Neutron activation analysis and X-ray Rayleigh and Raman scattering of hair and nail clippings as noninvasive bioindicators for Cu liver status in Labrador Retrievers

Bode, P.; Bueno, Maria Izabel M. S.; Bortoleto, Gisele Gonçalves; Hoffmann, Gaby; Ingh, T.S.G.A.M. van den; Rothuizen, J.

doi:10.1007/s00216-008-1873-7

Cited by 15 publications

(10 citation statements)

References 14 publications

(14 reference statements)

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“…Grading and staging of biopsy samples was performed according to the guidelines described by the WSAVA . A third biopsy specimen was collected in a copper free container and freeze‐dried before quantitative copper determination by Instrumental Neutron Activation Analysis . Quantitative copper (CuQ) concentrations >400 mg/kg dry weight liver (dwl) were considered to be abnormally elevated…”

Section: Methodsmentioning

confidence: 99%

Sensitivity and Specificity of PlasmaALT,ALP, and Bile Acids for Hepatitis in Labrador Retrievers

Dirksen

Burgener

Rothuizen

et al. 2017

Veterinary Internal Medicne

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BackgroundBiochemical indicators for diagnosing liver disease are plasma alanine aminotransferase activity (ALT), alkaline phosphatase activity (ALP), and bile acid concentration (BA).ObjectivesTo determine the sensitivity and specificity of ALT, ALP, and BA for detecting primary hepatitis (PH) in clinically healthy Labrador retrievers and investigate whether ALT and ALP can discriminate between dogs with PH and nonspecific reactive hepatitis (RH).Animals191 clinically healthy and 51 clinically ill Labrador retrievers with hepatic histopathology.MethodsRetrospective study. Medical records were reviewed for ALT, ALP, preprandial BA, liver histopathology, and hepatic copper concentrations.ResultsIn 64% (122/191) of the clinically healthy Labrador retrievers, hepatic histology revealed inflammatory infiltrates. This frequency might be biased because part of them was included as first‐line relatives of dogs with copper‐associated hepatitis. Sensitivity of ALT, ALP, and BA in this population for detecting acute hepatitis was 45, 15, and 15%, respectively. For chronic hepatitis, sensitivity was 71, 35, and 13%, respectively. Specificity of ALT, ALP, and BA was >90% for AH, CH, and RH. When increased liver enzymes were present, median ALT was significantly higher in PH cases (312 U/L, range 38–1,369) compared to RH cases (91 U/L, range 39–139) (P < .001). There was no difference in ALP between dogs with a PH and a RH (P = .361).Conclusions and Clinical ImportanceHistopathologic abnormalities in the liver were present in the majority of apparent clinically healthy Labrador retrievers. The sensitivity of ALT, ALP, and BA for detecting acute and chronic hepatitis in this population was low. More sensitive biomarkers are needed for early detection of liver disease in apparent clinically healthy dogs.

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Section: Methodsmentioning

confidence: 99%

Sensitivity and Specificity of PlasmaALT,ALP, and Bile Acids for Hepatitis in Labrador Retrievers

Dirksen

Burgener

Rothuizen

et al. 2017

Veterinary Internal Medicne

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“…Результаты, полученные в ходе исследований по применению РФА для ногтей представлены авторами работ [66,253,[265][266][267][268][269][270][271][272][273][274][275][276]…”

Section: применение рфа для исследования волос и ногтейunclassified

X-Ray Fluorescence Analysis in Biology and Medicine

Ревенко¹

2020

АиК

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X-ray fluorescence analysis (XRF) is widely used when determining the concentrations of elements in various materials in biological research. Current review considers the achievements related to the features of the XRF method. X-ray fluorescence analysis in most cases is a non-destructive method that has proven its potential for measuring the concentrations of elements with a high atomic number Z in organs and tissues in vivo. The main areas of its application in biology and medicine are content determination of basic and toxic elements in plants, samples of bones, teeth, hairs, nails and tissues of vital organs, body fluids such as blood, serum, plasma, saliva, urine, etc. Usually, these studies are part of applied programs that include environmental and metabolic analyses of the population, including the influence of professional factors. The advantages of XRF are also discussed with regards to biological materials. It is noted that the form of the element in the sample practically does not affect the analysis result. In recent years, several new XRF spectrometers models have been designed, where polycapillary lenses and half lenses are used as collimating systems. This is important in the case of in vivo application of X-ray fluorescence determination of certain elements in bones and tissues. The dynamic development is typical for detectors with thermoelectric cooling. Variants of preparation of biological materials for analysis are discussed (grinding, dry or wet ashing, acid decomposition, use of suspensions in the case of XRF with TIR). The results of evaluating the interelement effects for XRF of materials of plant origin, including herbs, spices, apple leaves, birch and tomato leaves, tea, wheat, rye, rice, oatmeal, flax flour, beans, ground, and instant coffee are presented. Examples of the use of XRF in various types of forensic research are considered: poisoning, counterfeiting of drugs and food brands, dental implants, identification of remains. The review presents examples of the participation of Russian X-ray physicists in solving the problems under consideration. The list of references is 400 papers, mainly including the publications of the last 20 years.

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“…14 Assim, a XRF pode ser aplicada na resolução de vários problemas analíticos, seja em medicina, 15 geologia, 16 biologia, 17 arqueologia 18 ou monitoramento ambiental.…”

Section: Elementounclassified

Análise exploratória rápida e não destrutiva (screening) da presença de elementos químicos tóxicos em material escolar por fluorescência de raios x

Da-Col¹,

Sanchez²,

Terra³

et al. 2013

Quím. Nova

Self Cite

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Recebido em 5/7/12; aceito em 30/11/12; publicado na web em 8/3/13 SCREENING INVESTIGATION OF TOXIC CHEMICAL ELEMENTS IN SCHOOL SUPPLIES BY X-RAY FLUORESCENCE. Compulsory in Brazil, toy certification aims to prevent possible risks in toy use. The Brazilian National Institute of Metrology (INMETRO) establishes the maximum concentration of toxic elements (TE) that may be present in toys. This study evaluates the presence of TE in different crayons, gouache and modeling clays using X-ray fluorescence. This technique is fast, has low operating cost and minimum sample pretreatment, resulting in a clean procedure without reagent consumption and waste generation. Bromine (in gouache) and Barium (in crayons) were the only potentially TE identified in the samples studied.Keywords: school supplies; screening; X-ray fluorescence. INTRODUÇÃOProdutos como massa de modelar, tinta guache e giz de cera não são apenas empregados como material escolar, mas também são utilizados em atividades por crianças que ainda não atingiram a idade escolar. Nesta fase da vida, devem ser redobrados os cuidados com possíveis contaminações por elementos químicos tóxicos, pois podem ocorrer problemas sérios e irreversíveis.

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Neutron activation analysis and X-ray Rayleigh and Raman scattering of hair and nail clippings as noninvasive bioindicators for Cu liver status in Labrador Retrievers

Cited by 15 publications

References 14 publications

Sensitivity and Specificity of PlasmaALT,ALP, and Bile Acids for Hepatitis in Labrador Retrievers

Sensitivity and Specificity of PlasmaALT,ALP, and Bile Acids for Hepatitis in Labrador Retrievers

X-Ray Fluorescence Analysis in Biology and Medicine

Análise exploratória rápida e não destrutiva (screening) da presença de elementos químicos tóxicos em material escolar por fluorescência de raios x

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