Neuroprotective effect of s-methylisothiourea in transient focal cerebral ischemia in rat

Arunadevi, R.; Ramteke, Vinod D.; Kumar, Saurabh; Shukla, Manoj K.; Subramani, Jaganathan; Kumar, Dinesh; Sharma, Anil Kumar; Tandan, Surendra K.

doi:10.1016/j.niox.2009.10.002

Cited by 37 publications

(21 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the inhibition of iNOS, which is responsible for the synthesis of nitric oxide, may be a therapeutic option for preventing pulmonary fi brosis. SMT is a relatively selective iNOS inhibitor and its therapeutic effi cacy has been demonstrated in many animal studies (24)(25). Guo et al treated mice with induced herpes simplex encephalitis with SMT, and reported that that was just due to inhibition of pro-infl ammatory cytokines such as TNF-α, IL-1β, NO (26).…”

Section: Discussionmentioning

confidence: 99%

Attenuation of bleomycin induced lung fibrosis by erdosteine and inhibition of the inducible nitric oxide synthase

Kayhan¹,

Tütüncü²,

Güzel³

et al. 2015

BLL

View full text Add to dashboard Cite

Background and objectives: Despite advances in treatment modalities, the discovery of optimal medical therapies still remains a necessity in the management of pulmonary fi brosis. Material and methods:The experiments were performed in 35 adult Sprague Dawley rats, randomly allotted into one of fi ve groups (n=7). The control group was treated with 1 ml/kg, 0.9 % saline; the BLM group was given a single dose of BLM (2.5 U/kg); the BLM+ER group was treated with ER (10 mg/kg/day po) for 14 days after BLM administration; the BLM+SMT group was treated with i.p injections of SMT (20 mg/kg/ day) for 14 days after BLM administration; the BLM+ER+SMT group was treated with ER and SMT for 14 days after BLM administration. At the end of day 14, the results of histopathological, biochemical, and immunohistochemical investigations were analyzed. Results: Serum TNF-α, nitrate/nitrite, and TBARS levels signifi cantly increased in BLM group compared to control group (p < 0.001, p < 0.001 and p < 0.05 respectively). Lung tissue content of IL-6 was found to be lower in BLM+ER, BLM+SMT and BLM+ER+SMT groups compared to BLM group by immunhistochemical examinations (p < 0.01, p < 0.01 and p < 0.001, respectively). Similarly, the TNF-α reactions (p < 0.01 for each group) and NF-kB expressions were shown to be signifi cantly different among the study groups (p < 0.05, p < 0.05 and p < 0.001, respectively). Conclusion: Based on our study, ER and SMT attenuate BLM-induced pulmonary fi brosis; the combination of two agents has a greater protective effi cacy against fi brosis than one alone, reducing the infl ammatory markers (Tab. 2, Fig. 2, Ref. 31). Text in PDF www.elis.sk.

show abstract

Section: Discussionmentioning

confidence: 99%

Attenuation of bleomycin induced lung fibrosis by erdosteine and inhibition of the inducible nitric oxide synthase

Kayhan¹,

Tütüncü²,

Güzel³

et al. 2015

BLL

View full text Add to dashboard Cite

show abstract

“…Nitrosative stress is term used to describe the increased production of RNS that nitrosylate and alter protein structure and function (Ridnour et al 2004). In brain stroke, NO produced by nNOS and iNOS can be neurotoxic (Huang et al 1994;Zhao et al 2000), partly as a consequence of formation peroxynitrite free radical, which leads to direct damage to mitochondrial enzyme and DNA (Sims and Anderson 2002;ArunaDevi et al 2010). In contrast, NO produced by eNOS is beneficial in brain stroke Endres et al 1998).…”

Section: Discussionmentioning

confidence: 99%

Damage to Neurons and Oligodendrocytes in the Hippocampal CA1 Sector after Transient Focal Ischemia in Rats

et al. 2010

View full text Add to dashboard Cite

Focal brain lesions such as transient focal cerebral ischemia can lead to neuronal damage in remote areas, including the ipsilateral substantia nigra and hippocampus, as well as in the ischemic core. In this study, we investigated acute changes in the ipsilateral hippocampus from 1 up to 7 days after 90 min of transient focal cerebral ischemia in rats, using anti-NeuN (neuronal nuclei), anti-Cu/Zn-superoxide dismutase (Cu/Zn-SOD), anti-Mn-SOD, anti-neuronal nitric oxide synthase (nNOS), anti-inducible NOS (iNOS), anti-glial fibrillary acidic protein (GFAP), anti-ionized calcium-binding adaptor molecule 1(Iba 1) and anti-2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) antibodies. In our western blot and histochemical analyses, present results show that transient focal cerebral ischemia in rats can cause a severe and acute damage of neurons and oligodendrocytes in the ipsilateral hippocampal CA1 sector. The present findings also demonstrate that the expression of iNOS produced by Iba 1-immunopositive microglia precedes the damage of neurons and oligodendrocytes in the ipsilateral hippocampal CA1 sector after transient focal cerebral ischemia. In contrast, our results suggest that increased reactive oxygen species (ROS) production during reperfusion cannot lead to damage of neurons and oligodendrocytes in the ipsilateral hippocampal CA1 sector after transient focal cerebral ischemia, because of an insufficient expression of Cu/Zn-SOD and Mn-SOD. Our double-labeled immunohistochemical study demonstrates that the overexpression of iNOS produced by Iba 1-immunopositive microglia may play a pivotal role in the damage of neurons and oligodendrocytes in the ipsilateral hippocampal CA1 sector at an acute stage after transient focal cerebral ischemia.

show abstract

“…S-methylisothiourea (SMT) is not a selective iNOS inhibitor and reduces plasma NO levels. But it is 10-to 20-fold more potent than NG methyl-Larginine or other iNOS inhibitors known [21].…”

Section: Introductionmentioning

confidence: 95%

Protective Effects of Melatonin and S-Methylisothiourea on Mechlorethamine Induced Nephrotoxicity

Kunak

Macit

Yaren

et al. 2012

Journal of Surgical Research

View full text Add to dashboard Cite

Neuroprotective effect of s-methylisothiourea in transient focal cerebral ischemia in rat

Cited by 37 publications

References 69 publications

Attenuation of bleomycin induced lung fibrosis by erdosteine and inhibition of the inducible nitric oxide synthase

Attenuation of bleomycin induced lung fibrosis by erdosteine and inhibition of the inducible nitric oxide synthase

Damage to Neurons and Oligodendrocytes in the Hippocampal CA1 Sector after Transient Focal Ischemia in Rats

Protective Effects of Melatonin and S-Methylisothiourea on Mechlorethamine Induced Nephrotoxicity

Contact Info

Product

Resources

About