Neutralizing antibody but not hemagglutination antibody provides accurate evaluation for protective immune response to H5N1 avian influenza virus in vaccinated rabbits

Zhu, Han-Ping; Ding, Xiaohang; Chen, Xuekui; Yao, Pingping; Fang, Xu; Xie, Rong-hui; Yang, Zhanynv; Liang, Weifeng; Zhang, Yiqing; Yan-Jin, LI; Shen, Jiayu; He, P.; Guo, Zhijun; Su, Bo; Sun, Shubin; Zhang, Zhu

doi:10.1016/j.vaccine.2011.05.067

Cited by 10 publications

(4 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…One of the immunogenicity criteria for licensure of new vaccines is that at least 70% of vaccine recipients achieve postvaccination HAI titers ≥1:40 [5,6]. Moreover, there are few studies exploring the correlation of other measures of humoral immunity, such as from viral-neutralization assays, with protection against influenza virus infection in community settings [7][8][9].…”

mentioning

confidence: 99%

Association Between Antibody Titers and Protection Against Influenza Virus Infection Within Households

Tsang

Cauchemez

Perera³

et al. 2014

The Journal of Infectious Diseases

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 99%

Association Between Antibody Titers and Protection Against Influenza Virus Infection Within Households

Tsang

Cauchemez

Perera³

et al. 2014

The Journal of Infectious Diseases

View full text Add to dashboard Cite

show abstract

“…The antibodies were tested by the hemagglutination inhibition (HI) test. [14][15][16] To obtain the titer of the virus required for immunization, the experiment was performed with different titers, from titers 8, 16, 32, 64 to titer 128. It was determined that titer 32 was the lowest titer which produced good immunity.…”

Section: Preparation Of Antibodiesmentioning

confidence: 99%

Antigenic Variation in H5N1 clade 2.1 Viruses in Indonesia from 2005 to 2011

et al. 2013

View full text Add to dashboard Cite

Influenza A (H5N1) virus, has spread to several countries in the world and has a high mortality rate. Meanwhile, the virus has evolved into several clades. The human influenza A (H5N1) virus circulating in Indonesia is a member of clade 2.1, which is different in antigenicity from other clades of influenza A (H5N1). An analysis of the antigenic variation in the H5 hemagglutinin gene (HA) of the influenza A (H5N1) virus strains circulating in Indonesia has been undertaken. Several position of amino acid mutations, including mutations at positions 35, 53, 141, 145, 163, 174, 183, 184, 189, and 231, have been identified. The mutation Val-174-Iso appears to play an important role in immunogenicity and cross-reactivity with rabbit antisera. This study shows that the evolution of the H5HA antigenic variation of the influenza A (H5N1) virus circulating in Indonesia from 2005 to 2011 may affect the immunogenicity of the virus.

show abstract

“…A successful universal influenza vaccine will likely need to induce antibody and T-cell responses against multiple conserved antigens. To this end, we designed an LT adjuvanted, multi-antigen (LT-MA) DNA vaccine consisting of four HA genes, the ectodomain of M2 (M2e) and the consensus sequence from the highly conserved influenza A nucleoprotein (NP) [ 33 ]. The M2e gene is a 23 amino acid conserved B cell epitope that is poorly immunogenic [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

Multigenic DNA vaccine induces protective cross-reactive T cell responses against heterologous influenza virus in nonhuman primates

et al. 2017

View full text Add to dashboard Cite

Recent avian and swine-origin influenza virus outbreaks illustrate the ongoing threat of influenza pandemics. We investigated immunogenicity and protective efficacy of a multi-antigen (MA) universal influenza DNA vaccine consisting of HA, M2, and NP antigens in cynomolgus macaques. Following challenge with a heterologous pandemic H1N1 strain, vaccinated animals exhibited significantly lower viral loads and more rapid viral clearance when compared to unvaccinated controls. The MA DNA vaccine induced robust serum and mucosal antibody responses but these high antibody titers were not broadly neutralizing. In contrast, the vaccine induced broadly-reactive NP specific T cell responses that cross-reacted with the challenge virus and inversely correlated with lower viral loads and inflammation. These results demonstrate that a MA DNA vaccine that induces strong cross-reactive T cell responses can, independent of neutralizing antibody, mediate significant cross-protection in a nonhuman primate model and further supports development as an effective approach to induce broad protection against circulating and emerging influenza strains.

show abstract

Neutralizing antibody but not hemagglutination antibody provides accurate evaluation for protective immune response to H5N1 avian influenza virus in vaccinated rabbits

Cited by 10 publications

References 8 publications

Association Between Antibody Titers and Protection Against Influenza Virus Infection Within Households

Association Between Antibody Titers and Protection Against Influenza Virus Infection Within Households

Antigenic Variation in H5N1 clade 2.1 Viruses in Indonesia from 2005 to 2011

Multigenic DNA vaccine induces protective cross-reactive T cell responses against heterologous influenza virus in nonhuman primates

Contact Info

Product

Resources

About