Neurotrophic function of conditioned medium from human amniotic epithelial cells

Uchida, S.; Inanaga, Yukiko; Kobayashi, Makiko; Hurukawa, Shoei; Araie, Makoto; Sakuragawa, Norio

doi:10.1002/1097-4547(20001115)62:4<585::aid-jnr13>3.0.co;2-u

Cited by 139 publications

(65 citation statements)

References 20 publications

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“…The observed therapeutic effects are probably mediated by secretion of diffusible factors, including neurotransmitters (Elwan & Sakuragawa 1997;Sakuragawa et al 1997Sakuragawa et al , 2001) and neurotrophic and growth factors (Koizumi et al 2000;Uchida et al 2000).…”

Section: Brainmentioning

confidence: 98%

Biological characteristics of stem cells from foetal, cord blood and extraembryonic tissues

Abdulrazzak

Moschidou

Jones

et al. 2010

J. R. Soc. Interface.

125

115

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Foetal stem cells (FSCs) can be isolated during gestation from many different tissues such as blood, liver and bone marrow as well as from a variety of extraembryonic tissues such as amniotic fluid and placenta. Strong evidence suggests that these cells differ on many biological aspects such as growth kinetics, morphology, immunophenotype, differentiation potential and engraftment capacity in vivo. Despite these differences, FSCs appear to be more primitive and have greater multi-potentiality than their adult counterparts. For example, foetal blood haemopoietic stem cells proliferate more rapidly than those found in cord blood or adult bone marrow. These features have led to FSCs being investigated for pre-and post-natal cell therapy and regenerative medicine applications. The cells have been used in pre-clinical studies to treat a wide range of diseases such as skeletal dysplasia, diaphragmatic hernia and respiratory failure, white matter damage, renal pathologies as well as cancers. Their intermediate state between adult and embryonic stem cells also makes them an ideal candidate for reprogramming to the pluripotent status.

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Section: Brainmentioning

confidence: 98%

Biological characteristics of stem cells from foetal, cord blood and extraembryonic tissues

Abdulrazzak

Moschidou

Jones

et al. 2010

J. R. Soc. Interface.

125

115

View full text Add to dashboard Cite

show abstract

“…Pioneering research by Sakuragawa et al (1996) demonstrated not only that hAEC express markers of glial and neuronal progenitor cells, but also perform multiple neuronal functions such as synthesis and release of acetylcholine, catecholamines, neurotrophic factors, activin and noggin Koyano et al 2002;Sakuragawa et al 1997;Uchida et al 2000). As well as performing functions that are characteristic of neuronal cells, other studies report evidence that cells of the amniotic epithelium also provide support for growth of neuronal cell types in vitro.…”

Section: Human Amniotic Epithelial Cellsmentioning

confidence: 99%

“…As well as performing functions that are characteristic of neuronal cells, other studies report evidence that cells of the amniotic epithelium also provide support for growth of neuronal cell types in vitro. hAEC conditioned medium has been shown to have neurotrophic effects on rat cortical cells (Uchida et al 2000) and support the survival of chicken neural retinal cells (Tcheng et al 1994), while another study has shown that chick dorsal root ganglia are able to survive and grow on human amniotic membrane in the absence of neurotrophic factors, and it appears that these effects may be mediated at least in part by the amniotic epithelial layer (Schroeder et al 2007). …”

Section: Human Amniotic Epithelial Cellsmentioning

confidence: 99%

Placenta-derived stem cells: new hope for cell therapy?

2008

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An urgent current need in regenerative medicine is that of identifying a plentiful, safe and ethically acceptable stem cell source for the development of therapeutic strategies to restore functionality in damaged or diseased organs and tissues. In this context, human term placenta represents a prime candidate, as it is available in nearly unlimited supply, is ethically problem-free and easily procured. Placental cells display differentiation capacity toward all three germ layers, while also displaying immunomodulatory effects, therefore supporting the possibility that they could be applied in an allogeneic transplantation setting. Although promising data have been reported to date, further study is required to fully characterize the differentiation potential of placenta-derived cells and to identify their possible clinical applications. Here, we provide a snapshot of current knowledge regarding the potential of cells from the amniotic membrane of human term placenta to address current shortcomings in the field of regenerative medicine.

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“…HAECs lack major histocompatibility complex (MHC) antigen; therefore, there is a low risk for tissue rejection following transplantation [9]. HAECs secrete biologically active neurotrophins, such as brain-derived neurotrophic factor (BDNF), neurotrophin-3, insulin-like growth factors, epidermal growth factor, prostaglandin E2, laminin, nerve growth factor (NGF), and transforming growth factor-alpha [10,11], some of which exhibit trophic activities on dopaminergic neurons following transplantation [12]. In addition, HAECs do not express telomerase and are non-tumorigenic.…”

mentioning

confidence: 99%

Therapeutic effects of human amniotic epithelial cell transplantation on double-transgenic mice co-expressing APPswe and PS1ΔE9-deleted genes

Xue

Chen

Dong

et al. 2012

Sci. China Life Sci.

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Human amniotic epithelial cells (HAECs), which exhibit characteristics of embryonic and pluripotent stem cells, could be utilized for cell therapy without legal or ethical problems. Double-transgenic (TG) mice (n=20) and wild-type (WT) mice (n=20) were randomly assigned to two groups, respectively. The transplantation group was treated with HAECs and the control group with PBS. A six-radial arm water maze was used to assess spatial memory. Immunofluorescence was utilized to track HAEC survival. Immunohistochemistry was used to determine octamer-binding protein 4 (oct-4) and nanog expression in the HAECs. High-performance liquid chromatography (HPLC) was used to measure acetylcholine levels in the hippocampus. The density of cholinergic neurons in the basal forebrain and nerve fibers in the hippocampus was measured following acetylcholinesterase staining. Results showed that transplanted HAECs survived for at least eight weeks and migrated to the third ventricle without immune rejection. Graft HAECs also expressed the specific stem cell markers oct-4 and nanog. Compared with the control group, HAEC transplantation significantly ameliorated spatial memory deficits in TG mice, as well as increased acetylcholine levels and the number of hippocampal cholinergic neurites. Intracerebroventricular HAEC transplantation improved spatial memory in double-TG mice, and results suggested that increased acetylcholine levels in the hippocampus, released by surviving cholinergic neurites, were responsible for this improvement.Alzheimer's disease, human amniotic epithelial cells, spatial memory deficit, transgenic mice Citation:Xue S R, Chen C F, Dong W L, et al. Therapeutic effects of human amniotic epithelial cell transplantation on double-transgenic mice co-expressing APPswe and PS1E9-deleted genes.

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Neurotrophic function of conditioned medium from human amniotic epithelial cells

Cited by 139 publications

References 20 publications

Biological characteristics of stem cells from foetal, cord blood and extraembryonic tissues

Biological characteristics of stem cells from foetal, cord blood and extraembryonic tissues

Placenta-derived stem cells: new hope for cell therapy?

Therapeutic effects of human amniotic epithelial cell transplantation on double-transgenic mice co-expressing APPswe and PS1ΔE9-deleted genes

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