New and Sensitive Method for the Spectrophotometric Determination of Certain Tricyclic Antidepressants

Nagaraja, Padmarajaiah; Silwadi, Mohammed Fawaz; Syed, Akheel Ahmed

doi:10.1007/s006040070009

Cited by 14 publications

(8 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several techniques have been employed to determine the TCAs in biological samples and pharmaceuticals, including spectrophotometry [3], spectrofluorimetry [4], RIA [5], GC [6], and HPLC [7,8]. Recently, CE also has become a popular technique for the determination of TCAs [9 -22] because of its high efficiency and versatility, very low sample and reagent consumption, low cost, and minimization of environmental pollution.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoretic separation of tricyclic antidepressants using a polymer‐coated capillary and β‐cyclodextrin as an electrolyte additive

Lin

Whang²

2008

J of Separation Science

View full text Add to dashboard Cite

A new method for the CE separation of nine tricyclic antidepressants (TCAs), viz. amitriptyline, clomipramine, desipramine, doxepin, fluphenazine, imipramine, nortriptyline, promazine, and thioridazine, is described. The capillary was statically coated with a layer of poly(N,N-dimethylacrylamide) (PDMA) to suppress the EOF, and beta-CD was used as an additive in the BGE solution. The optimal resolution of nine TCAs was obtained by using a 1% v/v PDMA-coated capillary and a BGE solution of 50 mM sodium phosphate buffer (pH 3.0) containing 0.5 mM beta-CD. Efficiencies were typically >10(5 )plates/m. Complete separation of nine TCAs could be achieved in about 28 min; the two diastereomers of doxepin and the two enantiomers of thioridazine could also be separated. The RSD values of migration time and peak area of the TCAs were in the ranges 0.5-0.8 and 3.3-4.9% (n = 10), respectively. In combination with a suitable sample clean-up technique, such as hollow fiber-based liquid phase microextraction (HF-LPME), the polymer-coated capillary can be employed for the CE-UV analysis of TCAs in human plasma.

show abstract

Section: Introductionmentioning

confidence: 99%

Capillary electrophoretic separation of tricyclic antidepressants using a polymer‐coated capillary and β‐cyclodextrin as an electrolyte additive

Lin

Whang²

2008

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…Agentes oxidantes, como Fe (III) e metavanadato de amônio, podem ser utilizados como precursores espectrofotométricos para determinação de diferentes ADTs, embora os métodos propostos requeiram meios ácidos ou longo tempo de reação. [4][5][6] A formação de complexos é outra alternativa utilizada para a quantificação de ADTs por espectrofotometria. Podem estar baseados na reação com moléculas orgânicas ou com metais de transição.…”

Section: Introductionunclassified

“…Além disso, os meios reacionais são . 11 Dentre os exemplos de moléculas orgânicas utilizadas na formação de pares iônicos, encontra-se o azul de timol, o cromo azurol S, verde de bromocresol, alaranjado de metila e alizarina S. 14,[16][17][18][19][20] Embora não se utilizem reagentes em elevada concentração, faz-se necessário o uso de solventes orgânicos para extração, condição que implica em maior risco ao operador.…”

Section: Introductionunclassified

Determinação turbidimétrica do antidepressivo amitriptilina em sistema fia explorando a formação do par iônico com lauril sulfato de sódio

Silveira¹,

Tarley²

2008

Quím. Nova

View full text Add to dashboard Cite

Recebido em 11/8/07; aceito em 4/4/08; publicado na web em 4/9/08 TURBIDIMETRIC DETERMINATION OF ANTIDEPRESSANT AMITRIPTYLINE IN FIA SYSTEM EXPLOITING THE ION-PAIR FORMATION WITH SODIUM LAURYL SULPHATE. The present work purposes the development of an analytical method for amitriptyline determination in pharmaceutical formulations using FIA system. It was based on interaction of amitriplyline with sodium lauryl sulphate in acid medium (pH 2.5) resulting in the ion-pair formation turbidimetrically detected at 410 nm. The fitting regression equation for range curve from 2.0 x 10 -3 up to 3.2 x 10 -3 mol L -1 was found to be analytical signal = -2.7417 + 0.1538 [amitriptyline] (r = 0.99991) with a detection limit of 1.8 x 10 -3 mol L -1. The precision assessed as relative standard deviation (n = 10) was found to be 2.40 and 1.94%, for the respective concentration of amitriplyline 2.0 x 10 -3 and 3.2 x 10 -3 mol L -1 and the sample throughout was 60 h Quando usados em doses excessivas, os ADTs podem ser nocivos à saúde, levando até a morte do paciente. Mesmo em doses terapêuticas, há a ocorrência de diversos efeitos colaterais, tais como sonolência e sedação excessiva; confusão mental, secura da boca e visão turva. Sobre o aparelho cardiovascular há o aparecimento de taquicardia e modificações da pressão sanguínea quando administrados em superdosagem.1 Por isso, o controle do teor do medicamento administrado é importante para o sucesso do tratamento e minimização dos efeitos colaterais.A farmacopéia americana recomenda a quantificação por métodos cromatográficos ou titulação com ácido perclórico.2 Outros métodos cromatográficos também são relatados na literatura para a quantificação de AMT em plasma humano. 3Para análise de fármacos, os métodos espectrofotométricos têm sido bastante empregados. Várias estratégias podem ser utilizadas para essa análise. Agentes oxidantes, como Fe (III) e metavanadato de amônio, podem ser utilizados como precursores espectrofotométricos para determinação de diferentes ADTs, embora os métodos propostos requeiram meios ácidos ou longo tempo de reação. [4][5][6] A formação de complexos é outra alternativa utilizada para a quantificação de ADTs por espectrofotometria. Podem estar baseados na reação com moléculas orgânicas ou com metais de transição. A quantificação de vários ADTs com cloranil é relatada por Ibrahim et al.. 7 Abdel-Salam e colaboradores propõem o uso do reagente 2,3 -dicloro 5, 6 diciano -p -benzoquinona (DDQ) para a avaliação do teor de ADTs, dentre eles a AMT, em formulações farmacêuticas. Entretanto, as reações com formação desses complexos ocorrem em meio orgânico, tais como uma mistura de dioxano-etanol, acetonitrila, acetona ou dicloroetano. Além disso, as reações são lentas e requerem aquecimento. A quantificação do complexo de transferência de carga AMT-molibdato de amônio em H 2 SO 4 concentrado é descrita por Aman et al.. 10O emprego de pares iônicos, que consistem na interação eletrostática entre o fármaco, protonado, e um contra-íon, carregado negativamente,...

show abstract

“…The reported chromatographic techniques [7][8][9][10][11][12][13] require expensive experimental set-up, requires sophisticated instrumentation [15] and involvement of scrupulous experimental conditions which are not affordable in every laboratory for routine analysis; Whereas the reported spectrophotometric methods were associated with some major drawbacks such as, lesser sensitivity [16][17][23][24][25][26], tedious extraction procedures [16][17]26], maintenance of strict experimental conditions [16,17,21] and time consuming [18,20,27]. For these reasons, the development of a new, simple, rapid, selective, sensitive and inexpensive spectrophotometric method that overcomes the drawbacks of the existing methodologies was very essential.…”

Section: Introductionmentioning

confidence: 99%

“…These methods include chromatographic techniques like HPLC-DAD method using 7,7,8,8-tetracyano-quinodimethane (TCNQ) as a derivatisation agent [7], HPLC with liquid-liquid micro extraction technique [8], RP-HPLC and HPTLC [9] GC [10], GC-MS [11-13,], electrogenerated chemiluminescence [14,], voltammetry [15], spectrofluorimetry [16] and spectrophotometry [17][18][19][20][21][22][23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Antidepressant Drugs: Highly Sensitive and Validated Spectrophotometric Technique

Deepakumari¹,

Prashanth

Revanasiddappa

2014

J. Chil. Chem. Soc.

View full text Add to dashboard Cite

A simple, rapid, selective and highly sensitive spectrophotometric method is described for the quantitative determination of the tricyclic antidepressant drugs, desipramine hydrochloride (DPH), clomipramine hydrochloride (CPH) and imipramine hydrochloride (IMH) in pure and in pharmaceutical preparations. The proposed method is based on the bromination of above drugs with known excess of bromine. The unreacted bromine is determined based on its ability to bleach the dye Eiochrome blue black R quantitatively at 530 nm for all the three drugs obeying Beer's law in the range, 0.0 -8, 0.0 -10 and 0.0 -9.0 µg ml -1 for DPH, CPH and IMH, respectively. The molar absorptivity values were found to be 1.61 × 10 4 , 1.62 × 10 4 and1.57 × 10 4 l mol -1 cm -1 , respectively with the corresponding Sandell's sensitivity values 0.0187, 0.0216 and 0.0202 µg cm -2 . The limits of detection and (LOD) and quantification (LOQ) are also reported for the developed method. Intra-and inter-day precision and accuracy of the method were established according to the current ICH guidelines. Applications of the procedure to the analysis of various pharmaceutical preparations gave reproducible and accurate results. Further, the validity of the procedure was confirmed by applying the standard addition technique and the results were evaluated in terms of Student's t-test and variance ratio F-test to find out the significance of proposed method over the reference method.

show abstract

New and Sensitive Method for the Spectrophotometric Determination of Certain Tricyclic Antidepressants

Cited by 14 publications

References 6 publications

Capillary electrophoretic separation of tricyclic antidepressants using a polymer‐coated capillary and β‐cyclodextrin as an electrolyte additive

Capillary electrophoretic separation of tricyclic antidepressants using a polymer‐coated capillary and β‐cyclodextrin as an electrolyte additive

Determinação turbidimétrica do antidepressivo amitriptilina em sistema fia explorando a formação do par iônico com lauril sulfato de sódio

Antidepressant Drugs: Highly Sensitive and Validated Spectrophotometric Technique

Contact Info

Product

Resources

About