Involvement of Neutrophil Dynamics and Function in Exercise-Induced Muscle Damage and Delayed-Onset Muscle Soreness: Effect of Hydrogen Bath

Kawamura, Takanobu; Takahashi, Masaki; Tomari, Miki; Hara, Reira; Gando, Yuko; Muraoka, Isao

doi:10.3390/antiox7100127

Cited by 32 publications

(41 citation statements)

References 31 publications

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“…As described above, there is no conclusive evidence regarding the effectiveness of H 2 bathing against muscle damage, secondary oxidative stress, and inflammation after eccentric exercise [21,22]. Moreover, although the associated mechanism of action has not been elucidated, our results have shown that H 2 may alleviate DOMS after eccentric exercise [21].…”

Section: H 2 Bathingmentioning

confidence: 55%

“…Although the effectiveness of oral intake of H 2 water [18,19] and H 2 bathing [21,22] has not been demonstrated, intravenous infusion of H 2 -saline [23] and inhalation of H 2 gas [24] have been reported to suppress exercise-induced oxidative stress and/or inflammation. However, the antioxidant and anti-inflammatory effects of H 2 intake have been observed only in animals [23,24], and these effects have not been confirmed in humans regardless of the administration route [18,19,21,22]. Regarding the timing of H 2 administration, it seems to be most effective before [23] or simultaneously with exercise [24].…”

Section: Summary Of the Results Of Previous Studiesmentioning

confidence: 99%

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Application of Molecular Hydrogen as a Novel Antioxidant in Sports Science

Kawamura

Higashida

Muraoka

2020

Oxidative Medicine and Cellular Longevity

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Molecular hydrogen (H2) is a colorless, tasteless, odorless, and minimal molecule with high flammability. Although H2 has been thought to be an inert gas in living bodies for many years, an animal study reported that inhalation of H2 gas decreased oxidative stress and suppressed brain injury caused by ischemia and reperfusion injury due to its antioxidant action. Since then, the antioxidant action of H2 has attracted considerable attention and many studies have reported on its benefits. Most studies have reported the effects of H2 on diseases such as cancer, diabetes, cerebral infarction, and Alzheimer’s disease. However, little is known regarding its effects on healthy subjects and exercise. Thus far, including our study, only 6 studies have explored the effect of H2 on exercise. H2 is the smallest molecule and therefore can easily penetrate the cellular membrane and rapidly diffuse into organelles. H2 is thought to be able to selectively reduce hydroxyl radicals and peroxynitrite and does not affect physiologically reactive species. H2 can be supplied to the body through multiple routes of administration, such as oral intake of H2 water and H2 bathing. Therefore, H2 may be a potential alternative strategy for conventional exogenous antioxidant interventions in sports science. The purpose of this review is to provide evidence regarding the effects of H2 intake on changes in physiological and biochemical parameters, centering on exercise-induced oxidative stress, for each intake method. Furthermore, this review highlights possible future directions in this area of research.

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Section: H 2 Bathingmentioning

confidence: 55%

Section: Summary Of the Results Of Previous Studiesmentioning

confidence: 99%

Application of Molecular Hydrogen as a Novel Antioxidant in Sports Science

Kawamura

Higashida

Muraoka

2020

Oxidative Medicine and Cellular Longevity

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“…Prolonged strenuous exercise may induce unfavorable biological changes and symptoms, including inflammatory responses, such as leukocyte infiltration [1][2][3]; gastrointestinal (GI) incidents, such as diarrhea, nausea, and gastric pain [4][5][6]; delayed-onset muscle soreness; muscle and internal organ injury; and immune suppression [7][8][9][10][11].…”

Section: Introductionmentioning

confidence: 99%

Effects of an 8-Week Protein Supplementation Regimen with Hyperimmunized Cow Milk on Exercise-Induced Organ Damage and Inflammation in Male Runners: A Randomized, Placebo Controlled, Cross-Over Study

Tominaga

Kanda

et al. 2020

Biomedicines

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Prolonged strenuous exercise may induce inflammation, cause changes in gastrointestinal permeability, and lead to other unfavorable biological changes and diseases. Nutritional approaches have been used to prevent exercise-induced inflammatory responses and gastrointestinal disorders. Hyperimmunized milk, obtained by immunizing cows against specific antigens, promotes the development of immunity against pathogens, promotes anti-inflammatory effects, and protects intestinal function. Immune protein (IMP) is a concentrated product of hyperimmunized milk and is a more promising means of supplementation to protect against acute infections and inflammation.To determine whether IMP has protective properties against exercise-induced gastrointestinal dysfunction and inflammation, we examined biochemical markers, intestinal damage markers, and pro-/anti-inflammatory profiles of young male runners using a randomized, placebo controlled, cross-over design. Urine samples were collected and used for measurements of creatinine, N-acetyl-β-d-glucosaminidase, osmotic pressure, and specific gravity. Titin was measured as a muscle damage marker. Further, urine concentrations of complement 5a, calprotectin, fractalkine, myeloperoxidase, macrophage colony-stimulating factor, monocyte chemotactic protein-1, intestinal fatty acid binding protein (I-FABP), interferon (IFN)-γ, interleukin (IL)-1β, IL-1 receptor antagonist, IL-2, IL-4, IL-6, IL-8, IL-10, IL-12p40, and tumor necrosis factor (TNF)-α were measured by enzyme-linked immunosorbent assays. We demonstrated that urine osmotic pressure, urine specific gravity, I-FABP, IFN-γ, IL-1β, and TNF-α were reduced by 8 weeks of IMP supplementation, indicating that IMP may have potential in preventing strenuous exercise-induced renal dysfunction, increased intestinal permeability, and inflammation. Thus, IMP supplementation may be a feasible nutritional approach for the prevention of unfavorable exercise-induced symptoms.

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“…Chronic exhaustive exercise might cause mechanical injury of muscles and activates macrophages, which results in increased synthesis and secretion of proinflammatory cytokines, such as interleukin-1β (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α). These cytokines also stimulate macrophages to secrete prostaglandins causing muscle soreness, and lead to exercise-induced fatigue (Pawlik et al, 2016;Kawamura et al, 2018;Yang et al, 2019). In addition, the hippocampus is an important brain region that is vulnerable to stress and is involved in the exercise-induced fatigue (Zhu et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Effects of Mongolian Warm Acupuncture on iNOS/NO and Inflammatory Cytokines in the Hippocampus of Chronic Fatigue Rats

Shui

et al. 2020

Front. Integr. Neurosci.

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The inducible nitric oxide synthase/nitric oxide (iNOS/NO) signaling pathway and inflammatory cytokines play important roles in the pathogenesis of exercise-induced fatigue. Studies have found that Mongolian warm acupuncture (WA) could alleviate exercise-induced fatigue. However, the exact mechanisms underlying its effects remain unclear. In the present study, we investigated the effects of Mongolian WA on iNOS/NO signaling pathway and proinflammatory cytokines in a chronic exhaustive swimminginduced fatigue rat model. Animals were randomly divided into Control group, Ctrl + WA group, Model group, and Model + WA group. The body weight, exhaustive swimming time test, and Morris water maze test were performed before and after the chronic exhaustive swimming. The serum levels of interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), and iNOS were detected by enzyme linked immunosorbent assay (ELISA). The mRNA expressions of IL-1β, IL-6, TNF-α, IFN-γ, and iNOS in the hippocampus were measured by real-time polymerase chain reaction (RT-PCR). Moreover, the protein expression of iNOS in the hippocampus was measured by western blot, and the NO productions in the serum and hippocampus were detected by Griess reaction system. Chronic exhaustive exercise significantly reduced the body weight and exhaustive swimming time, and induced impairment in learning and memory, and which were reversed by WA treatment. Chronic exhaustive exercise also increased the expressions of iNOS and proinflammatory cytokines, while WA treatment significantly decreased the level of iNOS and proinflammatory cytokines. However, chronic exhaustive exercise did not affect the NO production. These findings demonstrated that WA could alleviate the chronic exhaustive swimming-induced fatigue and improve the learning and memory ability, and the actions might be related to the reduction of inflammatory response and iNOS expression.

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Involvement of Neutrophil Dynamics and Function in Exercise-Induced Muscle Damage and Delayed-Onset Muscle Soreness: Effect of Hydrogen Bath

Cited by 32 publications

References 31 publications

Application of Molecular Hydrogen as a Novel Antioxidant in Sports Science

Application of Molecular Hydrogen as a Novel Antioxidant in Sports Science

Effects of an 8-Week Protein Supplementation Regimen with Hyperimmunized Cow Milk on Exercise-Induced Organ Damage and Inflammation in Male Runners: A Randomized, Placebo Controlled, Cross-Over Study

Effects of Mongolian Warm Acupuncture on iNOS/NO and Inflammatory Cytokines in the Hippocampus of Chronic Fatigue Rats

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