Neutrophil extracellular traps and dsDNA predict outcomes among patients with ST-elevation myocardial infarction

Abstract

Neutrophil extracellular traps (NETs) which have a potential role in noninfectious diseases, may play an important role in patients with acute coronary syndrome. The goal of this study was to investigate the association of NETs and in-hospital major adverse cardiac events among patients with ST-segment elevation myocardial infarction (STEMI). Using immunofluorescence staining, ELISA, and fluorescent enzyme standard instrument, we assessed NETs and NETs-related factors. Multivariate analyses were performed after univariate analyses to investigate which variables were independently associated with major adverse cardiac events. Compared with peripheral arteries, we observed neutrophils obtained from infarct-related artery (IRA) releasing NETs. The dsDNA levels, NET-specific marker myeloperoxidase/deoxyribonucleic acid (MPO/DNA) complexes and NETs-related factor tissue factor were significantly higher in coronary plasma samples. Multivariate analysis that white cell counts and coronary dsDNA were independently associated with in-hospital major adverse cardiac events. ROC curve for coronary dsDNA showed sensitivity of 78.0% and specificity of 53% for the cut-off value of 0.39ug/ml. Conclusion, these results provide evidences indicating NETs were associated with STIM, and occurrence of adverse cardiac events.

Neutrophil Extracellular Traps and Cardiovascular Diseases: An Update

“…Another study pointed out the prognostic role of NETs. In a cohort of patients with recent STEMI, higher levels of coronary dsDNA were found to independently predict in-hospital major adverse CV events (MACEs) [33]. These results, however, confirmed those previously reported by Borissoff et al, who in 2013 described for the first time a positive association between high levels of circulating dsDNA, nucleosomes, and MPO-DNA complexes and the occurrence of MACEs [29].…”

Double-Stranded DNA and NETs Components in Relation to Clinical Outcome After ST-Elevation Myocardial Infarction

“…However, dsDNA in the coronary and peripheral circulation is reportedly highly correlated 17 . Interestingly, it was also recently demonstrated in STEMI patients that even though both dsDNA and the more specific marker MPO-DNA were higher in the coronary than peripheral circulation, a considerable overlap was detected for dsDNA levels but not MPO-DNA levels, suggesting that MPO-DNA measured peripherally is a poorer reflection of coronary NETosis 18 . Thus, it appears that dsDNA and the more specific NETs markers measured in the peripheral circulation differentially represent the local thrombo-inflammatory milieu in the coronary circulation with adhesive stationary neutrophils.…”

Neutrophil extracellular traps and echogenicity of carotid atherosclerotic plaques

“…Langseth et al установили, что увеличение содержания двухцепочечной ДНК (маркер НВЛ) в сыворотке крови пациентов со стабильной ИБС независимо ассоциировалось с риском развития неблагоприятных сердечно-сосудистых событий в течение двух лет наблюдения [23]. Также была зарегистрирована прогностическая значимость повышенной генерации НВЛ циркулирующими нейтрофилами у пациентов с острым коронарным синдромом [24]. С другой стороны, в представленном исследовании количество НВЛ, формируемых циркулирующими нейтрофилами, у пациентов с каротидным атеросклерозом значимо не отличалось от пациентов с интактными сонными артериями.…”