New Butyroside D from Argan Press Cake Possess Anti-Melanogenesis Effect via MITF Downregulation in B16F10 and HEM Cells

Bouhoute, Meryem; Amen, Yhiya; Bejaoui, Meriem; Oliva, Aprill Kee; Shimizu, Kuniyoshi; Isoda, Hiroko

doi:10.3390/ijms232416021

Cited by 2 publications

(2 citation statements)

References 67 publications

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“…Melanin synthesis in pigmented cells is regulated by a complex and interconnected set of intracellular signaling pathways, including PKA (stimulated by cAMP elevators), PKC (stimulated by endothelin) and MAPK (ERKs; stimulated by growth factors). The cAMP plays a pivotal role in the regulation of melanogenesis through the activation of PKA and cAMP responsive element binding protein (CREB), which led to the upregulation of the expression of microphthalmia-associated transcription factor (MITF) [48].…”

Section: Camp Is Reduced By Melatonin and Its Metabolites Independent...mentioning

confidence: 99%

Melanogenesis Is Directly Affected by Metabolites of Melatonin in Human Melanoma Cells

Möller,

Linowiecka,

Gagat

et al. 2023

IJMS

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Melatonin (N-acetyl-5-methoxytryptamine, MEL), its kynurenic (N1-acetyl-N2-formyl-5-methoxykynurenine, AFMK) and indolic derivatives (6-hydroxymelatonin, 6(OH)MEL and 5-methoxytryptamine, 5-MT) are endogenously produced in human epidermis. Melatonin, produced by the pineal gland, brain and peripheral organs, displays a diversity of physiological functions including anti-inflammatory, immunomodulatory, and anti-tumor capacities. Herein, we assessed their regulatory effect on melanogenesis using amelanotic (A375, Sk-Mel-28) and highly pigmented (MNT-1, melanotic) human melanoma cell lines. We discovered that subjected compounds decrease the downstream pathway of melanin synthesis by causing a significant drop of cyclic adenosine monophosphate (cAMP) level, the microphthalmia-associated transcription factor (MITF) and resultant collapse of tyrosinase (TYR) activity, and melanin content comparatively to N-phenylthiourea (PTU, a positive control). We observed a reduction in pigment in melanosomes visualized by the transmission electron microscopy. Finally, we assessed the role of G-protein-coupled seven-transmembrane-domain receptors. Obtained results revealed that nonselective MT1 and MT2 receptor antagonist (luzindole) or selective MT2 receptor antagonist (4-P-PDOT) did not affect dysregulation of the melanin pathway indicating a receptor-independent mechanism. Our findings, together with the current state of the art, provide a convenient experimental model to study the complex relationship between metabolites of melatonin and the control of pigmentation serving as a future and rationale strategy for targeted therapies of melanoma-affected patients.

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Section: Camp Is Reduced By Melatonin and Its Metabolites Independent...mentioning

confidence: 99%

Melanogenesis Is Directly Affected by Metabolites of Melatonin in Human Melanoma Cells

Möller,

Linowiecka,

Gagat

et al. 2023

IJMS

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“…Even in different organs and tissues of the same species, TYR has different functions [ 10 ]. The influence of samples on the activity of mushroom TYR (MTYR) [ 5 , 11 ] and TYR extracted from animal cells [ 12 , 13 ] are commonly used indicators for the preliminary screening of samples with potential anti-melanogenic activity. Based on the above species differences among TYR, we are considering whether a sample have consistent effects on enzyme activities of different species, and whether this result could be further promoted for production application or clinical research.…”

Section: Introductionmentioning

confidence: 99%

Inhibitory Activities of Samples on Tyrosinases Were Affected by Enzyme Species and Sample Addition Methods

Wang

Yang

Wang

et al. 2023

IJMS

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The inhibition of tyrosinase (TYR) activity is an effective measure to inhibit melanin synthesis. At present, there are many methods with discrepant details that study the TYR inhibitory activity of samples. Under the same experimental conditions, this paper systematically studies whether enzyme species and sample addition methods are the key factors that determine the TYR inhibitory activity of samples. TYRs extracted from B16F10 cells, apple and mushroom, called BTYR, ATYR and MTYR, respectively, were selected to implement this study. Results showed that TYR inhibitory activities of samples were obviously affected by the above two factors. It was necessary to select the appropriate enzyme according to the problems to be explained. It was speculated that indirectly inhibitory activity reflected the comprehensive effects of samples on TYR catalytic activity and intracellular TYR synthesis pathway, while directly inhibitory activity reflected the effects of samples on TYR catalytic activity. Additionally, kojic acid could be used as a positive control for both B16F10 cells and MTYR models. The TYR inhibitory activity of β-arbutin was complicated and fickle, while that of epigallocatechin gallate (EGCG) was universal and stable, which is to say, EGCG always inhibited TYR activity in a dose-dependent manner. In conclusion, the TYR inhibitory activities of samples were affected by enzyme species and sample addition methods. Compared with the unstable β-arbutin, EGCG was more valuable for clinical research.

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New Butyroside D from Argan Press Cake Possess Anti-Melanogenesis Effect via MITF Downregulation in B16F10 and HEM Cells

Cited by 2 publications

References 67 publications

Melanogenesis Is Directly Affected by Metabolites of Melatonin in Human Melanoma Cells

Melanogenesis Is Directly Affected by Metabolites of Melatonin in Human Melanoma Cells

Inhibitory Activities of Samples on Tyrosinases Were Affected by Enzyme Species and Sample Addition Methods

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