Bioengineered cell culture systems of central nervous system injury and disease

Abstract

Cell culture systems, either 2D or explant based, have been pivotal to better understand the pathophysiology of several central nervous system (CNS) disorders. Recently, bioengineered cell culture systems have been proposed as an alternative to the traditional setups. These innovative systems often combine different cell populations in 3D environments that more closely recapitulate the different niches that exist within the developing or adult CNS. Given the importance of such systems for the future of CNS-related research, we discuss here the most recent advances in the field, particularly those dealing with neurodegeneration, neurodevelopmental disorders, and trauma.

Pre-clinical evaluation of advanced nerve guideconduits using a novel 3D in vitro testing model

“…In 3D models, cells can be cultivated in three dimensions like in their natural environment in tissues and organs. In neurosciences, 3D models are mostly devoted to the central nervous system to study the neuronal network and signalling pathways, brain barriers, disorders and the effects of neurotoxins (reviewed by [24,25] ). Studies on peripheral nerve injuries, and nerve guides in particular, would benefit from in vitro 3D models due to the discussed limitations of animal injury models, 2D in vitro cultures and the lack of published data.…”