Neuroprotective effect of human mesenchymal stem cells in a compartmentalized neuronal membrane system

Piscioneri, Antonella; Morelli, Sabrina; Mele, Maria Cristina; Canonaco, Marcello; Bilotta, Eleonora; Pantano, Pietro; Drioli, Enrico; Bartolo, Loredana De

doi:10.1016/j.actbio.2015.06.013

Cited by 28 publications

(19 citation statements)

References 36 publications

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“…In that system, different cells can be cultured in upper and lower compartments, which are separated by a microporous membrane. Various examples of work by other groups have demonstrated the importance of separation of the target cell to be regenerated and its corresponding supporting cells [Piscioneri et al, 2015]. Those microporous membranes are made of materials such as PET, polycarbonate, and polytetrafluoroethylene, with a thickness of 10-20 μm and a pore size of 0.4-8 μm.…”

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confidence: 99%

An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration

Tseng¹,

Chew²,

Huang³

et al. 2017

Cells Tissues Organs

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Recently, a novel substrate known as an electrospun polylactic acid (PLLA) microtube array membrane (MTAM) was successfully developed as a cell coculture platform. Structurally, this substrate is made up of one-to-one connected, ultrathin, submicron scale fibers that are arranged in an arrayed formation. Its unique structure confers several key advantages which are beneficial in a cell coculture system. In this study, the interaction between rat fetal neural stem cells (NSC) and astrocytes was examined by comparing the outcome of a typical Transwell-based coculture system and that of an electrospun PLLA MTAM-based coculture system. Compared to tissue culture polystyrene (TCP) and Transwell coculture inserts, a superior cell viability of NSC was observed when cultured in lumens of electrospun PLLA MTAM (with supportive immunostaining images). Reverse transcription polymerase chain reaction revealed a strong interaction between astrocytes and NSC through a higher expression of doublecortin and a lower expression of nestin. These data demonstrate that MTAM is clearly a better coculture platform than the traditional Transwell system.

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mentioning

confidence: 99%

An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration

Tseng¹,

Chew²,

Huang³

et al. 2017

Cells Tissues Organs

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“…Transplantation of MSCs can make changes in the secreted cytokines and reduce the secretion of TNF-α, IL-1 and IL-20 (pre-inflammatory cytokines) and increase the TGF-β secretion which regulate inflammatory responses (Piscioneri et al, 2015). Another role of MSCs is making the microenvironment enrich for neural regeneration and proliferation of other cells.…”

Section: Discussionmentioning

confidence: 99%

Effect of Bone Marrow-derived Mesenchymal Stem Cells on Changes of Serum Levels of TNF-α and Locomotor Function after Spinal Cord Injury in Mice

Gashmardi¹,

Mehrabani²,

Khodabande³

et al. 2015

J. of Medical Sciences

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Spinal Cord Injury (SCI) is still a devastating clinical problem with irreversible consequences leading to permanent functional loss and life time disability. This study was conducted to assess the healing effect of bone marrow-derived mesenchymal stem cells on locomotor function and changes of Tumor Necrosis Factor alpha (TNF-α) after SCI in mice. Forty two BALB/C mice were divided into 3 equal groups of control, SCI and treatment [transplantation of 5×10 4 Bone Marrow Stem Cells (BMSCs)]. The SCI was induced by compression for 2 min at T10 and injury bilaterally. The femoral and tibial bones were used for bone marrow isolation and culture was made using Dulbecco's Modified Eagle's Medium supplemented with fetal bovine serum, L-glutamine and penicillin/streptomycin. Cell morphology was evaluated in all passages. Characterization of BMSCs was conducted by reverse transcription polymerase chain reaction and by osteogenic differentiation of BMSCs. The ELISA was undertaken for TNF-α. Open field locomotion was evaluated by Toyama mouse score. The BMSCs were plastic adherent and fibroblastic spindle-shape. MSCs were positive for CD90 and negative for CD34 and CD45. Osteogenic differentiation was noticed when stained with alizarin red. The serum TNF-α level increased after 24 h, 3 and 5 weeks post-SCI and was time dependent. The neurological score significantly improved after 8 weeks after BMSC transplantation. Transplantation of BMSCs was shown to decrease the TNF-α level and inflammation in injured spinal cord and improve the neurological outcome. These findings can be added to the literature for reduction of inflammation in SCI and improvement of neurological outcome after transplantation of BMSCs.

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“…For instance, Piscioneri et al [47] described the development of a compartmentalized membrane system using neonatal rodent hippocampal cells and human MSCs (hMSCs) to investigate the neuroprotective effects of the latter in a oxygen-glucose deprivation (OGD) model. The goal was to simulate cerebral ischemic damage by inducing OGD for 120 min.…”

Section: Neurodegenerationmentioning

confidence: 99%

Bioengineered cell culture systems of central nervous system injury and disease

Teixeira

Vasconcelos

Gomes

et al. 2016

Drug Discovery Today

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Cell culture systems, either 2D or explant based, have been pivotal to better understand the pathophysiology of several central nervous system (CNS) disorders. Recently, bioengineered cell culture systems have been proposed as an alternative to the traditional setups. These innovative systems often combine different cell populations in 3D environments that more closely recapitulate the different niches that exist within the developing or adult CNS. Given the importance of such systems for the future of CNS-related research, we discuss here the most recent advances in the field, particularly those dealing with neurodegeneration, neurodevelopmental disorders, and trauma.

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Neuroprotective effect of human mesenchymal stem cells in a compartmentalized neuronal membrane system

Cited by 28 publications

References 36 publications

An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration

An Effective Cell Coculture Platform Based on the Electrospun Microtube Array Membrane for Nerve Regeneration

Effect of Bone Marrow-derived Mesenchymal Stem Cells on Changes of Serum Levels of TNF-α and Locomotor Function after Spinal Cord Injury in Mice

Bioengineered cell culture systems of central nervous system injury and disease

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