New biorecognition molecules in biosensors for the detection of toxins

Bazin, Ingrid; Tria, Scherrine A.; Hayat, Akhtar; Marty, Jean‐Louis

doi:10.1016/j.bios.2016.06.083

Cited by 161 publications

(82 citation statements)

References 208 publications

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“…One approach to mitigate the health risks posed by OTA is to impose strict regulatory limits on the OTA levels in food matrices to control its bioaccumulation in the consumer. Currently, antibodies provide the specificity and sensitivity required for monitoring OTA levels (limit of detection <2.5 nM) in food, animal feed, and beverages [5,6,7]. Antibodies are widely used in immunoaffinity columns, enzyme-linked immunosorbent assays (ELISAs), test strips, and various biosensor formats [5,6,7].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Ochratoxin Recognition by Peptides Using Explicit Solvent Molecular Dynamics

Thyparambil

Bazin²,

Guiseppi‐Elie

2017

Toxins

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Biosensing platforms based on peptide recognition provide a cost-effective and stable alternative to antibody-based capture and discrimination of ochratoxin-A (OTA) vs. ochratoxin-B (OTB) in monitoring bioassays. Attempts to engineer peptides with improved recognition efficacy require thorough structural and thermodynamic characterization of the binding-competent conformations. Classical molecular dynamics (MD) approaches alone do not provide a thorough assessment of a peptide’s recognition efficacy. In this study, in-solution binding properties of four different peptides, a hexamer (SNLHPK), an octamer (CSIVEDGK), NFO4 (VYMNRKYYKCCK), and a 13-mer (GPAGIDGPAGIRC), which were previously generated for OTA-specific recognition, were evaluated using an advanced MD simulation approach involving accelerated configurational search and predictive modeling. Peptide configurations relevant to ochratoxin binding were initially generated using biased exchange metadynamics and the dynamic properties associated with the in-solution peptide–ochratoxin binding were derived from Markov State Models. Among the various peptides, NFO4 shows superior in-solution OTA sensing and also shows superior selectivity for OTA vs. OTB due to the lower penalty associated with solvating its bound complex. Advanced MD approaches provide structural and energetic insights critical to the hapten-specific recognition to aid the engineering of peptides with better sensing efficacies.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Ochratoxin Recognition by Peptides Using Explicit Solvent Molecular Dynamics

Thyparambil

Bazin²,

Guiseppi‐Elie

2017

Toxins

Self Cite

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“…Moreover, the evolution of synthetic biology that enables us to modify the genetic code of biological systems and to reprogram them to perform new tasks will help us to overcome challenges such as lowering the detection limits, increasing sensitivity, and lowering production cost. In addition, synthetic materials, genetically-engineered molecules, aptamers and artificial membranes have recently received great attention as novel biosensing materials with improved performance and/or additional functions [15,16]. Poor stability of biological molecules could be overcome by developing artificial molecular recognition elements with the desired selectivity and sensitivity towards various compounds.…”

Section: Discussionmentioning

confidence: 99%

Biosensor-Based Approaches for Detecting Ochratoxin A and 2,4,6-Trichloroanisole in Beverages

Mavrikou

Kintzios

2018

Beverages

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Mycotoxins and haloanisoles are secondary metabolites produced under special conditions of temperature and humidity by fungi colonizing a variety of commodities from preharvest up to consumer use. Ochratoxin A and 2,4,6-trichloanisole are produced mainly by species of the genus Aspergillus and Penicillium. Ochratoxin A exhibits nephrotic effects and can, potentially, be associated with human carcinogenesis, whereas 2,4,6-trichloanisole is primarily responsible for cork taint in wines. This review provides an overview of recent advances in biosensor technology for the determination of the aforementioned compounds in wine, beer and other beverages, as well as cork stoppers, which help in establishing and carrying out proper product quality-management strategies. Such a detailed investigation of biosensor-based detection methods of these toxic compounds in beverages could lead to the provision of safe-to-consume products, and allow the prioritization of future research efforts.

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“…In the recent years, nanoparticle-based techniques for DNA detection has developed [67]. The small labelled size, bio-conjugation ability and the unusual optical and electrical properties of metal nanoparticles make them unique tools for DNA detection [68]. Semiconductor nanoparticles have been extensively used as labels in electrochemical biosensors, especially DNA sensors.…”

Section: Iron Oxide-based Nanomaterialsmentioning

confidence: 99%

Nanozyme applications in biology and medicine: an overview

Golchin

Alidadian

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

103

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Nanozymes, in nature, are artificial enzymes. Innovated by Ronald Breslow to mimic enzymes. Nanozymes have widespread applications including targeted cancer therapy, diagnostic medicine and bio-sensing even environmental toxicology. However, these applications are a novel research field in biomedicine, but are growing fast. Enzyme-based applications such as immune-absorbent assay (ELIZA) are expensive because of the complexity of producing enzymes and antibodies. Not only, some nanoparticles can mimic these enzymes such as superoxides, but also they can manipulate biological pathways directly like autophagy. These abilities make them a suitable alternative for both therapy and diagnosis. In this review, we opted on metal nanoparticles and application of this cutting edged technology into modern medicine.

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New biorecognition molecules in biosensors for the detection of toxins

Cited by 161 publications

References 208 publications

Evaluation of Ochratoxin Recognition by Peptides Using Explicit Solvent Molecular Dynamics

Evaluation of Ochratoxin Recognition by Peptides Using Explicit Solvent Molecular Dynamics

Biosensor-Based Approaches for Detecting Ochratoxin A and 2,4,6-Trichloroanisole in Beverages

Nanozyme applications in biology and medicine: an overview

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