Neuroprotective properties of n-phenylacetyl-l-prolylglycine ethyl ester nasal gel in an experimental model of multiple sclerosis equivalent

Burlaka, B. S.; Бєленічев, Ігор Федорович; Nefedov, O. O.; Aliyeva, Olena G.; Bukhtiyarova, N. V.

doi:10.26641/2307-0404.2020.4.221226

Cited by 2 publications

(3 citation statements)

References 2 publications

(1 reference statement)

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“…Images of neurons obtained on a microscope using a highly sensitive video camera COHU-4922 (COCHU Inc., USA) were entered into a computer hardware-software system for digital image analysis VIDAS. Image analysis was performed in a semi-automatic mode (Burlaka et al 2021).…”

Section: Resultsmentioning

confidence: 99%

“…Due to the fact that considerable attention is paid to various methods of treating demyelinating pathology of the CNS, it becomes especially important to have a well-formed experimental pathological model that reproduces demyelination in the central nervous system, which is most similar to the manifestations of multiple sclerosis (MS) in humans (Browne et al 2014;Pichkur et al 2017;Burlaka et al 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Animal models, such as experimental autoimmune (allergic) encephalomyelitis (EAE), are widely used to investigate potential therapeutic agents for MS (Constantinescu et al 2011;Dobson and Giovannoni 2019). While sensitization to autoantigens naturally occurs by unknown mechanisms, the EAE model requires external immunization (Gran et al 2007;Belenichev et al 2015;Burlaka et al 2020). EAE is an organ-specific cell-mediated autoimmune demyelinating disease of the CNS in which macrophages and T-lymphocytes mediate damage to the myelin sheath.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of methods of modeling and formation of experimental allergic encephalomyelitis

Nefodov¹,

Бєленічев

Fedchenko³

et al. 2022

RRP

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Introduction: Experimental autoimmune (allergic) encephalomyelitis (EAE) induced by intradermal injection of homogenate of the brain, spinal cord and peripheral nerve with Freund’s stimulator, refers to a true autoimmune disease of the nervous system. Materials and methods: Experimental studies were conducted on white nonlinear rats. To induce experimental allergic encephalomyelitis (EAE), homologous brain homogenates was used, which leads among other drugs (homologous, heterogeneous brain and spinal cord homogenates) by encephalitogenity. The connective tissue of the animal’s tail base was injected with a mixture of encephalitogenic suspension of 0.1 ml per 100 g of the body weight. Results and discussion: According to the results, in the rats, there was weight loss, and the abnormal neurological symptoms were found on an average of 10–12th days. Our experimental studies on the formation of EAE were confirmed morphologically by electron microscopy. Conclusion: Thus, the use of this technique allowed us to obtain a simulated pathologic condition of multiple sclerosis in the form of experimental allergic encephalomyelitis and can be used in future studies to identify appropriate laws, the extent and nature of changes in the immune and nervous systems of the body when inducing experimental pathological conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%