Neuroprotective effect of green tea extractives against oxidative stress by enhancing the survival and proliferation of PC12 cells

Cai, Zhenlu; Hu, Xiaoxuan; Tan, Ruolan; Feng, Yunran; Sun, Meiqi; Ma, Ning; Li, Xingxing; Huang, Li; An, Jing; Ge, Qian; Lü, Haixia

doi:10.1007/s13273-019-0042-8

Cited by 8 publications

(6 citation statements)

References 29 publications

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“…Following the standard protocol in our lab, 23 brain tissue sections (30 μm) from adult mice (8~12 weeks) and young mice (4 weeks) were obtained after fixation with 4% paraformaldehyde (PFA) for 24 h and dehydrated in 30% sucrose solution. NeuN and GFAP staining were performed to identify neurons and astrocytes, respectively.…”

Section: Methodsmentioning

confidence: 99%

Maternal high‐salt diet during pregnancy impairs synaptic plasticity and memory in offspring

et al. 2021

The FASEB Journal

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Sodium chloride is a major source of salt in human nutrition. It is well-known that long-term high-salt diet (HSD) was associated with the development of hypertension and cardiovascular disease, especially when sodium intake exceeds 5 g/day. 1 There is an emerging evidence that the consumptions of dietary sodium are usually excess of requirements in the form of salt in many countries among adults and childrens, and then, leading to the excess weight gain. 2 Strikingly, both experimental data from animal and epidemiological data from clinical investigations in human being demonstrated that long-term HSD may cause cognitive dysfunction and brain tissue damage in adult individuals. 3,4 Data from rodents reported that HSD impaired cognitive function without changing blood pressure. 5,6 Furthermore, HSD disturbed the short-and long-term memory of mouse with corresponding increases in hippocampal oxidative stress and deregulation of synaptic protein/neurotrophin. 6 The importance of adequate maternal nutrition during the development for offspring's long-term physical health has

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Section: Methodsmentioning

confidence: 99%

Maternal high‐salt diet during pregnancy impairs synaptic plasticity and memory in offspring

et al. 2021

The FASEB Journal

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“…Our further perspectives also deal with the structure–activity relationship studies of racemic mixtures and the design of other chromone analogues. In addition, non-mitochondrial cytoprotective mechanisms, use of other microglial cells [ 37 ], and an in vivo assessment are significant considerations to evaluate their potential as therapeutic agents against AD.…”

Section: Discussionmentioning

confidence: 99%

Diaportheone A Analogues Instigate a Neuroprotective Effect by Protecting Neuroblastoma SH-SY5Y Cells from Oxidative Stress

Tan

Захарова

2021

Biology

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Alzheimer’s disease (AD) remains an incurable neurodegenerative illness. Oxidative stress resulting in the formation of reactive oxygen species (ROS) and the abnormal deposition of amyloid-beta (Aβ) are the major pathological hallmarks associated with AD. In search for small molecules targeting multiple pathways of AD and of no known molecular targets, the neuroprotective effects of the synthetic chromones diaportheone A1 and diaportheone A2, analogues of the natural product diaportheone A, were investigated. Chromones are heterocyclic compounds bearing the benzoannelated γ-pyrone moiety and were regarded as an important class of organic molecules due to their diverse pharmacological activities. The influence of the compounds on the inhibition of Aβ aggregation was determined by Thioflavin T (ThT) assay, and the cell viability, ROS, and mitochondrial membrane potential were evaluated with human neuroblastoma SH-SY5Y cells. Results showed that both compounds inhibited the Aβ aggregation at 80.41% and 73.68% for diaportheone A1 and diaportheone A2, respectively. Increased cell viabilities were observed from the protection by both compounds using Aβ- or H2O2-induced SH-SY5Y cells. Both compounds also reduced the intracellular ROS level in Aβ- or H2O2-induced SH-SY5Y cells at 10 and 20 μM concentrations, and increased the mitochondrial membrane potentials in Aβ-induced SH-SY5Y cells at 20 μM concentration. Molecular docking experiments using the Aβ protein models 2MXU and 2BEG also indicated a good agreement with the experimental data. The results demonstrated for the first time the oxidative stress effects associated with the chromones diaportheone A1 and diaportheone A2 as potential neuroprotective therapeutic agents against AD.

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“…This polyphenol, in fact, can modulate multiple brain targets, such as many intracellular signalling pathways such as PKC, MAPK, and PI3K/Akt, survival and cell death genes (anti-apoptotic activity), can induce neurite growth, and stabilize the mitochondrial potential [ 51 ]. In particular, it has been reported that polyphenols of green tea protect PC-12 cells from H 2 O 2 -induced OS injury by enhancing cell survival and proliferation via the JNK signalling pathway [ 52 ]. Moreover, EGCG protects hippocampal neurons and neuroblastoma SH-SY5Y cells by suppressing ROS generation and by modulating the PI3K/Akt signalling cascade in order to decrease pro-apoptotic proteins such as Bax [ 48 ], in agreement with the present results.…”

Section: Discussionmentioning

confidence: 99%

Acacia catechu Willd. Extract Protects Neuronal Cells from Oxidative Stress-Induced Damage

et al. 2021

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Oxidative stress (OS) and the resulting reactive oxygen species (ROS) generation and inflammation play a pivotal role in the neuronal loss occurring during the onset of neurodegenerative diseases. Therefore, promising future drugs that would prevent or slow down the progression of neurodegeneration should possess potent radical-scavenging activity. Acacia catechu Willd. heartwood extract (AC), already characterized for its high catechin content, is endowed with antioxidant properties. The aim of the present study was to assess AC neuroprotection in both human neuroblastoma SH-SY5Y cells and rat brain slices treated with hydrogen peroxide. In SH-SY5Y cells, AC prevented a decrease in viability, as well as an increase in sub-diploid-, DAPI positive cells, reduced ROS formation, and recovered the mitochondrial potential and caspase-3 activation. AC related neuroprotective effects also occurred in rat brain slices as a reversal prevention in the expression of the main proteins involved in apoptosis and signalling pathways related to calcium homeostasis following OS-mediated injury. Additionally, unbiased quantitative mass spectrometry allowed for assessing that AC partially prevented the hydrogen peroxide-induced altered proteome, including proteins belonging to the synaptic vesicle fusion apparatus. In conclusion, the present results suggest the possibility of AC as a nutraceutical useful in preventing neurodegenerative diseases.

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Neuroprotective effect of green tea extractives against oxidative stress by enhancing the survival and proliferation of PC12 cells

Cited by 8 publications

References 29 publications

Maternal high‐salt diet during pregnancy impairs synaptic plasticity and memory in offspring

Maternal high‐salt diet during pregnancy impairs synaptic plasticity and memory in offspring

Diaportheone A Analogues Instigate a Neuroprotective Effect by Protecting Neuroblastoma SH-SY5Y Cells from Oxidative Stress

Acacia catechu Willd. Extract Protects Neuronal Cells from Oxidative Stress-Induced Damage

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