New conductive filament ready-to-use for 3D-printing electrochemical (bio)sensors: Towards the detection of SARS-CoV-2

Stefano, Jéssica S.; Silva, Luiz; Rocha, Raquel G.; Brazaca, Laís Canniatti; Richter, Eduardo M.; Muñoz, Rodrigo A.A.; Janegitz, Bruno C.

doi:10.1016/j.aca.2021.339372

Cited by 69 publications

(28 citation statements)

References 72 publications

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“…These methods require lengthy processes, skilled personnel, and centralized laboratories with expensive equipment. In this regard, miniaturized electrochemical systems, produced for example by screen printing and 3D printing methods, are promising for precision nutrition management as they can monitor health indicators in situ in a noninvasive or minimally invasive way. − In addition, they allow measurements with small sample volumes, being user-friendly to operate. Screen-printed electrodes can be manufactured on various substrates such as plastic, on the skin as a tattoo, and on surfaces such as gloves and mouthguards; moreover, they may be customized with their versatility in terms of size and geometry. − Electrochemical biosensors, including HRP-labeled biosensors, have been used for monitoring 25(OH)D3 (see Table ).…”

Section: Introductionmentioning

confidence: 99%

Label-Free Electrochemical Immunosensor Made with Tree-like Gold Dendrites for Monitoring 25-Hydroxyvitamin D3 Metabolite

Martins

Bott‐Neto

Machado

et al. 2022

ACS Appl. Mater. Interfaces

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Flexible, fully printed immunosensors can meet the requirements of precision nutrition, but this demands optimized molecular architectures to reach the necessary sensitivity. Herein, we report on flexible and label-free immunosensor chips made with tree-like gold dendrites (AuDdrites) electrochemically formed by selective desorption of l-cysteine (L-cys) on (111) gold planes. Electrodeposition was used because it is scalable and cost-effective for a rapid, direct growth of Au hyperbranched dendritic structures. The 25-hydroxyvitamin D3 (25(OH)D3) metabolite was detected within 15 min with a limit of detection (LOD) of 0.03 ng mL–1. This high performance was possible due to the careful optimization of the electroactive layer and working conditions for square wave voltammetry (SWV). Electrocrystallization was manipulated by controlling the deposition potential and the molar ratio between HAuCl4 and L-cys. Metabolite detection was performed on human serum and saliva samples with adequate recovery between 97% and 100%. The immunosensors were stable and reproducible, unresponsive to interference from other molecules in human serum and saliva. They can be extended for use as wearable sensors with their mechanical flexibility and possible customization.

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Section: Introductionmentioning

confidence: 99%

Label-Free Electrochemical Immunosensor Made with Tree-like Gold Dendrites for Monitoring 25-Hydroxyvitamin D3 Metabolite

Martins

Bott‐Neto

Machado

et al. 2022

ACS Appl. Mater. Interfaces

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“…The electrodes were then rinsed using MES buffer (100 mmol L −1 , pH 6.0) and dried using N 2 . Twenty microliters of 1 µg mL −1 anti-protein S antibodies (PBS, 0.1 mol L −1 , pH 7.0) was added to the electrode for 1 h. The antibody's concentration applied is within the concentration range reported in the literature for the manufacture of immunosensors used for SARS-CoV-2 detection, which range from 1 ng L −1 to 100 µg L −1 [31,32,[35][36][37][38][39][40]. Electrodes were washed using PBS (0.1 mol L −1 , pH 7.0) and dried with an N 2 gas stream.…”

Section: Functionalization Of the Gold Surface With Antibodiesmentioning

confidence: 99%

“…After washing the modified electrode with PBS (0.1 mol L −1 , pH 7.0) and drying it with N 2 , the biosensor was ready for use. A similar protocol for building the immunosensor was recently described by Stefano et al [40]. The samples were added to the working electrode and incubated for 30 min (0.1 mol L −1 PBS, pH 7.0).…”

Section: Functionalization Of the Gold Surface With Antibodiesmentioning

confidence: 99%

Electrochemical immunosensors using electrodeposited gold nanostructures for detecting the S proteins from SARS-CoV and SARS-CoV-2

et al. 2022

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This paper reports the development of a low-cost (< US$ 0.03 per device) immunosensor based on gold-modified screenprinted carbon electrodes (SPCEs). As a proof of concept, the immunosensor was tested for a fast and sensitive determination of S proteins from both SARS-CoV and SARS-CoV-2, by a single disposable device. Gold nanoparticles were electrochemically deposited via direct reduction of gold ions on the electrode using amperometry. Capture antibodies from spike (S) protein were covalently immobilized on carboxylic groups of self-assembled monolayers (SAM) of mercaptoacetic acid (MAA) attached to the gold nanoparticles. Label-free detection of S proteins from both SARS-CoV and SARS-CoV-2 was performed with electrochemical impedance spectroscopy (EIS). The immunosensor fabricated with 9 s gold deposition had a high performance in terms of selectivity, sensitivity, and low limit of detection (LOD) (3.16 pmol L −1 ), thus permitting the direct determination of the target proteins in spiked saliva samples. The complete analysis can be carried out within 35 min using a simple one-step assay protocol with small sample volumes (10 µL). With such features, the immunoplatform presented here can be deployed for mass testing in point-of-care settings.

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“…In this context, the search for new instruments and means of analysis that can circumvent these problems has intensified in recent decades. The search and development of new analytical devices have shown excellent results and high potential products, such as optical, fluorescent, colorimetric, and electrochemical devices [ 3 – 7 ]. In this aspect, the use of electrochemical techniques can be highlighted, being an alternative to conventional analytical methods, since they have several advantages which are currently required, mainly concerning cost, simplicity, miniaturization/portability capacity, and the possibility of on-site application [ 8 – 11 ].…”

Section: Introductionmentioning

confidence: 99%

Starch-Based Electrochemical Sensors and Biosensors: A Review

Vicentini

Silva

Stefano

et al. 2022

Biomedical Materials & Devices

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Natural green compounds for sensor modification (binders) are challenging in electrochemistry. Starch is a carbohydrate biopolymer that has been used extensively in the development of biomaterials for the food industry due to its ability to impart textural characteristics and provide gelling or film formation. In particular, the excellent film-forming characteristics have been used for the development of new surface modifying architectures for electrodes. Here, we highlight a very comprehensive overview of the properties of interest of various types of starch in conjunction with (bio)materials in the chemical modification of sensors and biosensors. Throughout the review, we first give an introduction to the extraction, applications, and properties of starches followed by an overview of the prospects and their possible applications in electrochemical sensors and biosensors. In this context, we discuss some important characteristics of starches and different strategies of their film formation with an emphasis on their role in the development of electrochemical sensors and biosensors highlighting their main contributions to enhancing the performance of these devices and their applications in environmental and clinical samples. Graphical Abstract

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New conductive filament ready-to-use for 3D-printing electrochemical (bio)sensors: Towards the detection of SARS-CoV-2

Cited by 69 publications

References 72 publications

Label-Free Electrochemical Immunosensor Made with Tree-like Gold Dendrites for Monitoring 25-Hydroxyvitamin D3 Metabolite

Label-Free Electrochemical Immunosensor Made with Tree-like Gold Dendrites for Monitoring 25-Hydroxyvitamin D3 Metabolite

Electrochemical immunosensors using electrodeposited gold nanostructures for detecting the S proteins from SARS-CoV and SARS-CoV-2

Starch-Based Electrochemical Sensors and Biosensors: A Review

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