New Approach in Rapid Viruses Detection and Its Implementation on a Chip

Abstract

Mortal viruses attack mankind last years and others can arrive early. The rapid detection of the viruses is very important to prevent against pandemic spread. The method of rapid detection is presented in this work. Techniques using micro-and nanotechnology are used for implementation of the method to lab on a chip. The paramagnetic particles are modified using biotechnology to make easy separation and electrochemical detection of viral nucleic acids. The method was approved for detection of viruses HIV and H5N1. Finally the device is designed with aim to prepare low-cost miniature system for mass screening in a case of pandemic.

Beads-Based Electrochemical Assay for the Detection of Influenza Hemagglutinin Labeled with CdTe Quantum Dots

“…Influenza type A can be subtyped by different variants of NA (1-9) and HA (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17), which differ from each other in virulence, host specificity and binding of HAs subtypes on human and/or avian glycan receptors [19][20][21][22][23][24]. Specific HA-glycan affinity has been exploited as the basis for isolation of various types of influenza viruses [25].…”

“…Combinations of HA (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17) and NA (1)(2)(3)(4)(5)(6)(7)(8)(9) subtypes affect the biological properties of influenza viruses, especially their host range and virulence. HA is responsible for the initiation of the infection and it binds sialic acid on the host cell surface.…”

“…As a detection system, electrochemical biosensors and bioassays have attracted considerable interest due to their high performance, miniaturized construction, and low costs [11][12][13][14][15]. In spite of the fact that quantum dots (QDs) and magnetic nanoparticles (MPs) are currently utilized, especially for in vivo and in vitro imaging, their application in the field of biosensing devices for influenza viruses could be also considered [16][17][18].…”