New anti-HIV aptamers based on tetra-end-linked DNA G-quadruplexes: effect of the base sequence on anti-HIV activity

D’Atri, Valentina; Oliviero, Giorgia; Amato, Jussara; Borbone, Nicola; D’Errico, Stefano; Mayol, Luciano; Piccialli, Vincenzo; Haider, Shozeb; Hoorelbeke, Bart; Balzarini, Jan; Piccialli, Gennaro

doi:10.1039/c2cc34399a

Cited by 31 publications

(29 citation statements)

References 20 publications

(24 reference statements)

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“…Later on, Koizumi et al synthesized a set of G-rich oligonucleotides and identified the hexadeosyribonucleotide d(TGGGAG), known as Hotoda’s sequence, [49,50]. Several authors have used the Hotoda’s sequence as a lead sequence to make a series of modifications at the 5′ and 3′ ends of the molecule or mutations in the sequence that allowed to find molecules with high anti-HIV activity [50,51,52,53,54,55,56]. …”

Section: Aptamers For Virus Diagnosis and Treatmentmentioning

confidence: 99%

Use of Aptamers as Diagnostics Tools and Antiviral Agents for Human Viruses

González

Martı́n

Fernández³

et al. 2016

Pharmaceuticals

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Appropriate diagnosis is the key factor for treatment of viral diseases. Time is the most important factor in rapidly developing and epidemiologically dangerous diseases, such as influenza, Ebola and SARS. Chronic viral diseases such as HIV-1 or HCV are asymptomatic or oligosymptomatic and the therapeutic success mainly depends on early detection of the infective agent. Over the last years, aptamer technology has been used in a wide range of diagnostic and therapeutic applications and, concretely, several strategies are currently being explored using aptamers against virus proteins. From a diagnostics point of view, aptamers are being designed as a bio-recognition element in diagnostic systems to detect viral proteins either in the blood (serum or plasma) or into infected cells. Another potential use of aptamers is for therapeutics of viral infections, interfering in the interaction between the virus and the host using aptamers targeting host-cell matrix receptors, or attacking the virus intracellularly, targeting proteins implicated in the viral replication cycle. In this paper, we review how aptamers working against viral proteins are discovered, with a focus on recent advances that improve the aptamers’ properties as a real tool for viral infection detection and treatment.

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Section: Aptamers For Virus Diagnosis and Treatmentmentioning

confidence: 99%

Use of Aptamers as Diagnostics Tools and Antiviral Agents for Human Viruses

González

Martı́n

Fernández³

et al. 2016

Pharmaceuticals

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“…[11,12] Further factorst hat contribute to the wide polymorphism of G-quadruplexes are the length and the base composition of the loops (when present), as well as the nature of the cationsu sed to stabilize the quadrupleh elix structure. [13][14][15] The biological implications of Gquadruplexes in cellular processes [16][17][18] and their use as promising drugs [19][20][21][22] or in drugs delivery [23] and diagnostics [23][24][25] are well known. In addition, G-quadruplexes possess greater conductivityt han DNA duplexes, thus suggesting their use for the obtainment of electronic nano-biomaterials and nanodevices.…”

Section: Introductionmentioning

confidence: 99%

Self‐Assembly of G‐Rich Oligonucleotides Incorporating a 3′–3′ Inversion of Polarity Site: A New Route Towards G‐Wire DNA Nanostructures

et al. 2017

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Obtaining DNA nanostructures with potential applications in drug discovery, diagnostics, and electronics in a simple and affordable way represents one of the hottest topics in nanotechnological and medical sciences. Herein, we report a novel strategy to obtain structurally homogeneous DNA G‐wire nanostructures of known length, starting from the short unmodified G‐rich oligonucleotide d(5′‐CGGT‐3′–3′‐GGC‐5′) (1) incorporating a 3’–3′ inversion of polarity site. The reported approach allowed us to obtain long G‐wire assemblies through 5′–5′ π–π stacking interactions in between the tetramolecular G‐quadruplex building blocks that form when 1 is annealed in the presence of potassium ions. Our results expand the repertoire of synthetic methodologies to obtain new tailored DNA G‐wire nanostructures.

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“…A series of hexadeoxyribonucleotides, carrying various modifications at either the 5′ or the 3′ ends, based on the so called d(TGGGAG) Hotoda's sequence, was also synthesized and evaluated in vitro for their anti-HIV activity, proving to be potent gp120 inhibitors, with IC 50 values from sub-micromolar to nanomolar concentrations [101][102][103][104].…”

Section: Antiviral G4-aptamersmentioning

confidence: 99%

G-quadruplex-based aptamers against protein targets in therapy and diagnostics

Platella

Riccardi

Montesarchio

et al. 2017

Biochimica et Biophysica Acta (BBA) - General Subjects

132

116

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Nucleic acid aptamers are single-stranded DNA or RNA molecules identified to recognize with high affinity specific targets including proteins, small molecules, ions, whole cells and even entire organisms, such as viruses or bacteria. They can be identified from combinatorial libraries of DNA or RNA oligonucleotides by SELEX technology, an in vitro iterative selection procedure consisting of binding (capture), partitioning and amplification steps. Remarkably, many of the aptamers selected against biologically relevant protein targets are G-rich sequences that can fold into stable G-quadruplex (G4) structures. Aiming at disseminating novel inspiring ideas within the scientific community in the field of G4-structures, the emphasis of this review is placed on: 1) recent advancements in SELEX technology for the efficient and rapid identification of new candidate aptamers (introduction of microfluidic systems and next generation sequencing); 2) recurrence of G4 structures in aptamers selected by SELEX against biologically relevant protein targets; 3) discovery of several G4-forming motifs in important regulatory regions of the human or viral genome bound by endogenous proteins, which per se can result into potential aptamers; 4) an updated overview of G4-based aptamers with therapeutic potential and 5) a discussion on the most attractive G4-based aptamers for diagnostic applications. This article is part of a Special Issue entitled "G-quadruplex" Guest Editor: Dr. Concetta Giancola and Dr. Daniela Montesarchio.

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New anti-HIV aptamers based on tetra-end-linked DNA G-quadruplexes: effect of the base sequence on anti-HIV activity

Cited by 31 publications

References 20 publications

Use of Aptamers as Diagnostics Tools and Antiviral Agents for Human Viruses

Use of Aptamers as Diagnostics Tools and Antiviral Agents for Human Viruses

Self‐Assembly of G‐Rich Oligonucleotides Incorporating a 3′–3′ Inversion of Polarity Site: A New Route Towards G‐Wire DNA Nanostructures

G-quadruplex-based aptamers against protein targets in therapy and diagnostics

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