Determination of benzo(a)pyrene and aflatoxins (B1, B2, G1, G2) in vegetable oil by GPC-HPLC-FLD

Wang, Hao; Zhao, Li; Yang, Hong-Mei; Guo, Qilei; Shi, Haiyang; Pan, Hongyan; Zhao, Liping; Qian, Cong

doi:10.1039/c3ay41955j

Cited by 18 publications

(4 citation statements)

References 22 publications

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“…This technique has excellent separation efficiency, can be automated, and can be applied to a broad variety of matrices and products. 18 GPC is a widely used technique in large-molecule samples due to its effective removal of interfering components and has been used in the detection of pesticide residues and antioxidants in eggs, 19 honey, 20 and pharmaceutical products. 21 The U.S. Environmental Protection Agency (EPA), the U.S. Food and Drug Administration (FDA), the American Association for Analytical Chemistry (AOAC) and the European Commission (EN) all consider GPC as an official method.…”

Section: Introductionmentioning

confidence: 99%

Determination of 10 photo-initiator residues in food plastic packaging by gel permeation chromatography extraction coupled with gas chromatography-mass spectrometry

et al. 2015

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An analytical method using gel permeation chromatography (GPC) coupled with gas chromatography-mass spectrometry (GC-MS) was established for the determination of 10 photo-initiatorsbenzophenone (BP), 2-methyl benzophenone (2-MBP), 1-hydroxycyclohexyl phenyl ketone (Irgacure 184), 3-methyl benzophenone (3-MBP), 4-methyl benzophenone (4-MBP), ethyl 4-dimethylaminobenzoate (EDB), 2,2-dimethoxy-2-phenylacetophenone (Irgacure 651), o-methyl-benzoyl benzoate (OMBB), 2-ethylhexyl-4-dimethylamino benzoate (EHA) and 2-isopropylthioxanthone (ITX)in plastic food packaging. The plastic samples were treated with ultrasound in cyclohexane/ethyl acetate (1 : 1, v/v). The extract was cleaned up by gel permeation chromatography. The final solution was subjected to GC-MS using an external standard method for quantitation in selected ion monitoring mode. The 10 target photo-initiators showed linear recovery relationships over the concentrations of 0.005 to 0.2 mg L À1 , with r 2 > 0.999. The recoveries from spiked samples (0.07 Â 10 À3 , 0.17 Â 10 À3 and 0.33 Â 10 À3 mg dm À2 of standard) for each of the 10 photo-initiators ranged from 77.0% to 120.6%, with relative standard deviations (RSDs) of 0.5-13.9%. The limits of detection (LOD) were from 0.03 Â 10 À3 mg dm À2 to 0.12 Â 10 À3 mg dm À2 . The developed extraction method is simple and yields a sufficiently pure test sample, while the analytical method meets the sensitivity and precision requirements for photo-initiator detection. This method could be routinely used to determine the levels of photo-initiator residues in plastic food packaging.

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Section: Introductionmentioning

confidence: 99%

Determination of 10 photo-initiator residues in food plastic packaging by gel permeation chromatography extraction coupled with gas chromatography-mass spectrometry

et al. 2015

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“…Many commonly used methods require sample preparation due to the different matrices of edible oil. Currently, a variety of methods are available for the extraction and isolation of mycotoxins from oil, such as liquid–liquid extraction or partitioning (LLE), frequently reported in the literature [ 101 , 102 , 103 , 104 , 105 ]; solid–phase extraction (SPE) [ 105 , 106 , 107 , 108 , 109 ]; immune affinity columns (IACs) [ 81 , 94 ]; IAC combined with dispersive liquid–liquid microextraction (DLLME) [ 91 ]; multifunctional cleanup columns [ 110 ]; the QuEChERS system [ 90 ]; gel permeation chromatography (GPC) [ 111 ]; immune assay extraction; and low-temperature cleanup (LTC) [ 112 , 113 , 114 , 115 ]. However, each method has its advantages and limitations.…”

Section: Methods For Detecting Afb 1 In Edible Oilmentioning

confidence: 99%

Recent Progress on Techniques in the Detection of Aflatoxin B1 in Edible Oil: A Mini Review

2022

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Contamination of agricultural products and foods by aflatoxin B1 (AFB1) is becoming a serious global problem, and the presence of AFB1 in edible oil is frequent and has become inevitable, especially in underdeveloped countries and regions. As AFB1 results from a possible degradation of aflatoxins and the interaction of the resulting toxic compound with food components, it could cause chronic disease or severe cancers, increasing morbidity and mortality. Therefore, rapid and reliable detection methods are essential for checking AFB1 occurrence in foodstuffs to ensure food safety. Recently, new biosensor technologies have become a research hotspot due to their characteristics of speed and accuracy. This review describes various technologies such as chromatographic and spectroscopic techniques, ELISA techniques, and biosensing techniques, along with their advantages and weaknesses, for AFB1 control in edible oil and provides new insight into AFB1 detection for future work. Although compared with other technologies, biosensor technology involves the cross integration of multiple technologies, such as spectral technology and new nano materials, and has great potential, some challenges regarding their stability, cost, etc., need further studies.

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“…Aatoxin B1 (AFB1)a highly toxic, low molecular weight metabolite of mold fungi, which poses a signicant threat to human and animal health [24][25][26] in the case of food and feedstock contaminationwas selected as the test compound. AFB1 is commonly detected by chromatographic methods [27][28][29] and various immunoassay methods dominated by microplate ELISA. [30][31][32] The given complex of superparamagnetic nanoparticles and antibodies against AFB1 was earlier used for the ELISA format which was carried out in volume of microplate wells.…”

Section: Introductionmentioning

confidence: 99%

Magnetic ELISA of aflatoxin B1 – pre-concentration without elution

et al. 2015

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Determination of benzo(a)pyrene and aflatoxins (B1, B2, G1, G2) in vegetable oil by GPC-HPLC-FLD

Cited by 18 publications

References 22 publications

Determination of 10 photo-initiator residues in food plastic packaging by gel permeation chromatography extraction coupled with gas chromatography-mass spectrometry

Determination of 10 photo-initiator residues in food plastic packaging by gel permeation chromatography extraction coupled with gas chromatography-mass spectrometry

Recent Progress on Techniques in the Detection of Aflatoxin B1 in Edible Oil: A Mini Review

Magnetic ELISA of aflatoxin B1 – pre-concentration without elution

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