Neutrophil extracellular traps formation by bacteria causing endometritis in the mare

Rebordão, Maria Rosa; Carneiro, C.; Alexandre‐Pires, Graça; Brito, Patrícia H.; Pereira, Catarina; Nunes, Teresa; Galvão, António; Leitão, Alexandre; Vilela, C. L.; Ferreira‐Dias, Graça

doi:10.1016/j.jri.2014.08.003

Cited by 59 publications

(48 citation statements)

References 42 publications

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“…The nuclear lysis seen cytologically in these airway samples and classified as degenerate neutrophilia has a strong cytological resemblance to neutrophil extracellular traps (NETs, netosis), a mechanism by which neutrophils actively degenerate and release chromatin and associated antimicrobial molecules to trap and kill microbes [21,22]. Netosis has been documented in most species, including horses [23,24], and this active mechanism of cell death warrants further investigation in potential roles in pathogenesis, diagnosis and therapeutic targets for mild-moderate asthma with an associated bacterial component.…”

Section: Discussionmentioning

confidence: 91%

Association of neutrophil morphology with bacterial isolates in equine tracheal wash samples

Jocelyn

Wylie

Lean

et al. 2018

Equine Veterinary Journal

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Cytological evaluation of tracheal washes should include cytomorphological features of the neutrophil response. The presence of degenerate neutrophils, especially in young horses, indicates added value of culture and sensitivity for antimicrobial therapeutics. The absence of degenerate changes, in combination with clinical factors, can help support diagnosis of a nonseptic cause of airway neutrophilia.

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Section: Discussionmentioning

confidence: 91%

Association of neutrophil morphology with bacterial isolates in equine tracheal wash samples

Jocelyn

Wylie

Lean

et al. 2018

Equine Veterinary Journal

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“…Neutrophils in blood circulation could firmly adhere to the endothelium cell barrier, cross it, and are recruited into sites of inflammation in different pathogen-infected tissues, which is the first line of innate immune defense against pathogens [33]. Recently, study also showed the infiltration of neutrophils into the endometrium of mice with lipopolysaccharides (LPS)-induced endometritis [34].…”

Section: Discussionmentioning

confidence: 99%

The anti-inflammatory effect of Andrographis paniculata (Burm. f.) Nees on pelvic inflammatory disease in rats through down-regulation of the NF-κB pathway

Zou

Xiao

Wen

et al. 2016

BMC Complement Altern Med

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Background Andrographis paniculata (Burm. f.) Nees (APN), a principal constituent of a famous traditional Chinese medicine Fukeqianjin tablet which is used for the treatment of pelvic inflammatory disease (PID), has been reported to have anti-inflammatory effect in vitro. However, whether it has pharmacological effect on PID in vivo is unclear. Therefore, the aim of this study is to test the anti-inflammatory effect of APN and illuminate a potential mechanism.MethodsThirty-six female specific pathogen-free SD rats were randomly divided into control group, PID group, APN1 group, APN2 group, APN3 group and prednisone group. Pathogen-induced PID rats were constructed. The APN1, APN2 and APN3 group rats were orally administrated with APN extract at different levels. The prednisone group rats were administrated with prednisone. Eight days after the first infection, the histological examination of upper genital tract was carried out, and enzyme-linked immunosorbent assay (ELISA) was carried out using homogenate of the uterus and fallopian tube. Furthermore, immunohistochemical evaluations of NF-κB p65 and IκB-α in uterus was conducted.ResultsAPN obviously suppressed the infiltrations of neutrophils and lymphocytes, and it could significantly reduce the excessive production of cytokines and chemokines including IL-1β, IL-6, CXCL-1, MCP-1 and RANTES in a dose-dependent manner. Furthermore, APN could block the pathogen-induced activation of NF-κB pathway.ConclusionAPN showed potent anti-inflammatory effect on pathogen-induced PID in rats, with a potential mechanism of inhibiting the NF-κB signal pathway.

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“…We have shown that equine PMN are able to form NETs in vitro, when in presence of bacteria causing endometritis in the mare, and also in the uteri of mares with endometritis by E .coli or/and S. zooepidemicus (Rebordão et al 2014). When we tested in vitro the role of NETs components on mare endometrium, a decrease in PGE2 production and a rise in mRNA type 1 collagen level were observed, which may lead to fibrosis establishment.…”

Section: Ecbolic Hormones and Diseasementioning

confidence: 93%

Dual effect of hormones on mare reproductive physiology and dysfunction

Rebordão¹,

Amaral²,

Galvão³

et al. 2016

PHK

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Summary: In the last decades, classical and molecular biology approaches have greatly contributed for building up sound knowledge on the mechanisms regulating reproductive processes in the mare. Different perspectives on the role of hormones should be addressed and thus considered by both researchers and clinicians. While oxytocin is largely used as a post-insemination method to improve fertility or prevent endometritis, it may also be considered to prolong the luteal phase. Prostaglandin E2 (PGE2), known to sustain luteostasis, has also been related to inflammatory processes in sub-clinical endometritis in mares. In contrast, as other ecbolic agents, PGF2a besides being luteolytic and currently used for estrous cycle shortening and endometritis control, might also play a role in fibrogenesis in mare endometrium. Hormones, like oxytocin, ovarian steroids, prostaglandins (PG), as well as enzyme profiles involved in endocrine pathways may act in a dissimilar fashion, depending on the estrous cycle phase and on the physiologic or pathologic condition.Keywords: mare / reproduction / oxytocin / prostaglandin / endometrium / fibrosis / ovarian steroids Doppeleffekt von Hormonen auf die Reproduktionsphysiologie und -störung bei der StuteIn den letzten Jahrzehnten haben die klassischen und molekularbiologische Methoden in hohen Maße für die Entwicklung fundierten Wissens zu den Regelmechanismen reproduktiver Prozesse bei der Stute beigetragen. Es sollten die unterschiedliche Sichtweisen auf die Rolle der Hormone berücksichtigt werden, sowohl von Forschern als auch Klinikern. Während Oxytocin überwiegend als Verfahren nach der Insemination verwendet wird, um die Fruchtbarkeit zu verbessern oder eine Endometritis zu verhindern, kann es auch eingesetzt werden, um die Gelbkörperphase zu verlängern. Prostaglandin E2 (PGE2) ist bekannt, die Luteostase zu erhalten, wird aber auch mit entzündlichen Prozessen bei der equinen subklinische Endometritis in Verbindung gebracht. Im Gegensatz dazu könnte PGF2a, wie andere ekbolische Mittel, abgesehen davon, dass es luteolytisch wirkt und derzeit benutzt wird, den Ovarialzyklus zu verkürzen und eine Endometritis zu kontrollieren, auch eine Rolle bei der Fibrogenese im equinen Endometrium spielen. Hormone wie Oxytocin, Ovarialsteroide, Prostaglandine (PG), als auch Enzymprofile, die an endokrinen Regelkreisen beteiligt sind, können in einer unterschiedlichen Art und Weise wirken, abhängig von der Zyklusphase und dem physiologischen oder pathologischen Zustand.

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Neutrophil extracellular traps formation by bacteria causing endometritis in the mare

Cited by 59 publications

References 42 publications

Association of neutrophil morphology with bacterial isolates in equine tracheal wash samples

Association of neutrophil morphology with bacterial isolates in equine tracheal wash samples

The anti-inflammatory effect of Andrographis paniculata (Burm. f.) Nees on pelvic inflammatory disease in rats through down-regulation of the NF-κB pathway

Dual effect of hormones on mare reproductive physiology and dysfunction

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