Neutralizing Antibodies against Enteroviruses in Patients with Hand, Foot and Mouth Disease

Nguyet, Lam Anh; Thanh, Tran Tan; Thành, Nhân Lê; Hong, Nguyen Thi Thu; Nhu, Le Nguyen Truc; Van, Hoang Minh Tu; Ny, Nguyen Thi Han; Anh, Nguyen To; Han, Do Duong Kim; Tuan, Ha Manh; Vu, Huy Quang; Viet, Ho Lu; Cuong, Hoang Quoc; Thảo, Nguyễn Thị; Viet, Do Chau; Khanh, Truong Huu; Thwaites, Louise; Clapham, Hannah E.; Hưng, Nguyễn Thành; Châu, Nguyễn Văn Vĩnh; Thwaites, Guy; Ha, Do Quang; Doorn, H. Rogier van; Tan, Le Van

doi:10.3201/eid2602.190721

Cited by 12 publications

(8 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent evidence reported on recurrent HFMD episodes resulting from heterotypic reinfection in and lack of cross-neutralization among these four enterovirus serotypes observed in vaccine studies [ 37 – 40 ]. In the previous study showed that seropositive rates for heterotypic viruses (EV-A71, CV-A6, CV-A16), while at follow-up, were only a small proportion (3%–23%) of the patients had seroconverted for heterotypic viruses [ 41 ], which was higher than our study. However, it indicated that EV-A71, CV-A6, and CV-A16 cross-neutralization is absent or occurs in only a limited proportion of patients [ 42 , 43 ].…”

Section: Discussioncontrasting

confidence: 80%

“…However, it indicated that EV-A71, CV-A6, and CV-A16 cross-neutralization is absent or occurs in only a limited proportion of patients [ 42 , 43 ]. But, it cannot be ruled out that these rates of seropositive household contacts and seroconversion are due to prior exposure or coinfection with other serotypes that PCR may not have identified [ 41 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Incidence of Infection of Enterovirus 71 and Coxsackieviruses A6 and A16 among Household Contacts of Index Cases in Dong Thap Province, Southern Vietnam

Hoang

Nguyen

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

Background. Scarce information exists about immunity to hand, foot, and mouth disease (HFMD) among household contacts of index cases in Vietnam and what that means for reducing ongoing HFMD transmission in the community. Methods. We analyzed neutralizing antibodies (NT) and the incidence of enterovirus (EVs) infection among household contacts of index cases in a province where HFMD remains endemic. Throat swab and 2 mL blood samples from household contacts were collected at enrollment, during and after 2 weeks follow-up. Results. The incidence of EV-A71 infection among household contacts was 40/84 (47.6%, 95% Cl: 36.9-58.3%), compared with 106/336 (31.5%, 95% Cl: 26.6-36.5%) for CV-A6 and 36/107 (33.6%, 95% Cl: 24.7-42.6%) for CV-A16. The incidence of CV-A6 infection was fairly constant across ages; in contrast, CV-A71 and CV-A16 had some variation across ages. At baseline, higher geometric mean titer (GMT) of EV-A71, CV-A6, and CV-A16 antibody titers was found for 25-34-year groups (range 216.3 to 305.0) compared to the other age groups. There was a statistically significant difference in GMT values of CV-A6 and CV-A16 between those who had an infection or did not have infection among households with an index case of these serotypes. Conclusions. Our results indicated that adults were becoming infected with HFMD and could be contributing to the transmission. There is, therefore, a need for considering the household setting as an additional target for intervention programs for HFMD.

show abstract

Section: Discussioncontrasting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Incidence of Infection of Enterovirus 71 and Coxsackieviruses A6 and A16 among Household Contacts of Index Cases in Dong Thap Province, Southern Vietnam

Hoang

Nguyen

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…HFMD is a common clinical condition mostly caused by EV infection, and children with the disease usually develop a skin rash at the end of the hands and feet, and some even have oral ulcers, fever, and other clinical symptoms, which seriously affect their daily life. Although a large proportion of children with HFMD are less symptomatic and can recover after systematic therapeutic intervention, some who develop into severe HFMD with extremely rapid progression have a higher likelihood of developing complications such as pulmonary edema and meningitis, and the risk of death is substantially higher if no interventional therapeutic intervention is performed [ 20 – 22 ].…”

Section: Discussionmentioning

confidence: 99%

Application Effect of Medical Care Integration Combined with Family Intervention under the Evidence-Based Nursing Mode on Child Patients with Severe Hand-Foot-Mouth Disease and Its Influence on Intestinal Function

Zhang

Dong

Han

et al. 2021

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Objective. To explore the application effect of medical care integration combined with family intervention under the evidence-based nursing mode on child patients with severe hand-foot-mouth disease (HFMD) and its influence on intestinal function. Methods. 120 child patients with severe HFMD admitted to Qilu Children’s Hospital of Shandong University from January 2019 to January 2020 were selected as the research object and randomly divided into group A and group B, with 60 cases each. Conventional nursing was performed on patients in group B, and medical care integration combined with family intervention under the evidence-based nursing mode was performed on patients in group A. Patients were assessed after the intervention using the hospital-made treatment adherence scale, PedsQLTM 4.0 (Pediatric Quality of Life Inventory Version 4.0) scale, and the faces pain scale-revised (FPS-R). The levels of gastrointestinal function indicators such as serum endotoxin (ET), diamine oxidase (DAO), and d-lactic acid (D-LA) were measured before and after the intervention, and recovery such as time to clear fever and time to relief of oral pain were recorded in both groups. Results. Children in group A had significantly higher compliance in diet, behaviour, exercise, and medication than group B ( P < 0.05 ); the time to clear fever, time to relief of oral pain, time to healing of oral ulcers, time to relief of skin herpes, time to hospitalization, and time to eating were shorter in group A than those in group B ( P < 0.001 ); all scores on the PedsQLTM 4.0 scale were higher in group A than those in group B after the intervention ( P < 0.001 ); ET, DAO, and D-LA levels decreased in both groups after the intervention, with group A having lower levels than group B ( P < 0.001 ), in addition, group A had lower eating pain scores after the intervention ( P < 0.05 ). Conclusion. Medical care integration combined with family intervention under the evidence-based nursing mode can effectively improve the treatment compliance of child patients with severe HFMD, accelerate their recovery progress, ensure a better prognostic quality of life and gastrointestinal tract function, and reduce the eating pain, indicating that such comprehensive nursing intervention mode should be promoted in practice.

show abstract

“…(GMT=79.5) and Nguyet, L. A, at al. (GMT=295.1), which might be due to many reasons such as study design, different experimental setup and challenge strains, NAb titres are not always easy to compare between laboratories [ 13 , 16 ]. Nguyet, L. A, at al.…”

Section: Discussionmentioning

confidence: 99%

Kinetics of the neutralising antibody response in patients with hand, foot, and mouth disease caused by EV-A71: A longitudinal cohort study in Zhengzhou during 2017-2019

et al. 2021

View full text Add to dashboard Cite

Background Hand, foot, and mouth disease (HFMD) caused by enterovirus A71 (EV-A71) poses a serious threat to children's health. Kinetics of the neutralising antibody (NAb) response in EV-A71 infected HFMD patients remains unclear. The ideal sampling time of paired serum samples for serological diagnosis of EV-A71 infection is not well defined. Methods HFMD inpatients admitted to Henan Children's Hospital between February 15, 2017 and February 15, 2018 were enrolled. Serial serum samples collected during hospitalisation and up to 1.5 years after discharge were tested for NAb against EV-A71. Random intercept modelling with B-spline was conducted to characterize the kinetics of the EV-A71 NAb response over time after illness onset. Findings A total of 524 serum samples from 264 EV-A71 RNA positive HFMD inpatients were collected. NAb titres of EV-A71 infected patients were estimated to increase from 40 (95% CI: 9-180) at the day of onset to the peak of 2417 (95% CI: 1859-3143) at day 13, then remained above 1240 until 26 months. For serological diagnosis of EV-A71 infection, if at least a 4-fold rise in titre was used as the criteria, the acute phase serum should be collected at 0-4 days, the corresponding convalescent serum should be collected 14.9 days (95% CI: 9.1-23.8) after illness onset. Interpretation EV-A71 infection induced a strong and persistent humoral immune response in HFMD patients. The findings provide a scientific support for determining the collection time of paired serum samples for serological diagnosis of EV-A71 infected HFMD patients. Funding National Science Fund for Distinguished Young Scholars

show abstract

Neutralizing Antibodies against Enteroviruses in Patients with Hand, Foot and Mouth Disease

Cited by 12 publications

References 28 publications

Incidence of Infection of Enterovirus 71 and Coxsackieviruses A6 and A16 among Household Contacts of Index Cases in Dong Thap Province, Southern Vietnam

Incidence of Infection of Enterovirus 71 and Coxsackieviruses A6 and A16 among Household Contacts of Index Cases in Dong Thap Province, Southern Vietnam

Application Effect of Medical Care Integration Combined with Family Intervention under the Evidence-Based Nursing Mode on Child Patients with Severe Hand-Foot-Mouth Disease and Its Influence on Intestinal Function

Kinetics of the neutralising antibody response in patients with hand, foot, and mouth disease caused by EV-A71: A longitudinal cohort study in Zhengzhou during 2017-2019

Contact Info

Product

Resources

About