Medicinal Plant Extracts Against Dengue Virus In Vitro and In Silico

Cytotoxic Activity of Flavonoid Compounds Isolated from Tetrameles Nudliflora Leaves

Anti‐Proliferative Acyl Phenols and Arylnonanoids from the Fruit Rind of Myristica malabarica Lam.

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