New Colorimetric Reaction for End-Point, Continuous-Flow, and Kinetic Measurement of Urea

Jung, D.H.; Biggs, Homer G.; Erikson, John M.; Ledyard, Peggy U.

doi:10.1093/clinchem/21.8.1136

Cited by 136 publications

(89 citation statements)

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“…This reaction was competitive by antagonized by amino residue of protein in reaction mixture. If protein concentration may decrease in reaction mixture due to dilution by physiological saline solution, reaction of urea with o-Phthalaldehyde will increase (2). Therefore, when samples need to be diluted, 7 % bovine serum albumin should be used for dilutions.…”

Section: Discussionmentioning

confidence: 99%

Application to Cows and Horses of Spotchem, a Dry‐Chemistry Blood Analyzer for Use in Veterinary Clinics

Hoshi

Satho

Koyama

et al. 1994

Journal of Veterinary Medicine Series A

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Summary The usefulness of a dry‐chemistry blood analyzer, Spotchem SP‐4410 (SP‐4410) in a veterinary clinic for analysis of bovine and equine blood chemistry was studied. We quantitated total protein (TP), albumin (Alb), total bilirubin (T‐Bil), blood urea nitrogen (BUN), total cholesterol (T‐Cho), glucose (Glu), calcium (Ca), aspartate aminotransferase (AST), gamma‐glutamyltransferase (GGT), creatinine phosphokinase (CPK), and alkaline phosphatase (ALP) in bovine sera. Each sample was assayed with both the SP‐4410 and an automated blood analyzer which served as a wet‐chemistry reference system, and the data were analyzed with regression analysis. The correlation coefficient for AST was 0.997 being the highest for all the parameters, and all the correlation coefficients were 0.93 or higher. The coefficients of variation were lower than 5.0 except in the case of bovine T‐Bil where it was 5.756. The ranges of normal reference values measured by SP‐4410 were the same as those reported by other investigators in most cases, but those for GGT and CPK were slightly higher. The strongest interference was observed with hemoglobin. It seems that dry‐chemical‐analysis of blood serum using the SP‐4410 is useful for analysis of bovine and equine blood.

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Section: Discussionmentioning

confidence: 99%

Application to Cows and Horses of Spotchem, a Dry‐Chemistry Blood Analyzer for Use in Veterinary Clinics

Hoshi

Satho

Koyama

et al. 1994

Journal of Veterinary Medicine Series A

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“…Using commercially available kits (Sigma-Aldrich, St. Louis, MO), the activity of aspartate aminotransferase (AST)/glutamate oxalate transaminase (GOT), alanine aminotransferase (ALT)/glutamate pyruvate transaminase (GPT), bilirubin, creatinine, and urea levels was determined. [35][36][37][38][39]…”

Section: In Vivo Antioxidative Hypocholesterolemic and Organ Functimentioning

confidence: 99%

Evaluation of the Antioxidative and Hypo-cholesterolemic Effects of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), in Ameliorating Cardiovascular Disease

Rahman

Abdullah

Aminudin

2018

Int J Med Mushrooms

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Oxidative stress (OS) and hypercholesterolemia have been linked with a heightened risk of cardiovascular disease (CVD). Because of the numerous drawbacks of synthetic antioxidants and cholesterol-lowering drugs, natural antioxidative and hypocholesterolemic agents are of immense importance. This study was designed to determine both the OS-attenuating and cholesterol-lowering capacities of the hot water extract (HWE) and of five solvent-solventpartitioned fractions of Ganoderma lucidum. In vitro antioxidative performance of G. lucidum HWE and fractions was measured through DPPH free radical scavenging, Folin-Ciocalteu assay, lipid peroxidation inhibition, and human low-density lipoprotein (LDL) oxidation inhibition. In vivo antioxidative performance of G. lucidum was assessed by measuring the plasma and liver antioxidative enzymatic activities (catalase, glutathione peroxidase, and superoxide dismutase) in G. lucidum HWE-fed rats. In the CVD tests, the HWE at 200 mg/kg b.w. lowered plasma levels of total cholesterol, triacylglycerol, and LDL cholesterol and increased high-density lipoprotein cholesterol. The current findings indicate the therapeutic potentiality of G. lucidum as an OS-attenuating and hypocholesterolemic agent en route to withstanding CVD complications.

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“…Hematological parameters {packed cell volume (PCV), hemoglobin concentration (HBC), total white blood cell count (TWBC)} were analyzed as described by Dacie and Lewis [9], plasma urea was determined by the method described by Jung et al [10] and creatinine estimation was done by method originally described by Benedict and Behie [11] and reevaluated by Stevens et al [12], plasma potassium (K + ) and sodium (Na + ) were determined by flame atomic absorption spectrophotometer, bicarbonate (HCO3 -) was determined by titration in accordance with the method described by Van Slyke [13], Serum chloride (Cl -) was estimated by the mercuric nitrate colorimetric method described by Skeggs and Hochstrasser [14].…”

Section: Sample Analysesmentioning

confidence: 99%

Hemoprotective and nephroprotective potentials of aqueous extract of Jussiaea nervosa leaf in cadmium exposed albino rats.

Ibiam¹

2012

IOSJPBS

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In this study, the hemoprotective and nephroprotective potentials of aqueous extract of Jussiaea nervosa leaves on cadmium-exposed rats were evaluated. In the first experiment, 24 Wister albino rats divided into 6 groups: A-F of 4 rats per group were used for the evaluation of Cd toxicity. While group A rats received water and feed only and served as negative control (NC), groups B-F received in addition to water and feed, 1, 2, 4, 8 & 12 mg/kg body weight of Cd, respectively for 6 weeks. Based on the significant effect of Cd at 12mg/kg body weight in the first experiment, additional group (G), exposed to 12mg/kg body weight of Cd was established for the assessment of the hemoprotective and nephroprotective potentials of J. nervosa in Cdexposed rats. This group (n = 12) was subdivided into four subgroups (G1, G2, G3 and G4) of 3 rats each. Subgroup G1 was maintained on normal feed and water and served as the positive control (PC) while G2, G3 and G4 were given 20, 50 and 100 mg/kg doses of aqueous Jussiaea nervosa extract, respectively. All feedings and treatment also lasted for six weeks. At the end of the experiment, the animals were sacrificed and blood and kidney samples were collected for laboratory analyses. Biochemical and histological analyses were done using standard laboratory techniques. Exposure to cadmium was observed to cause significant (p < 0.05) decreases in packed cell volume (PCV), hemoglobin concentration (HBC), and total white blood cell count (TWBC) and increases in plasma urea, creatinine & electrolytes. However, administration of aqueous extract of J. nervosa ameliorated these Cd-induced effects in a dose-related manner, with higher doses (50 mg/l & 100 mg/l) almost restoring the parameters to the levels in non-exposed rats. Additionally, the Cd-induced changes in the histology of the kidneys were almost restored by administration of Jussiaea nervosa extract. We conclude that J. nervosa may possess haemoprotective and nephroprotective potentials and its regular consumption may protect against cadmium toxicity.

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New Colorimetric Reaction for End-Point, Continuous-Flow, and Kinetic Measurement of Urea

Cited by 136 publications

References 0 publications

Application to Cows and Horses of Spotchem, a Dry‐Chemistry Blood Analyzer for Use in Veterinary Clinics

Application to Cows and Horses of Spotchem, a Dry‐Chemistry Blood Analyzer for Use in Veterinary Clinics

Evaluation of the Antioxidative and Hypo-cholesterolemic Effects of Lingzhi or Reishi Medicinal Mushroom, Ganoderma lucidum (Agaricomycetes), in Ameliorating Cardiovascular Disease

Hemoprotective and nephroprotective potentials of aqueous extract of Jussiaea nervosa leaf in cadmium exposed albino rats.

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