Neutron scattering: a tool to detect
            <i>in vivo</i>
            thermal stress effects at the molecular dynamics level in micro-organisms

Marty, Vincent; Jasnin, Marion; Fabiani, E.; Vauclare, Pierre; Gabel, Frank; Trapp, Marcus; Peters, Judith; Zaccaı̈, Giuseppe; Franzetti, Bruno

doi:10.1098/rsif.2013.0003

Cited by 24 publications

(20 citation statements)

References 23 publications

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“…They are composed of several compartments (membrane, cytoplasm, skeleton, ribosomes), which are composed of lipids, cholesterol, proteins, nucleic acids, water, and numerous other molecules. Thus reproducibility of the experiments may be an issue, even when cells are prepared with the greatest care [23,24]. Neutron scattering experiments are long (typically several days) and usually require the isolation of the cells from nutriments or the use of physico-chemical conditions far from the cell optima.…”

Section: Summed Neutron Intensitiesmentioning

confidence: 99%

Deep Sea Microbes Probed by Incoherent Neutron Scattering Under High Hydrostatic Pressure

Peters¹,

Martínez²,

Michoud

et al. 2014

Zeitschrift Für Physikalische Chemie

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The majority of the biosphere is a high pressure environment. Around 70% of the marine biosphere lies at depths below 1000 m, i.e. at pressures of 100 bars or higher. To survive in these environments, deep-biosphere organisms have adapted to life at high pressure. In vitro studies showed that the activity of certain proteins originating from deep-sea organisms is less affected by high pressure than that of enzymes from surface organisms [1-3]. However, the genetic and structural bases for this increased pressure resistance are still unknown. Elastic incoherent neutron scattering studies, which provide access to information about molecular dynamics, constitute a very promising approach to decipher the structural adaptation in proteins living under high pressure. This approach has been used in the past to investigate the adaptation of biological systems to temperature and salinity and proved to be essential and complementary to structural studies. Here first investigations of high pressure effects on cell dynamics are presented using Thermococcales as models.

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Section: Summed Neutron Intensitiesmentioning

confidence: 99%

Deep Sea Microbes Probed by Incoherent Neutron Scattering Under High Hydrostatic Pressure

Peters¹,

Martínez²,

Michoud

et al. 2014

Zeitschrift Für Physikalische Chemie

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“…marismortui and of the native proteome from Hbt. salinarum [12][13][14][15][16] indicated that halophilic proteins display specific biophysical adaptation properties to high salt reflected by higher structural resilience, compared to mesophilic systems. It is likely, therefore, that most halophilic proteins display an obligatory requirement for high salt conditions in order to be active and stable.…”

mentioning

confidence: 99%

Surviving salt fluctuations: stress and recovery in Halobacterium salinarum, an extreme halophilic Archaeon

Vauclare

Natali

Kleman

et al. 2020

Sci Rep

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“…To use a mechanical analogy: in a collision between a moving billiard ball and one that is anchored by a spring, the force constant of the spring as well as the vibration amplitude of the anchored ball can be calculated from the changes in energy and momentum of the moving ball after it is ‘scattered’. Systems studied by neutron scattering include intrinsically disordered proteins, whole cells, membranes, nanoparticles and tissue222324252627.…”

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confidence: 99%

The fluctuating ribosome: thermal molecular dynamics characterized by neutron scattering

Zaccaı̈

Natali

Peters

et al. 2016

Sci Rep

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Conformational changes associated with ribosome function have been identified by X-ray crystallography and cryo-electron microscopy. These methods, however, inform poorly on timescales. Neutron scattering is well adapted for direct measurements of thermal molecular dynamics, the ‘lubricant’ for the conformational fluctuations required for biological activity. The method was applied to compare water dynamics and conformational fluctuations in the 30 S and 50 S ribosomal subunits from Haloarcula marismortui, under high salt, stable conditions. Similar free and hydration water diffusion parameters are found for both subunits. With respect to the 50 S subunit, the 30 S is characterized by a softer force constant and larger mean square displacements (MSD), which would facilitate conformational adjustments required for messenger and transfer RNA binding. It has been shown previously that systems from mesophiles and extremophiles are adapted to have similar MSD under their respective physiological conditions. This suggests that the results presented are not specific to halophiles in high salt but a general property of ribosome dynamics under corresponding, active conditions. The current study opens new perspectives for neutron scattering characterization of component functional molecular dynamics within the ribosome.

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Neutron scattering: a tool to detect in vivo thermal stress effects at the molecular dynamics level in micro-organisms

Cited by 24 publications

References 23 publications

Deep Sea Microbes Probed by Incoherent Neutron Scattering Under High Hydrostatic Pressure

Deep Sea Microbes Probed by Incoherent Neutron Scattering Under High Hydrostatic Pressure

Surviving salt fluctuations: stress and recovery in Halobacterium salinarum, an extreme halophilic Archaeon

The fluctuating ribosome: thermal molecular dynamics characterized by neutron scattering

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