Caffeic acid phenethyl ester mitigates cadmium‐induced damage via the Hsa_circ_0010039/miR‐661/Caspase9 axis–mediated apoptosis

Abstract

Caffeic acid phenethyl ester (CAPE) is a bioactive component of honeybee propolis, which has protective effect against heavy metal induced toxicology. Cadmium is a kind of heavy metal pollutant that is hazardous to human health especially liver, and apoptosis is an important mechanism related to cadmium injury. Circular RNAs (CircRNAs) are involved in the regulation of apoptosis. However, the protective effects of CAPE on cadmium-induced cell apoptosis through CircRNA related mechanisms remains unclear at the current stage. In the present research, cell-based studies revealed that treating HepG2 cells with CdCl 2 (0-30 μM; 24 h) caused a dose-dependent decrease in cell viability, and CAPE at 10 μM could reverse such a decrease. The circular RNA hsacirc-0010039 may function as a ceRNA for the microRNA miR-661 to enhance caspase 9 expression and regulate apoptosis. The anti-apoptotic effects of CAPE against CdCl 2induced injury may be achieved through inhibiting the expression of hsa-circ-0010039 and caspase 9 while up-regulating miR-661 expression. This is the first report on the involvement of circRNA in CAPE's alleviating effect on CdCl 2 -induced injury and apoptosis. The hsa_circ_0010039/miR-661/caspase9 axis deserves further research efforts especially in search of dietary interventions for cadmium injury.

Myricetin and dihydromyricetin as JNK1 inhibitors protect against ROS‐mediated oxidative stress and apoptosis

“…HepG2 cells were seeded in 96‐well plates. After the treatments with H 2 O 2 , myricetin, and dihydromyricetin, the cells were incubated with 10 μM 2’,7‐dichlorodihydro‐fluorescein diacetate (DCFH‐DA; Sigma Aldrich) for 20 min at 37°C in the dark, then subjected to fluorescence measurements using a microplate reader (absorption wavelength: 488 nm, excitation wavelength: 525 nm) (Bio‐Rad) (Hao et al, 2021b).…”

The antagonistic effect of caffeic acid phenethyl ester on cadmium‐caused pulmonary toxicity: MiR‐182‐5p/TLR4 axis

“…The protein levels of oxidative stress, apoptosis, and inflammation indicators in mice lung tissue were measured by Western blot (Hao, Ge, et al., 2021). Details and the information of the primary antibodies were supplied in Supporting Information section.…”

The interaction between miRNAs and hazardous materials

“… [ 122 ] CdCl2 miR-661 (Down) – – Caffeic acid phenethyl ester (CAPE); 10 μM HepG2 0–30 μM, 24 h Caspase-9 CAPE could downregulate apoptosis induced by CdCl2. [ 123 ] Pb miR-16-5p (Up) Hy-Line Brown chickens – Se; (1 μM of Na2SeO3) Neutrophil 12.5 μM IGF1R, PiK3R1, p53, Bcl-2, Bax, Caspase-3/8/9 In chicken neutrophil cells, Se via targeting miR-16-5p had an antagonistic impact against lead-induced apoptosis. [ 73 ] Pb miR-224 (Up) Male Wistar rats 30 mg/kg, once every 2 days, less than 4 months Selenium nanoparticles (Se-NPs); 0.5 mg/kg, less than 4 months – – ID1 Se-NPs via inhibiting miR-224 could attenuate adverse effects of Pb on thyroid tissues.…”