New Andean source of resistance to anthracnose and angular leaf spot: Fine-mapping of disease-resistance genes in California Dark Red Kidney common bean cultivar

Abstract

Anthracnose (ANT) and angular leaf spot (ALS) caused by Colletotrichum lindemuthianum and Pseudocercospora griseola, respectively, are devastating diseases of common bean around the world. Therefore, breeders are constantly searching for new genes with broadspectrum resistance against ANT and ALS. This study aimed to characterize the genetic resistance of California Dark Red Kidney (CDRK) to C. lindemuthianum races 73, 2047, and 3481 and P. griseola race 63-39 through inheritance, allelism testing, and molecular analyses. Genetic analysis of response to ANT and ALS in recombinant inbred lines (RILs) from a CDRK × Yolano cross (CY) showed that the resistance of CDRK cultivar is conferred by a single dominant loci, which we named CoPv01 CDRK /PhgPv01 CDRK. Allelism tests performed with race 3481showed that the resistance gene in CDRK is independent of the Co-1 and Co-AC. We conducted co-segregation analysis in genotypes of 110 CY RILs and phenotypes of the RILs in response to different races of the ANT and ALS pathogens. The results revealed that CoPv01 CDRK and PhgPv01 CDRK are coinherited, conferring resistance to all races. Genetic mapping of the CY population placed the CoPv01 CDRK /PhgPv01 CDRK loci in a 245 Kb genomic region at the end of Pv01. By genotyping 19 RILs from the CY population using three additional markers, we fine-mapped the CoPv01 CDRK /PhgPv01 CDRK loci to a smaller genomic region of 33 Kb. This 33 Kb region harbors five predicted genes based on the common bean reference genome. These results can be applied in breeding programs to develop bean cultivars with ANT and ALS resistance using marker-assisted selection.

Integration of anthracnose resistance loci and RLK and NBS‐LRR‐encoding genes in the Phaseolus vulgaris L. genome

“…Additionally, the predicted gene Phvul.001G245300 that encodes for a putative LRR‐protein kinase (LRR‐kinase) was detected at approximately 66 kb upstream of the marker locus CV542014. Similarly, another putative gene, Phvul.001G246800 , which encodes an LRR‐kinase, was found only 26 kb downstream of the marker ss715645248 (Gonçalves‐Vidigal et al., 2020).…”

North-Western Himalayan Common Beans: Population Structure and Mapping of Quantitative Anthracnose Resistance Through Genome Wide Association Study

“…Hence, in recent years, use of GWAS for mapping economically important QTLs in agricultural crops has increased and P. vulgaris is not behind. Many QTLs having economic importance in common bean have been mapped using a GWAS approach ( Moghaddam et al, 2016 ; Wu et al, 2017 ; Kelly and Bornowski, 2018 ; Kamfwa et al, 2019 ; Bisneta and Gonçalves-Vidigal, 2020 ; Bornowski et al, 2020 ; Gonçalves-Vidigal et al, 2020 ; Mungalu et al, 2020 ). In the present study, significant differences observed in NW Indian bean genotypes led to the identification of SNPs associated with the anthracnose resistance.…”

Genome‐wide association study of resistance to anthracnose and angular leaf spot in Brazilian Mesoamerican and Andean common bean cultivars

“…The monogenic and dominant Mesoamerican genes include Co‐2 , Co‐3 (and its alleles Co‐3 2 , Co‐3 3 , Co‐3 4 , Co‐3 5 ), Co‐4 (and its alleles Co‐4 2 , Co‐4 3 ), Co‐5 (and its allele Co‐5 2 ), Co‐6 , Co‐11 , Co‐16 , Co‐17 , Co‐u , and Co‐v (Coimbra‐Gonçalves et al., 2016; Gonçalves‐Vidigal, Silva, Vidigal Filho, Gonela, & Kvitschal, 2007; Kelly & Vallejo, 2004; Méndez‐Vigo, Rodriguez‐Suarez, Paneda, Ferreira, & Giraldez, 2005; Meziadi et al., 2016). The ANT resistance genes of Andean origin include Co‐1 (and its alleles Co‐1 2 , Co‐1 3 , Co‐1 4 , Co‐1 5 , Co‐1 HY ), Co‐12 , Co‐13 , Co‐14 , Co‐15 , Co‐Pa , Co‐AC , CoPv01 CDRK , Co‐x , Co‐w , Co‐y , and Co‐z (Chen et al., 2017; Gonçalves‐Vidigal & Kelly, 2006; Gonçalves‐Vidigal et al., 2011, 2012, 2013, 2020; Gonçalves‐Vidigal, Lacanallo, & Vidigal Filho, 2008; Gonçalves‐Vidigal, Vidigal Filho, Medeiros, & Pastor‐Corrales, 2009; Kelly & Vallejo, 2004; Lima et al, 2017; Gilio et al, 2017; Meziadi et al., 2016; Richard et al., 2014). Monogenic dominant loci conferring ALS resistance associated with the Andean or Mesoamerican gene pools have also been reported.…”

“…The resistance locus Phg‐2 , which present in the Mesoamerican common bean cultivar Mexico 54, is tagged to the SN02 and OPE04 markers at distances of 5.9 and 11.8 cM, respectively, on chromosome Pv08 (Sartorato et al., 2000). The Mesoamerican resistance locus Phg‐3 , present in in the Mesoamerican common bean cultivar Ouro Negro is linked to the g2303 marker at a distance of 0.0 cM on Pv04 (Gonçalves‐Vidigal et al., 2013). Two monogenic dominant resistance loci were also identified in the Mesoamerican cultivar G10909 (Mahuku et al., 2011).…”