New Approaches to Cassava Breeding

Ceballos, Hernán; Hershey, Clair H.; Becerra‐López‐Lavalle, Luis Augusto

doi:10.1002/9781118358566.ch6

Cited by 52 publications

(67 citation statements)

References 172 publications

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“…In the former, the identity of both (male and female) progenitors is known. In the latter, only the female progenitor is known [78]. Large number of botanical seed from the crosses of elite germplasm is generated each year.…”

Section: Evolution and Improvements Of Breeding Methods To Increase Cmentioning

confidence: 99%

“…Separation and quantification of carotenoids are achieved at CIAT using an YMC Carotenoid S-5 C30 reversed-phase column (4.6 mm × 150 mm: particle size, 5 μm), with a YMC carotenoid S-5 guard column (4.0 × 23 mm) in a HPLC, using DAD detector with wavelength set at 450 nm. Peaks are identified by comparing retention time and spectral characteristics against a pure standard and available literature [78].…”

Section: Evolution and Improvements Of Quantifying Protocols For Caromentioning

confidence: 99%

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Carotenoids in Cassava Roots

Ceballos¹,

Davrieux²,

Talsma³

et al. 2017

Carotenoids

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Vitamin A deficiency (VAD) is a preventable tragedy that affects millions of people, particularly in sub-Saharan Africa. A large proportion of these people rely on diets based on cassava as a source of calories. During the last two decades, significant efforts have been made to identify sources of germplasm with high pro-vitamin A carotenoids (pVAC) and then use them to develop cultivars with a nutritional goal of 15 μg g −1 of β-carotene (fresh weight basis) and good agronomic performance. The protocols for sampling roots and quantifying carotenoids have been improved. Recently, NIR predictions began to be used. Retention of carotenoids after different root processing methods has been measured. Bioavailability studies suggest high conversion rates. Genetic modification has also been achieved with mixed results. Carotenogenesis genes have been characterized and their activity in roots measured.

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Section: Evolution and Improvements Of Breeding Methods To Increase Cmentioning

confidence: 99%

Section: Evolution and Improvements Of Quantifying Protocols For Caromentioning

confidence: 99%

Carotenoids in Cassava Roots

Ceballos¹,

Davrieux²,

Talsma³

et al. 2017

Carotenoids

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“…Only 464 genotypes out of the 473 were eventually harvested and analyzed. Since there is considerable within-family variation in full sib families of cassava (Ceballos et al 2012), each plant was evaluated independently. The progenitors were also evaluated.…”

Section: Field Evaluation and Data Collectionmentioning

confidence: 99%

“…In cassava breeding, parental lines are usually selected based on their performance per se and little progress has been made using general combining ability as a criterion for parental selection (Ceballos et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Parent-offspring regression analysis for total carotenoids and some agronomic traits in cassava

et al. 2015

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Six cassava cultivars, three yellow and three white-fleshed roots were crossed in a 3 9 3 topcross mating design to generate nine F 1 populations. One thousand, one hundred and ten botanical seeds from the 9 populations were sown in pots and maintained for 42 days in a screenhouse. The emerged seedlings were transplanted to the field in April, 2010 alongside their parents (from stem cuttings), family by family. Four hundred and sixty-four progenies survived and were harvested. Both field and laboratory data were used to evaluate total carotene content (TCC), dry matter content (DMC), storage fresh root yield (SFRY) and other root quality traits. Seed germination for different populations ranged between 15.5 and 80.9 % with a mean of 43.19 %. Phenotypic variation in DMC, TCC, SFRY, biomass, root number, harvest index and cassava mosaic disease (CMD) were recorded in all the families. Average values for the populations were TCC: 4.59 lg g -1 , DMC, 33.58 % and SFRY, 18.42 t ha -1 . Narrow sense heritability by midparent-offspring regression analysis and genetic gains were estimated for TCC, DMC, SFRY and reaction to CMD. TCC, DMC and CMD gave high heritability estimates of 0.73, 0.83 and 0.84, respectively. SFRY, on the other hand, had a low heritability estimate (0.15). TCC was negatively correlated with DMC across all evaluation stages and locations. There were very high levels of variation in the segregating F 1 progenies for all the traits. Also, narrow sense heritability estimate showed that genetic factors played a more important role than environmental factors for TCC, DMC and CMD, suggesting that reliable selection with simple recurrent phenotypic selection would be rewarding.

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“…Such trait-associated markers, after validation in different genetic backgrounds, can be implemented in GAB. Although several success stories of GAB are available in many crops, mainly cereals (Sundaram et al, 2008;Ceballos et al, 2012;Miedaner and Korzun, 2012;Singh et al, 2012), some examples have become available in some legume crops, e.g., soybean, peanut, chickpea, etc. (Tar'an et al, 2003;Miklas et al, 2006;Muehlbauer et al, 2006;Timko et al, 2011).…”

mentioning

confidence: 99%