Neutralizing EBV‐specific IgA in throat washings of nasopharyngeal carcinoma (NPC) patients

Des̀granges, Claude; de-Thé, G; Ho, J. H. C.; Ellouz, R.

doi:10.1002/ijc.2910190506

Cited by 38 publications

(14 citation statements)

References 27 publications

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“…Ho et al (1978) postulated, therefore, that such a difference in the state of stimulation with the EBV antigens might also account for the almost exclusive detection of serum IgA anti-VCA in NPC patients. In support of the above contention, IgA antibodies to EBV have been detected in the saliva and throat washings obtained from a majority of NPC patients, but not from control subjects Desgranges et al, 1977). To study the local production of antibodies further, we have carried out an immunohistochemical investigation on sections of biopsy specimens obtained from NPC patients, and we report here an accumulation of IgA and IgG plasma cells in the connective tissues adjacent to nests of NPC cells.…”

mentioning

confidence: 51%

Immunohistochemistry of local immunoglobulin production in nasopharyngeal carcinoma

Ho¹,

Kwan²,

Ng³

1978

Br J Cancer

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Immunohistochemical investigations by the immunoperoxidase method have been carried out on sections of biopsy specimens obtained from the primary tumour sites of patients with nasopharyngeal carcinoma (NPC). It was found that, in many of the sections thus examined, there was an accumulation of plasma cells, particularly of the IgA type, in the connective tissues surrounding nests of NPC cells. Similar accumulation of plasma cells in the subepithelial connective tissues was likewise observed in a case of choanal polyp. Plasma cells were rarely observed in the section of a biopsy specimen of non-neoplastic oropharyngeal mucosa. These results indicated that the nasopharynx may be a site for the local production of IgA, but the antigenic specificity of these molecules is, however, not known. The possibility that the nasopharynx is a site for the local production of antibodies to the Epstein-Barr virus (EBV)-related antigens was discussed. Images Fig. 1 Fig. 2 Fig. 3 Fig. 4 Fig. 5 Fig. 6

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confidence: 51%

Immunohistochemistry of local immunoglobulin production in nasopharyngeal carcinoma

Ho¹,

Kwan²,

Ng³

1978

Br J Cancer

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“…There is no a priori reason to expect such a relationship. It is of interest, in this regard, that Desgranges et a1 (23) found that neutralizing antibody present in the saliva of several patients with nasopharyngeal carcinoma could block transforming activity, despite the fact that viral particles were demonstrable by electron microscopy in saliva concentrates. Our own preliminary investigation of EBV antibody activity in throat washings has revealed a low percentage of positivity that does not correlate with the presence of infective virus.…”

Section: Discussionmentioning

confidence: 99%

Oropharyngeal epstein‐barr virus excretion in rheumatoid arthritis

Depper¹,

Zvaifler²,

Bluestein³

1982

Arthritis & Rheumatism

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We hypothesized that the defective cellular regulation of Epstein-Barr virus (EBV) in rheumatoid arthritis (RA) might be reflected in an increased rate of oropharyngeal virus excretion, but we found that the prevalence of excretion in 45 RA patients (22%) did not differ from 45 age-and sex-matched non-RA patients (24 %) who were taking similar medications. Increased excretion rates correlated with corticosteroid therapy and male sex, but not with age or serum levels of EBV antibodies.The perpetuation of Epstein-Barr virus (EBV) is guaranteed by the regular appearance of infectious viral particles in the oropharynx of previously infected individuals. As many as 90% of patients with acute infectious mononucleosis shed virus for as long as 16 months after their illness (1-5). In addition, at any one time approximately 20% of healthy EBV seropositive individuals excrete virus (4,6); this figure rises to 50% in patients who take immunosuppressive therapy (6-8). The probable site of viral replication is within the salivary glands (5,9) and is presumed to be secondary to the reactivation of latently infected cells.In a number of recent observations (10-19),

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“…It should be noted that patients with NPC and Burkitt's lymphoma, two diseases that have been strongly linked to EBV, have not been found to have an increased frequency of oropharyngeal EBV shedding as detected in a transformation assay. Desgranges et al [28] found 6 of 26 (23%) throat washings from NPC patients were positive in a transforma tion assay compared to 22-30% of an appro priate control group from the same geographi cal area. Neutralizing antibodies found in the saliva of NPC patients may have interfered with detection of EBV in this type of assay.…”

Section: Discussionmentioning

confidence: 99%

“…Our subsequent data showed that this was not due to a decreased frequency of detectable EBV in the orophar ynx of RA patients since EBV was found in throat washings. Desgranges and colleagues [28] have reported that neutralizing antibody in saliva of patients with nasopharyngeal car cinoma (NPC) can prevent detection of EBV by transformation assays even though viral particles can be seen in saliva by electron microscopy. It is possible that neutralizing antibody in RA parotid secretions could have prevented EBV detection.…”

Section: Discussionmentioning

confidence: 99%

Frequency of Transforming Epstein-Barr Virus in Oropharyngeal Secretions of Rheumatoid Arthritis Patients

Aitcheson¹,

Wilson²,

Perrell³

et al. 1983

Intervirology

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To determine if rheumatoid arthritis (RA) patients have an increased frequency of Epstein-Barr virus (EBV) shedding into saliva, throat washings were collected from 59 patients with RA and 64 healthy adult controls. EBV in filtered samples was detected by transformation of human umbilical cord lymphocytes. EBV was detected in 27% (16/59) of throat washings of RA patients compared to 11% (7/64) of control samples. This difference is significant (p < 0.05). RA patients on steroids had a frequency of positivity of 43% (10/23) compared to 17% (6/36) in patients not on steroids. No correlation was demonstrated between steroid dose received by the patient and detection of EBV in throat washings. Parotid fluid samples were collected from 18 RA patients and 11 healthy controls and were tested for EBV by the transformation bioassay. EBV was detected in 18% (2/11) of samples from normal controls, but samples from RA patients were uniformly negative.

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Neutralizing EBV‐specific IgA in throat washings of nasopharyngeal carcinoma (NPC) patients

Cited by 38 publications

References 27 publications

Immunohistochemistry of local immunoglobulin production in nasopharyngeal carcinoma

Immunohistochemistry of local immunoglobulin production in nasopharyngeal carcinoma

Oropharyngeal epstein‐barr virus excretion in rheumatoid arthritis

Frequency of Transforming Epstein-Barr Virus in Oropharyngeal Secretions of Rheumatoid Arthritis Patients

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