Evidence for transsynaptic regulation of neuronal cell surface heparan sulfate proteoglycan in developing rat superior cervical ganglion

Greif, Karen F.; Trenchard, Holly

doi:10.1002/jcb.240260208

Cited by 8 publications

(3 citation statements)

References 18 publications

(19 reference statements)

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“…We have demonstrated previously that neonatal deafferentation of the SCG alters the expression of a neuronal cell surface heparan sulfate proteoglycan (Greif and Trenchard, 1984a). A selective depression in the levels of a n antigenic determinant on the carbohydrate side chain is observed, with no alterations in the levels of a determinant associated with the core protein.…”

Section: Discussionmentioning

confidence: 90%

“…peroxidase (HRP)-conjugated antimouse Ig and protein Bar = 10 pm. Days after Surgery was carried out according to published procedures (Smolen and Raisman, 1980;Greif and Trenchard, 1984a). Pups aged 1-3 days were removed as a litter, and equal numbers of males and females were placed in each experimental and control group.…”

Section: Methodsmentioning

confidence: 99%

“…The assay used is a n estimate of the dilution of whole tissue homogenate required to inhibit 50% of antibody binding in a standard solid-phase radioimmunoassay (RIA; Greif and Reichardt, 1982;Greif and Trenchard, 1984a). Animals were killed by decapitation, and SCGs were removed immediately and placed in phosphatebuffered saline (PBS) with protease inhibitors.…”

Section: Radioimmunoassaymentioning

confidence: 99%

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Neonatal deafferentation prevents normal expression of synaptic vesicle antigens in the developing rat superior cervical ganglion

Greif¹,

Trenchard²

1988

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The effect of neonatal deafferentation of the rat superior cervical ganglion (SCG) on the expression of two synaptic vesicle proteins was studied to assess the role of transsynaptic influences in the regulation of these neural antigens in the SCG. The two proteins studied were a 65 kilodalton integral membrane protein of synaptic vesicles (SV), and synapsin-1 (S-1), a synaptic vesicle phosphoprotein. Antigen levels were quantified by radioimmunoassay using antibodies directed against the proteins. Distribution of SV in control, deafferented and reinnervated ganglia from 30-day-old rats was visualized by immunohistochemical labeling. Levels of both antigens were reduced following deafferentation of the SCG on postnatal days 1-3. The reduction in S-1 levels at 30 days was less than that observed for SV. The amount of S-1 remaining in deafferented ganglia was consistent with estimates of the postsynaptic pool in the SCG reported previously. SV levels, in contrast, were reduced to 24% of control levels, suggesting that SV synthesis in principal ganglionic neurons might be affected. The time course of postnatal development of S-1 in the SCG differed from previous studies of SV expression, with significant increases occurring after the second week after birth. The differences in response to deafferentation may reflect functional differences of the two vesicle-associated proteins. These studies demonstrate that transsynaptic regulation of antigens other than those directly associated with neurotransmitters occurs in the SCG.

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Section: Discussionmentioning

confidence: 90%

Section: Methodsmentioning

confidence: 99%

Section: Radioimmunoassaymentioning

confidence: 99%

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