Neurotransmitter synthesis, storage, and turnover in neonatally deafferented sympathetic neurons

Smolen, Arnold J.; Beaston-Wimmer, Patricia; Wright, Linda L.; Lindley, Theresa A.; Cader, Cas

doi:10.1016/0165-3806(85)90043-4

Cited by 25 publications

(9 citation statements)

References 15 publications

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“…Unoperated littermates served as controls. Previous studies (data not shown) have indicated that SV levels in sham-operated animals do not differ from those in unoperated controls (see also Smolen et al, 1985, for similar observations). All surgery was carried out bilaterally to reduce the number of animals subjected to surgery and to circumvent possible compensatory reactions in the contralateral SCG (cf.…”

Section: Methodssupporting

confidence: 72%

“…The rodent superior cervical ganglion (SCG) has been used extensively as a model system in which to study postnatal neural development. For example, tyrosine hydroxylase (TH), the rate-limiting enzyme in catecholamine biosynthesis, undergoes a significant increase during the second postnatal week (Black et al, 1971;Smolen et al, 1985). Synaptogenesis, as monitored both by morphological criteria (Black et al, 1971;Smolen and Raisman, 1980) and by assay of choline-acetyltransferase (Black et al, 1971), occurs largely after birth and is essentially complete by 30 days.…”

Section: Introductionmentioning

confidence: 99%

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Neonatal deafferentation prevents normal expression of synaptic vesicle antigens in the developing rat superior cervical ganglion

Greif¹,

Trenchard²

1988

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The effect of neonatal deafferentation of the rat superior cervical ganglion (SCG) on the expression of two synaptic vesicle proteins was studied to assess the role of transsynaptic influences in the regulation of these neural antigens in the SCG. The two proteins studied were a 65 kilodalton integral membrane protein of synaptic vesicles (SV), and synapsin-1 (S-1), a synaptic vesicle phosphoprotein. Antigen levels were quantified by radioimmunoassay using antibodies directed against the proteins. Distribution of SV in control, deafferented and reinnervated ganglia from 30-day-old rats was visualized by immunohistochemical labeling. Levels of both antigens were reduced following deafferentation of the SCG on postnatal days 1-3. The reduction in S-1 levels at 30 days was less than that observed for SV. The amount of S-1 remaining in deafferented ganglia was consistent with estimates of the postsynaptic pool in the SCG reported previously. SV levels, in contrast, were reduced to 24% of control levels, suggesting that SV synthesis in principal ganglionic neurons might be affected. The time course of postnatal development of S-1 in the SCG differed from previous studies of SV expression, with significant increases occurring after the second week after birth. The differences in response to deafferentation may reflect functional differences of the two vesicle-associated proteins. These studies demonstrate that transsynaptic regulation of antigens other than those directly associated with neurotransmitters occurs in the SCG.

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Section: Methodssupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Neonatal deafferentation prevents normal expression of synaptic vesicle antigens in the developing rat superior cervical ganglion

Greif¹,

Trenchard²

1988

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“…Reductions in total protein per ganglion following neonatal deafferentation are consistent with previous studies [2,10]. SCGs deprived of preganglionic innervation from birth contain less protein at all ages examined, with the greatest reduction from control levels at 14 days.…”

Section: :Jcb Greif and Trenchardsupporting

confidence: 90%

Evidence for transsynaptic regulation of neuronal cell surface heparan sulfate proteoglycan in developing rat superior cervical ganglion

Greif¹,

Trenchard²

1984

J of Cellular Biochemistry

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The effect of neonatal deafferentation on the expression of a neuronal cell surface heparan sulfate proteoglycan (HeS-PG) was investigated in the developing rat superior cervical ganglion. Two monoclonal antibodies, one directed against the core protein of HeS-PG, and one to a determinant associated with a heparan sulfate side-chain, were used to monitor postnatal increases of HeS-PG by radioimmunoassay. Following neonatal deafferentation by section of the cervical sympathetic trunk, total protein per ganglion was slightly reduced at survival times of 7, 14, and 30 days. Expression of the core protein determinant on HeS-PG was not altered in deafferented ganglia. In contrast, levels of side-chain determinant were significantly reduced at 14 and 30 days. These results suggest that processing of HeS-PG side-chains by principal ganglionic neurons is partially regulated by transsynaptic influences during development. Transsynaptic regulation of neuronal development may be a more general process than was believed previously, with effects not limited to molecules associated with synaptic development.

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“…Ryan (1990), in reviewing several controlled trials of tricyclic antidepressants in adolescent MDD, demonstrated that nortriptyline, amitriptyline and imipramine were no more effective than placebo in alleviating depression. One of his conclusions was that trials using serotonergic antidepressants need to be conducted, particularly since there is evidence in animals that the noradrenergic system does not fully develop anatomically and functionally until early adulthood (Black, Hendry and Iversen, 1971;Goldman-Rakic and Brown, 1982;Smolen et al, 1985;Thoenen, Kettler and Saner, 1972). This suggests that primarily noradrenergic antidepressants may not be effective until adulthood is reached.…”

Section: Introductionmentioning

confidence: 99%

Fluvoxamine in the treatment of depressive illness in children and adolescents

Cosgrove¹

1994

J Psychopharmacol

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Neurotransmitter synthesis, storage, and turnover in neonatally deafferented sympathetic neurons

Cited by 25 publications

References 15 publications

Neonatal deafferentation prevents normal expression of synaptic vesicle antigens in the developing rat superior cervical ganglion

Neonatal deafferentation prevents normal expression of synaptic vesicle antigens in the developing rat superior cervical ganglion

Evidence for transsynaptic regulation of neuronal cell surface heparan sulfate proteoglycan in developing rat superior cervical ganglion

Fluvoxamine in the treatment of depressive illness in children and adolescents

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