Neuroprotective effect of quercetin in ectoenzymes and acetylcholinesterase activities in cerebral cortex synaptosomes of cadmium-exposed rats

Abstract

This study investigated the effect of quercetin on nucleoside triphosphate diphosphohydrolase (NTP-Dase), 50-nucleotidase, adenosine deaminase (ADA), and acetylcholinesterase (AChE) activities in synaptosomes from cerebral cortex of adult rats exposed to cadmium (Cd). Rats were exposed to Cd (2.5 mg/Kg) and quercetin (5, 25 or 50 mg/Kg) by gavage for 45 days. Rats were randomly divided into eight groups (n = 8-10): saline/ethanol, saline/Querc 5 mg/kg, saline/Querc 25 mg/kg, saline/Querc 50 mg/kg, Cd/ethanol, Cd/Querc 5 mg/kg, Cd/Querc 25 mg/kg, and Cd/Querc 50 mg/kg. Results demonstrated that AChE activity increased in the Cd/ethanol group when compared to saline/ethanol group. Treatment with quercetin prevented the increase in AChE activity when compared to Cd/ethanol group. Quercetin treatment prevented the cadmium-induced increase in NTPDase, 5-nucleotidase, and ADA activities in Cd/ethanol group when compared to saline/ethanol group. Our data showed that quercetin have a protector effect against Cd intoxication. This way, is a promising candidate among the flavonoids to be investigated as a therapeutic agent to attenuate neurological disorders associated with Cd intoxication.

Cytoprotective effects of dietary flavonoids against cadmium‐induced toxicity

“…However, the specific intervention mechanism needs further study. Treatment with quercetin (5, 25, or 50 mg/kg) and Cd (2.5 mg/kg) reduced NTPDase, 5′‐nucleotidase, ADA, and AChE activity in synaptosomes from the cerebral cortex of adult rats exposed to Cd . However, quercetin totally or partially prevented oxidative stress and increased AChE and Na + ,K + ‐ATPase activities in the cerebral cortex, hippocampus, and hypothalamus of rats exposed to Cd, preventing memory impairment and anxiogenic‐like behavior (Fig.…”

“…found that Cd‐impaired memory and neurological disorders were associated with decreased activity of AChE and the Na + ,K + ‐ATPase in the cerebral cortex and the hippocampus of Cd‐exposed rats. AChE activity increased in synaptosomes from the cerebral cortex of adult rats exposed to Cd . ATP or ADP can be hydrolyzed by nucleoside triphosphate diphosphohydrolase (NTPDase) to form Aronia melanocarpa berry (AMP), as its hydrolysis contributes to adenosine formation in the presence of 5′‐nucleotidase.…”

“…Deaminase (ADA) hydrolyzes adenosine to form inosine. Abdalla et al . found that NTPDase, 5′‐nucleotidase, and ADA activities increased in adult rats receiving Cd treatment by gavage …”

“…AChE activity increased in synaptosomes from the cerebral cortex of adult rats exposed to Cd. 40 ATP or ADP can be hydrolyzed by nucleoside triphosphate diphosphohydrolase (NTPDase) to form Aronia melanocarpa berry (AMP), as its hydrolysis contributes to adenosine formation in the presence of 5 -nucleotidase. Deaminase (ADA) hydrolyzes adenosine to form inosine.…”

Modulation of Key Biochemical Markers Relevant to Stroke by Antiaris Africana Leaf Extract Following Cerebral Ischemia/Reperfusion Injury

“…The hydrolytic enzyme, cholinesterase, regulates ACh levels by breaking down this neurotransmitter in many tissues. AChE activity is altered in response to redox imbalance and affected by various degree of injury to the organism which can stimulate rapid ACh degradation and consequently, decreased stimulation of ACh receptors leading to negative effects on cholinergic neurotransmission as well promotion of cognitive impairment (Elufioye et al, 2010;Abdalla et al, 2013 The neurotransmitter, dopamine, is produced from the amino acid, tyrosine, through the catalytic action of tyrosine hydroxlase (TH) which is the rate-limiting enzyme in the pathway. (Daubner and Wang, 2011).…”