New Capability of Electroinjection Analysis:  Investigation of Chemical Reaction Kinetics

Abstract

Electroinjection analysis has been demonstrated to provide reaction kinetics information. Reactants are injected continuously from opposite ends of the capillary for the duration of the experiment, and reaction product formation occurs at the boundary where the zones merge. The width of the product signal measured by the detector depends on the ratio of the fluxes of reactants. The boundary moves toward the detector where the products are measured. A sharp peak at the edge of the main flat product signal peak was observed and most probably corresponds to the metastable intermediate product of reaction, a unique capability of this technique. A theoretical explanation and an experimental verification of the possibile use of electroinjection analysis for the study of reaction kinetics is given.

Nanopore Event-Transduction Signal Stabilization for Wide pH Range under Extreme Chaotrope Conditions

“…CE may be employed for analysis of fast chemical reactions (fast decay, etc. ) [34,35,36]. Similar to chromatographic separation, capillary electrophoresis provides an opportunity to determine reaction kinetics [31,34,35,36] although the accuracy of these calculations is not very high.…”

“…[34,35,36]. Similar to chromatographic separation, capillary electrophoresis provides an opportunity to determine reaction kinetics [31,34,35,36] although the accuracy of these calculations is not very high. The CE technique also has certain advantages due to its suitability for study of complex formation at different pH and in presence of additives modulating the interaction (salt ions and other charged compounds).…”

Advances in capillary electrophoretically mediated microanalysis

“…Andreev et al [44][45][46][47] presented another variant of EMMA, which they referred to as electroinjection analysis (EIA). In this approach, the two reactants of interest are injected simultaneously from opposite ends of the capillary using electrokinetic injection.…”