New approach to quantitative analysis of benzo[a]pyrene in food supplements by an immunochemical column test

Beloglazova, Natalia V.; Goryacheva, Irina Yu.; Saeger, Sarah De; Scippo, Marie Louise; Knopp, Dietmar

doi:10.1016/j.talanta.2011.03.052

Cited by 12 publications

(6 citation statements)

References 23 publications

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“…The frit-based column immunoassay procedure was described in our previous publications [32,33]. First, polyethylene frits were degassed in ethanol by ultrasonication (15 min).…”

Section: Polyethylene Frit-based Immunoassay (Fbi) For On-site Detectionmentioning

confidence: 99%

Liposomes loaded with quantum dots for ultrasensitive on-site determination of aflatoxin M1 in milk products

et al. 2013

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A quantitative fluorescence labeled immunosorbent assay (FLISA) and qualitative onsite column tests were developed for the determination of aflatoxin M1 (AfM1) in milk products. The use of quantum dots (QD) loaded liposomes as a label significantly increased the assay sensitivity by encapsulating multiple QD in a single liposome and, therefore, amplifying the analytical signal.Two different techniques were compared to obtain aflatoxin-protein conjugates, used for further coupling with the liposomes. The influence of non-specific interactions of the obtained liposomes-labeled conjugates with the surface of microtiter plates and column cartridges was evaluated and discussed. The limit of detection for FLISA was 0.014 µg kg -1 .For qualitative on-site tests the cut-off was set at 0.05 µg kg -1 taking into account the EU maximum level for aflatoxin M1 in raw milk, heat-treated milk and milk for the manufacture of milk based product. The direct addition of labeled conjugate in the milk samples resulted in an additional decrease of analysis time.An intra-laboratory validation was performed with sterilized milk and cream samples artificially spiked with AfM1 at concentrations less, equal and more than the cut-off level. It was shown that milk products could be analyzed without any sample preparation, just diluted with the buffer. The rates for false positive and false negative results were below 5% (2.6% and 3.3%, respectively).

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“…The frit-based column immunoassay procedure was described in our previous publications [32,33]. First, polyethylene frits were degassed in ethanol by ultrasonication (15 min).…”

Section: Polyethylene Frit-based Immunoassay (Fbi) For On-site Detectionmentioning

confidence: 99%

Liposomes loaded with quantum dots for ultrasensitive on-site determination of aflatoxin M1 in milk products

et al. 2013

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“…The preparation and operation of polyethylene filter and immunoassay procedure was adopted from ref. 19 and was similar to the supplier's protocol. After degassing of the filters by placing them in ethanol and ultrasonication for 10 min the polyethylene filters were inserted into the column.…”

Section: Polyethylene Filter Preparationmentioning

confidence: 99%

Rapid immunochemical tests for qualitative and quantitative determination of T-2 and HT-2 toxins

et al. 2012

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Rapid immunotests were developed for qualitative and quantitative detection of T-2 and HT-2 toxins in cereal samples. Specific antibodies were immobilized either onto polyethylene filters or sepharose gel inside the transparent column. The tests combined the basic functions of purification, concentration and immunodetection of the target analytes through the enzymatic reaction of horseradish peroxidase (HRP). To make the test suitable for detection of both T-2 and HT-2 toxins, anti-T-2 toxin antibody was combined with HT-2 toxin-HRP conjugate. Matrix interference was eliminated by appropriate dilution of sample extracts with polyethylenglycol solution followed by filtration through a solid sorbent in a separate clean-up column. For the qualitative assay (visual detection) the cut-off level was 2 ng mL À1 of T-2 and HT-2 toxins, while the quantitative assay enabled the detection of 0.55 ng g À1 T-2 toxin and 1.7 ng g À1 HT-2 toxin in spiked wheat samples. Analysis of naturally contaminated wheat samples resulted in a good agreement between the developed immunotest and confirmatory LC-MS/ MS. The proposed immunotest is fast, easy-to-use and suitable for rapid screening of T-2 and HT-2 toxins in wheat.

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“…Abdulkadar 9 detected benzo(a)pyrene by GC-MS method in olive oils and the recovery and limitation were estimated as 88% and 0.5 mg kg À1 , respectively. In Beloglazova's work, 20 the detection limit of benzo(a)pyrene was 9 ng kg À1 using an immunochemical column test. The detection limit value of AFB1 was 15 ng kg À1 by ELISA method in Yu's work.…”

Section: Introductionmentioning

confidence: 99%

“…Some analytical techniques have been developed for the determination of benzo(a)pyrene and aatoxins in different samples, such as mainly gas chromatography-mass spectrometry (GC-MS), 9 thin layer chromatography (TLC), 10,11 enzymelinked immunosorbent assay (ELISA), [12][13][14][15] and high performance liquid chromatography (HPLC), 6,[16][17][18][19] etc. [20][21][22] However, GC-MS has the disadvantages of tedious pretreatment, large consumption of organic solvents and long operating time. TLC is a semi-quantitative analysis method, and it has shortcomings of false positive result.…”

Section: Introductionmentioning

confidence: 99%

Determination of benzo(a)pyrene and aflatoxins (B1, B2, G1, G2) in vegetable oil by GPC-HPLC-FLD

et al. 2014

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New approach to quantitative analysis of benzo[a]pyrene in food supplements by an immunochemical column test

Cited by 12 publications

References 23 publications

Liposomes loaded with quantum dots for ultrasensitive on-site determination of aflatoxin M1 in milk products

Liposomes loaded with quantum dots for ultrasensitive on-site determination of aflatoxin M1 in milk products

Rapid immunochemical tests for qualitative and quantitative determination of T-2 and HT-2 toxins

Determination of benzo(a)pyrene and aflatoxins (B1, B2, G1, G2) in vegetable oil by GPC-HPLC-FLD

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