Neutrophil Depletion Attenuates Muscle Injury after Exhaustive Exercise

Kawanishi, Noriaki; Mizokami, Tsubasa; Niihara, Hiroyuki; Yada, Keigo; Suzuki, Katsuhiko

doi:10.1249/mss.0000000000000980

Cited by 57 publications

(62 citation statements)

References 35 publications

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“…We demonstrated that neutrophils firstly migrate to the damaged muscle, attract macrophages, and induce inflammatory mediators and cytokines [12]. Although curcumin is known to protect against ischemia/reperfusion injury in rat skeletal muscle [22], we demonstrated exercise-induced oxidative stress was attenuated by prior curcumin ingestion in vivo in humans [23].…”

Section: Related and Supplementary Findingsmentioning

confidence: 81%

“…Although neutrophils migrate into damaged tissues following injury, infiltration of neutrophils into damaged muscles within several hours after exercise has also been demonstrated [2]. In the exercise and sport science fields, we have reported that exhaustive exercise facilitates neutrophil activity, suggesting their involvement with muscle damage [3][4][5][6][7][8][9][10][11][12]. However, endogenous antioxidant capacity also increases following exercise, which can partially attenuate neutrophil activation and oxidative stress [13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

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Exhaustive Exercise-Induced Neutrophil-Associated Tissue Damage and Possibility of its Prevention

Suzuki¹

2017

J Nanomedine Biotherapeutic Discov

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Neutrophils and inflammatory cytokines that accumulate in tissues often cause organ damage/dysfunctions. Herein, background information on some research findings of exertional effects on systemic inflammation centered on neutrophils is introduced. Furthermore, a functional activity measurement system of neutrophils including cell migration and reactive oxygen species (ROS) production, and preventive countermeasures against neutrophilinduced pathogenesis (e.g., polyphenols) are described.

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Section: Related and Supplementary Findingsmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

Exhaustive Exercise-Induced Neutrophil-Associated Tissue Damage and Possibility of its Prevention

Suzuki¹

2017

J Nanomedine Biotherapeutic Discov

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“…Exercise-induced muscle damage (EIMD), which is one of the reasons for DOMS, is caused by not only mechanical factors, but also non-mechanical factors [3]. One possible mechanism causing muscle damage is the invasion of leukocytes, especially phagocytes, including neutrophils and macrophages, into skeletal muscle [4,5,6]. Phagocytes infiltrating muscle tissue play essential roles in tissue repair, such as protein degradation and removal of cellular debris, whereas activated phagocytes release pro-inflammatory cytokines and reactive oxygen species (ROS), and induce inflammation as well as myofiber membrane lysis [4,7].…”

Section: Introductionmentioning

confidence: 99%

“…One possible mechanism causing muscle damage is the invasion of leukocytes, especially phagocytes, including neutrophils and macrophages, into skeletal muscle [4,5,6]. Phagocytes infiltrating muscle tissue play essential roles in tissue repair, such as protein degradation and removal of cellular debris, whereas activated phagocytes release pro-inflammatory cytokines and reactive oxygen species (ROS), and induce inflammation as well as myofiber membrane lysis [4,7]. In addition, ROS derived from phagocytes enhance gene expression of inflammatory cytokines in skeletal muscle, leading to further infiltration of phagocytes into damaged muscle tissue [4,7,8].…”

Section: Introductionmentioning

confidence: 99%

“…Phagocytes infiltrating muscle tissue play essential roles in tissue repair, such as protein degradation and removal of cellular debris, whereas activated phagocytes release pro-inflammatory cytokines and reactive oxygen species (ROS), and induce inflammation as well as myofiber membrane lysis [4,7]. In addition, ROS derived from phagocytes enhance gene expression of inflammatory cytokines in skeletal muscle, leading to further infiltration of phagocytes into damaged muscle tissue [4,7,8]. Thus, the production of inflammatory cytokines from the redox-sensitive pathway is also considered to be partly involved in muscle damage.…”

Section: Introductionmentioning

confidence: 99%

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Involvement of Neutrophil Dynamics and Function in Exercise-Induced Muscle Damage and Delayed-Onset Muscle Soreness: Effect of Hydrogen Bath

et al. 2018

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The purpose of this study was to investigate the involvement of neutrophil dynamics and function in exercise-induced muscle damage (EIMD) and delayed-onset muscle soreness (DOMS), and the effect of molecular hydrogen (H2) intake on these parameters. Nine healthy and active young men performed H2 and placebo bath trial in a crossover design. They carried out downhill running (−8% slope) for 30 min at a speed corresponding to 75~85% of peak oxygen uptake (VO2peak). Subsequently, they repeated bathing for 20 min per day for one week. Degree of muscle soreness (visual analogue scale: VAS), peripheral leukocyte counts, neutrophil dynamics and function, muscle damage, and inflammation markers were measured. Plasma interleukin (IL)-6 concentration was significantly correlated with peripheral neutrophil count, VAS, and serum creatine kinase activity, respectively, after downhill running. Peripheral neutrophil count and serum myoglobin concentration were also significantly correlated. Conversely, there were no effects of H2 bath. These results suggest that IL-6 may be involved in the mobilization of neutrophils into the peripheral blood and subsequent EIMD and DOMS after downhill running; however, it is not likely that H2 bath is effective for the inflammatory process that is centered on neutrophils after downhill running.

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The effect of macadamia oil intake on muscular inflammation and oxidative profile kinetics after exhaustive exercise

Malvestiti¹,

Borges²,

Weimann³

et al. 2017

Euro J Lipid Sci & Tech

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In this study, we evaluated the onset and resolution of inflammation in control and macadamia oil‐supplemented rats after a single session of exhaustive exercise. We also analysed the antioxidant enzymes catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), and thiobarbituric acid reactive substances (TBARS). Rats were supplemented for ten days with macadamia oil (25 mg/kg), which is rich in oleic and palmitoleic acids. On the 10th day, control and macadamia oil‐treated rats exercised to physical exhaustion. Plasma and muscular levels of pro‐inflammatory cytokines (tumour necrosis factor alpha [TNF‐α], interleukin‐1 beta [IL‐1β], IL‐6, cytokine‐induced neutrophil chemoattractant‐3 [CINC‐3], macrophage inflammatory protein‐3‐alpha [MIP‐3α]) and soluble L‐selectin were measured prior to, immediately after, and 2, 24, and 48 h after exercise. Our data revealed increases in the muscle concentrations of IL‐1β, L‐selectin, CINC‐3, and MIP‐3α in the group supplemented with macadamia oil compared with the concentrations in the control group. CAT also increased in the treated group, which is important because pro‐inflammatory cytokines precede growth factor production and might alter the muscle repair process. We concluded that inflammation resulting from exhaustive exercise was greater in animals that received macadamia oil than in control animals. Practical applications: There are potential benefits of using fatty acids in the treatment of injured muscles, and these substances have been used in several types of injured tissues. Macadamia oil intake alters muscle healing signaling by influencing the production of reactive oxygen species and inflammatory mediators. Therefore, it is reasonable to hypothesize that the intake of immunomodulatory supplements would be beneficial to the muscular recovery of an athlete following a strenuous exercise session. The muscle healing process has three phases: inflammation, new tissue formation and tissue remodeling, and these phases might be modulated by fatty acids. After an exhaustive exercise session, muscle inflammatory markers from macadamia oil‐supplemented rats are augmented in comparison to non‐supplemented animals. Our data revealed increases in the muscle concentrations of IL‐1β, L‐selectin, CINC‐3, and MIP‐3α in the group supplemented with macadamia oil compared with the concentrations in the control group.

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Neutrophil Depletion Attenuates Muscle Injury after Exhaustive Exercise

Abstract: These results suggest that neutrophils contribute to exacerbating muscle injury by regulating inflammation through the induction of macrophage infiltration.

Cited by 57 publications

References 35 publications

Exhaustive Exercise-Induced Neutrophil-Associated Tissue Damage and Possibility of its Prevention

Exhaustive Exercise-Induced Neutrophil-Associated Tissue Damage and Possibility of its Prevention

Involvement of Neutrophil Dynamics and Function in Exercise-Induced Muscle Damage and Delayed-Onset Muscle Soreness: Effect of Hydrogen Bath

The effect of macadamia oil intake on muscular inflammation and oxidative profile kinetics after exhaustive exercise

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