Neutrophil, TLR2, and TLR4 expression in newborns at risk of sepsis

Yunanto, Ari; Endharti, Agustina Tri; Widodo, Aris Puji

doi:10.14238/pi53.3.2013.02

Cited by 2 publications

(2 citation statements)

References 22 publications

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“…The incomplete understanding of mechanisms underlying organ damage and immune defense during septic insult hinder the development of more comprehensive management ( 23 ). Accumulating evidence suggests that autophagic activity is increased during the initial phase of sepsis ( 24 , 25 ) and LPS, a key bacterial product, may initiate autophagy through toll-like receptor 2 and toll-like receptor 4 activation ( 26 , 27 ). Two studies indicated a protective role for such a response ( 24 , 28 ); however, the detailed underlying mechanism requires further investigation.…”

Section: Discussionmentioning

confidence: 99%

miR‑23a downregulation modulates the inflammatory response by targeting ATG12‑mediated autophagy

et al. 2018

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Autophagy, part of the innate immune defense mechanisms, is activated during the initial phase of septic insult. Previous studies indicated that micro (mi)RNAs are additionally involved in the host response to sepsis; however, the association between miRNAs and autophagy during this process is not fully understood. To study the role of miRNA (miR)-23a in autophagy initiated by sepsis, macrophages treated with lipopolysaccharides, in addition to blood samples from patients, were evaluated for miR-23a expression levels. Cell viability, inflammatory mediators and autophagic markers were investigated following overexpression or inhibition of miR-23a. The results suggested that miR-23a was suppressed subsequent to septic insult, promoting autophagy and suppressing a hyper inflammatory response, leading to enhanced cell viability. A luciferase assay and western blot analysis confirmed ubiquitin-like protein ATG12 to be the target of miR-23a. The present study revealed that the downregulation of miR-23a regulates an inflammatory response during septic insult via autophagy promotion.

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Section: Discussionmentioning

confidence: 99%

miR‑23a downregulation modulates the inflammatory response by targeting ATG12‑mediated autophagy

et al. 2018

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“…Several approaches to caries prevention have been attempted including access to dental health education (specifically, the appropriate method of brushing the teeth), the provision of topical fluoride, and vaccination. 20 However, to date, none of these has produced the desired outcome. This study aims to identify the risk factor of dental caries and the innate immunity mechanism which can protect against or prevent these.…”

Section: Introductionmentioning

confidence: 99%

CD89/CD35 Expression Ratio in Salivary Neutrophil as an Early Detection Marker for Severe Early Childhood Caries

et al. 2020

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Abstract Objectives To analyze CD35/CD89 expression ratio on the surface of neutrophils as an early detection marker for S-ECC. Materials and Methods Saliva was collected from 4- to 6-year-old kindergarten students. Salivary neutrophils were obtained by instructing the subjects to rinse their mouth with 1 mL of sterile 1.5% NaCl for 30 seconds before expectorating it into a sterile glass. The expression of CFSE+CD35+ and CFSE+CD89+was measured and analyzed using flow cytometry. Results The expression of CFSE+CD89+ in the caries-free group (2.46 ± 0.39) was significantly lower than that in the S-ECC group (3.41 ± 1.11), with a p-value of 0.0001, while the expression of CFSE+CD35+ in the caries-free group was (2.35 ± 0.56) compared with (1.54 ± 0.35) (p = 0.0001) in the S-ECC group. Conclusions The expression ratio of CFSE+CD89+ and CFSE+CD35+constitutes a marker for S-ECC.

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Neutrophil, TLR2, and TLR4 expression in newborns at risk of sepsis

Abstract: Background There is increasing evidence that toll-like receptors

Cited by 2 publications

References 22 publications

miR‑23a downregulation modulates the inflammatory response by targeting ATG12‑mediated autophagy

miR‑23a downregulation modulates the inflammatory response by targeting ATG12‑mediated autophagy

CD89/CD35 Expression Ratio in Salivary Neutrophil as an Early Detection Marker for Severe Early Childhood Caries

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