Neutralizing capacity of antisera raised in horses and rabbits against Crotalus durissus terrificus (South American rattlesnake) venom and its main toxin, crotoxin

Oshima-Franco, Yoko; Hyslop, Stephen; Prado-Franceschi, J.; Cruz‐Höfling, Maria Alice da; Rodrigues‐Simioni, Léa

doi:10.1016/s0041-0101(98)00246-3

Cited by 39 publications

(21 citation statements)

References 32 publications

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“…The Brazilian Crotalus durissus terrificus snake is responsible for many clinical cases of envenoming. Its venom contains a variety of toxic proteins including crotoxin, crotamine, gyroxin, convulsin, and a thrombin-like enzymes [2, 3]. Envenomation caused by Crotalus snake leads to serious complications and alterations such as systemic hemorrhage, hepatotoxicity, myotoxicity, hypotension, acute renal failure, and shock [4–7].…”

Section: Introductionmentioning

confidence: 99%

Inflammatory Mediators Release in Urine from Mice Injected withCrotalus durissus terrificusVenom

Cruz

Navarro

Mendonça

et al. 2011

Mediators of Inflammation

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In this study, we investigated in groups of female BALB/c mice injected with Crotalus durissus terrificus venom (Cdt) the renal function based on creatinine clearance, percentage of fractional excretion cytokines and histological examination of renal tissue. Cdt caused renal alterations that induced proteinuria during the initial hours post-venom and reduced creatinine clearance 15 min. up to 2 hours post-venom administration. In urine from mice injected with Cdt induced a decrease in IL-4 levels. More pronounced increments of IL-5, IL-6 and IFN-γ were observed after 15 and 30 min, respectively. The highest levels of TNF and IL-10 were observed at 1 and 4 hs, respectively. The ratios of pro- and anti-inflammatory cytokines in animals injected with Cdt, which may be manifested in the inflammatory status during the envenoming. In groups of animals treated with Cdt were observed a decreasing in creatinine clearance and its effect on glomerular filtration rate was accompanied by decreased fractional excretion of cytokines and morphologic disturbances. This loss of change selectively in envenomation could thus explain why the relatively excretion of cytokines is reduced while of total proteins increases. In conclusion the fractional excretion of cytokines is significantly reduced in mice injected with Cdt, despite proteinuria.

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Section: Introductionmentioning

confidence: 99%

Inflammatory Mediators Release in Urine from Mice Injected withCrotalus durissus terrificusVenom

Cruz

Navarro

Mendonça

et al. 2011

Mediators of Inflammation

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show abstract

“…Our experience with Dipteryx alata Vogel, 19 one of many betulin-plants, 2 showed an unexplained facilitatory effect on mouse nerve-muscle synapse, 9 besides it possesses antiophidian properties against the neuromuscular and myotoxicity, two known toxic effects of Bothrops jararacussu, 20 and Crotalus durissus terrificus 21 22 Thus, the evaluation of betulin mutagenicity is an inevitable step in the safety assessment. Here, the Ames Salmonella/mutagenicity assay, a shortterm bacterial assay, was chosen for testing betulin, since it is able in identifying substances that can produce genetic damage that leads to gene mutations.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Betulin Mutagenicity by Salmonella/Microsome Test

et al. 2016

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“…However, adverse reactions mainly associated with the Fc activating complement ability of horse IgG have been described (21). For cases of hypersensitivity to horse serum, other animals have been tested as source of antivenoms; good results have been observed with goats, rabbits, and sheep (23,24,29).…”

Section: Discussionmentioning

confidence: 99%

Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming

Ferreira¹,

Bastos²,

Pelizon³

et al. 2005

J. Venom. Anim. Toxins incl. Trop. Dis

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Alternative sources of anti-ophidic serum are being investigated due to the secondary effects associated with types I and II hypersensitivity reactions. In the present study we raised and evaluated the protective effect of an ovine antibothropic serum in a Swiss mice envenoming model. Ovine antiserum was obtained by immunization with seven increasing doses of bothropic venom associated with adjuvants. The neutralizing ability was tested by the lethal activity (2 LD 50 ) neutralization and serum and splenic venom levels after antivenom administration to experimentally envenomed mice. The antiserum effect on local edema was also tested by injection of venom/antivenom mixtures into the mice footpads. Ovine antiserum neutralized lethal activity and also significantly decreased serum and splenic venom levels. However, this antiserum was not able to mediate any protective effect on edema triggered by bothropic venom.

show abstract

Neutralizing capacity of antisera raised in horses and rabbits against Crotalus durissus terrificus (South American rattlesnake) venom and its main toxin, crotoxin

Cited by 39 publications

References 32 publications

Inflammatory Mediators Release in Urine from Mice Injected withCrotalus durissus terrificusVenom

Inflammatory Mediators Release in Urine from Mice Injected withCrotalus durissus terrificusVenom

Evaluation of Betulin Mutagenicity by Salmonella/Microsome Test

Assessment of the neutralizing potency of ovine antivenom in a swiss mice model of Bothrops jararaca envenoming

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