New approaches to generate disease-resistant Gladiolus

Nasir, Idrees Ahmad; Riazuddin, Sheikh

doi:10.1007/s11274-007-9484-6

Cited by 5 publications

(3 citation statements)

References 15 publications

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“…Problems in plant regeneration combined with in vitro selection for a longer period might be associated with cytogenetic alteration (McCoy et al, 1982). Similarly, exposure of cell lines of Gladiolus to fusaric acid for a longer period resulted in cytogenetic alteration (Nasir and Riazuddin, 2008). In most instances, the long-term application of fungal culture filtrates may induce a long-lasting and extensive spectrum of natural disease resistance in plants grown under non-stressed field conditions, as described by Vidhyasekaran (1997).…”

Section: Effect Of Fungal Culture Filtrate On Plant Regeneration Via ...mentioning

confidence: 97%

Evaluation of rust disease tolerance and antioxidant enzyme activity from organogenesis and somatic embryogenesis in safflower (Carthamus tinctorius L.) cv. NARI-H-15

2022

ANRES

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Importance of the work: Rust fungi (Puccinia carthami) play a critical role as major threats to diminishing safflower growth, quality, and productivity worldwide. Therefore, there is a need to develop and deploy rust-resistant safflower plants. Recently, in vitro selection of fungal toxins has been an effective tool for disease resistance in many crops. Objectives: The present study aimed to standardize an efficient protocol for rust-resistant safflower (Carthamus tinctorius L.) cv. NARI-H-15 plant development through organogenesis and embryogenesis processes using P. carthami fungal culture filtrates (FCFs) as selection pressure. Materials & Methods:In vitro FCF selection technique with organogenesis and somatic embryogenesis pathways were used for the experimental purpose to develop tolerant plants. Antioxidant enzyme and pathogenicity assay methods were applied to determine the level of tolerance against biotic stress. Results: For organogenesis, immature leaf calli cultured on 40% FCF-selected Murashige and Skoog (MS) medium induced 22.8% more shoot organogenesis and 16.6% more rooting than cotyledonary leaf calli. For somatic embryogenesis, cotyledon-derived embryogenic calli cultured on 40% FCF-selected MS medium produced 30% more somatic embryogenesis with 17% more germination than immature-leaf-derived embryogenic calli. Fully regenerated FCF-tolerant plants were transferred to pots containing a soil mixture, with 52% of plants survived under greenhouse conditions. Further experiments evaluated the reactive oxygen species scavenging enzymes in FCF-tolerant plants. Catalase activity decreased while peroxidase and superoxide dismutase activities were higher in the FCF-tolerant plants than for the control, based on quantitative and qualitative analysis. The pathogenicity results confirmed the disease resistance of the in vitrodeveloped FCF-treated plants. Main finding: Application of 40% FCF in tissue culture medium appears to be the reliable strategy for enhancing rust disease resistance in safflower cv. NARI-H-15 against P. carthami.

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Section: Effect Of Fungal Culture Filtrate On Plant Regeneration Via ...mentioning

confidence: 97%

Evaluation of rust disease tolerance and antioxidant enzyme activity from organogenesis and somatic embryogenesis in safflower (Carthamus tinctorius L.) cv. NARI-H-15

2022

ANRES

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“…The disease can be controlled by spraying the plants with fungicide. The continuous use of fungicides proved to be hazardous, polluting the environment and leading to residual toxicity [5], creating resistance in pathogens and reducing soil fertility [6]. The manipulation of inherent potential of plants in the form of resistant varieties is a cheap, viable, environment friendly and alternative to reduce losses from biotic stress.…”

Section: Introductionmentioning

confidence: 99%

Screening of China Aster [Callistephus chinensis (L.)] Genotypes and F1 Hybrids against Alternaria Leaf Spot Disease

Chanu

Shiragur²,

Nishani³

et al. 2022

IJPSS

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Leaf spot disease caused by Alternaria alternata (Fr.) Keissler is a threat of China aster cultivation and is capable of causing yield losses in all production regions. The experiment was undertaken to screen genotypes and F1 hybrids which would be helpful in developing Alternaria leaf spot resistance varieties in later years. AAC-1 was found to be resistant against Alternaria leaf spot in both field and control conditions. In natural disease pressure, Arka Kamini, Arka Shashank and Arka Poornima recorded to be moderately susceptible, susceptible and highly susceptible to Alternaria leaf spot, respectively. However, in artificially inoculated condition, Arka Kamini showed susceptible reaction. Among F1 hybrids, AAC-1 x Arka Kamini and Arka Kamini x AAC-1 showed moderately resistant reaction; AAC-1 x Arka Poornima, AAC-1 x Arka Shashank, Arka Poornima x AAC-1, Arka Shashank x AAC-1 showed moderately susceptible disease reaction for Alternaria leaf spot.

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“…Micro-propagated plants are raised under sterile conditions that avoid pathogens and induce rapid multiplication. They further maintain clonal and phenotype uniformity among the offspring, round the year production and the disease free plants make the technology much more acceptable (5). Tissue culture techniques are used as routine procedures to obtain large amounts of good quality planting material (6).…”

Section: Introductionmentioning

confidence: 99%

Development and Optimization of MicroPropagation, In Vitro Methodology for Gladiolus

Mateen

2019

BSR

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Gladiolus is a non-native, costly but an important flowering plant in South Asia. Considering its lower production rate and economic importance, micro-propagation technology was optimized in this study to establish a protocol for pathogen free clonal genotypes. Taken the results together, the best response was observed at 27°C as growing temperature, table sugar (sucrose) as a carbon source, apical Keywords BAP IBA Kinetin Micro-propagation MS basal medium NAA meristem of 3mm for shoot formation as an explant. The best plant regeneration was observed for shoot apical meristem (within 7.8 days after inoculation) by using Murashige and Skoog MS basal medium with supplementing 1.0 mg/L BAP and 0.5 mg/L of Kinetin. The best shoot multiplication was 98% in 18.2 days of inoculation using MS media with 1.0 mg/L BAP only. For root induction, MS media which was supplemented with 1.0 mg/L NAA and 2.0 mg/L IBA gave the best results (100% in 6.8 days). The best supporting agent was found to be cotton swab while the best media for hardening and acclimatization of well-developed plants was a combination of sand, soil, and peat in an equal ratio (1:1:1), leading to 80% survival rate of plants. The results obtained in this study may better help grow Gladiolus plants on an industrial scale with a lower cost of production Bioscientific Reviews Volume 1(2) Bioscientific Reviews Volume 1(2)

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New approaches to generate disease-resistant Gladiolus

Abstract: Fusarium oxysporum is an important pathogen that can reduce corm and flower production of Gladiolus.

Cited by 5 publications

References 15 publications

Evaluation of rust disease tolerance and antioxidant enzyme activity from organogenesis and somatic embryogenesis in safflower (Carthamus tinctorius L.) cv. NARI-H-15

Evaluation of rust disease tolerance and antioxidant enzyme activity from organogenesis and somatic embryogenesis in safflower (Carthamus tinctorius L.) cv. NARI-H-15

Screening of China Aster [Callistephus chinensis (L.)] Genotypes and F1 Hybrids against Alternaria Leaf Spot Disease

Development and Optimization of MicroPropagation, In Vitro Methodology for Gladiolus

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