Surviving salt fluctuations: stress and recovery in Halobacterium salinarum, an extreme halophilic Archaeon

Vauclare, Pierre; Natali, Francesca; Kleman, Jean-Philippe; Zaccaı̈, Giuseppe; Franzetti, Bruno

doi:10.1038/s41598-020-59681-1

Cited by 28 publications

(22 citation statements)

References 40 publications

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“…Cell morphology of Hbt. salinarum changes from rod-shaped to circular in the presence of low salt (49,50). Our previous work demonstrated that the ∆hpyA strain exhibits similar circularity in standard conditions (21).…”

Section: Hpya Is Important For Wild Type Growth and Morphology In Low Salinity Stress Conditionsmentioning

confidence: 95%

An archaeal histone-like protein regulates gene expression in response to salt stress

Sakrikar

Schmid

2021

Preprint

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Histones, ubiquitous in eukaryotes as DNA-packing proteins, find their evolutionary origins in archaea. Unlike the characterized histone proteins of a number of methanogenic and themophilic archaea, previous research indicated that HpyA, the sole histone encoded in the model halophile Halobacterium salinarum, is not involved in DNA packaging. Instead, it was found to have widespread but subtle effects on gene expression and to maintain wild type cell morphology; however, its precise function remains unclear. Here we use quantitative phenotyping, genetics, and functional genomic to investigate HpyA function. These experiments revealed that HpyA is important for growth and rod-shaped morphology in reduced salinity. HpyA preferentially binds DNA at discrete genomic sites under low salt to regulate expression of ion uptake, particularly iron. HpyA also globally but indirectly activates other ion uptake and nucleotide biosynthesis pathways in a salt-dependent manner. Taken together, these results demonstrate an alternative function for an archaeal histone-like protein as a transcriptional regulator, with its function tuned to the physiological stressors of the hypersaline environment.

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Section: Hpya Is Important For Wild Type Growth and Morphology In Low Salinity Stress Conditionsmentioning

confidence: 95%

An archaeal histone-like protein regulates gene expression in response to salt stress

Sakrikar

Schmid

2021

Preprint

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“…Therefore, suitable haloarchaeon expression systems are often necessary. Successful homologous expression of haloarchaeal proteins has been reported in halophilic archaeal hosts, such as Halobacterium salinarum ( Nomura and Harada, 1998 ; Kixmüller and Greie, 2012 ; Vauclare et al, 2020 ) and Haloferax volcanii ( Hfx. volcanii ) ( Pohlschroder and Schulze, 2019 ; Haque et al, 2020 ).…”

Section: Mining Of the Red Sea Enzyme Poolmentioning

confidence: 99%

Novel Enzymes From the Red Sea Brine Pools: Current State and Potential

et al. 2021

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The Red Sea is a marine environment with unique chemical characteristics and physical topographies. Among the various habitats offered by the Red Sea, the deep-sea brine pools are the most extreme in terms of salinity, temperature and metal contents. Nonetheless, the brine pools host rich polyextremophilic bacterial and archaeal communities. These microbial communities are promising sources for various classes of enzymes adapted to harsh environments – extremozymes. Extremozymes are emerging as novel biocatalysts for biotechnological applications due to their ability to perform catalytic reactions under harsh biophysical conditions, such as those used in many industrial processes. In this review, we provide an overview of the extremozymes from different Red Sea brine pools and discuss the overall biotechnological potential of the Red Sea proteome.

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“…On the contrary, non-halophiles do not grow in the environment containing above 0.2M (1%) NaCl, and halotolerant organisms are viable in the presence or absence of highly saline conditions, but it is not necessary for their optimal growth [5]. Moreover, halophilic and halotolerant organisms are able to adapt to a broad range of salt concentrations, occurring seasonally, annually, or irregularly in their natural environments [4,6].…”

Section: Introductionmentioning

confidence: 99%

The Methods of Digging for “Gold” within the Salt: Characterization of Halophilic Prokaryotes and Identification of Their Valuable Biological Products Using Sequencing and Genome Mining Tools

Lach

Jecz

Strapagiel

et al. 2021

Genes

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Halophiles, the salt-loving organisms, have been investigated for at least a hundred years. They are found in all three domains of life, namely Archaea, Bacteria, and Eukarya, and occur in saline and hypersaline environments worldwide. They are already a valuable source of various biomolecules for biotechnological, pharmaceutical, cosmetological and industrial applications. In the present era of multidrug-resistant bacteria, cancer expansion, and extreme environmental pollution, the demand for new, effective compounds is higher and more urgent than ever before. Thus, the unique metabolism of halophilic microorganisms, their low nutritional requirements and their ability to adapt to harsh conditions (high salinity, high pressure and UV radiation, low oxygen concentration, hydrophobic conditions, extreme temperatures and pH, toxic compounds and heavy metals) make them promising candidates as a fruitful source of bioactive compounds. The main aim of this review is to highlight the nucleic acid sequencing experimental strategies used in halophile studies in concert with the presentation of recent examples of bioproducts and functions discovered in silico in the halophile’s genomes. We point out methodological gaps and solutions based on in silico methods that are helpful in the identification of valuable bioproducts synthesized by halophiles. We also show the potential of an increasing number of publicly available genomic and metagenomic data for halophilic organisms that can be analysed to identify such new bioproducts and their producers.

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Surviving salt fluctuations: stress and recovery in Halobacterium salinarum, an extreme halophilic Archaeon

Abstract: Flow cytometry analysis of Hbt. salinarum cells structural integrity under low salt stress and high salt reactivation. The viability of stressed Hbt. salinarum cells was assessed by flow cytometry analysis 33 .

Cited by 28 publications

References 40 publications

An archaeal histone-like protein regulates gene expression in response to salt stress

An archaeal histone-like protein regulates gene expression in response to salt stress

Novel Enzymes From the Red Sea Brine Pools: Current State and Potential

The Methods of Digging for “Gold” within the Salt: Characterization of Halophilic Prokaryotes and Identification of Their Valuable Biological Products Using Sequencing and Genome Mining Tools

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