Neuroprotective effect of chlorogenic acid in global cerebral ischemia-reperfusion rat model

Kumar, Gaurav; Mukherjee, Sumedha; Pankaj, Pankaj; Singh, Sangeeta; Birla, Hareram; Singh, Surya Pratap; Krishnamurthy, Sairam; Patnaik, Ranjana

doi:10.1007/s00210-019-01670-x

Cited by 47 publications

(23 citation statements)

References 78 publications

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“…CGA significantly reduced the expression of TNF-α, iNOS and caspase-3. 36 Similarly, this study found that CGA improved CI/R-induced brain tissue pathology and cortical cell apoptosis in a dose-dependent manner by reducing oxidative stress. In addition, we also found that the role of CGA in CI/R involves the activation of Nrf2 pathway.…”

Section: Discussionsupporting

confidence: 57%

<p>Protective Effects of Chlorogenic Acid on Cerebral Ischemia/Reperfusion Injury Rats by Regulating Oxidative Stress-Related Nrf2 Pathway</p>

Liu¹,

Wang²,

Zhang³

et al. 2020

DDDT

130

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Introduction: Cerebral ischemia-reperfusion (CI/R) injury is caused by blood flow recovery after ischemic stroke. Chlorogenic acid (CGA, 5-O-caffeoylquinic acid) is a major polyphenol component of Coffea canephora, Coffea arabica L. and Mate (Ilex paraguariensis A. StHil.). Previous studies have shown that CGA has a significant neuroprotective effect and can improve global CI/R injury. However, the underlying molecular mechanism of CGA in CI/R injury has not been fully revealed. Materials: In this study, CI/R rat model was constructed. The rats were randomly divided into nine groups with ten in each group: Control, CGA (500 mg•kg-1), CI/R, CI/R + CGA (20 mg•kg-1), CI/R + CGA (100 mg•kg-1), CI/R + CGA (500 mg•kg-1), ML385 (30 mg•kg-1), CI/R + ML385 (30 mg•kg-1), CI/R + CGA + ML385. Cerebral infarction volume was detected by TTC staining. Brain pathological damage was detected by H&E staining. Apoptosis of cortical cells was detected by TUNEL staining. The expression of related proteins was detected by RT-qPCR and Western blotting. Results: Step-down test and Y maze test showed that CGA dose-dependently mitigated CI/ R-induced brain damage and enhanced learning and spatial memory. Besides, CGA promoted the expression of BDNF and NGF in a dose-dependent manner and alleviated CI/R-induced nerve injury. Moreover, CGA increased the activity of SOD and the level of GSH, as well as decreased production of ROS and LDH and the accumulation of MDA. Notably, CGA attenuated oxidative stress-induced brain injury and apoptosis and inhibited the expression of apoptosis-related proteins (cleaved caspase 3 and caspase 9). Additionally, CGA reversed CI/R induced inactivation of Nrf2 pathway and promoted Nrf2, NQO-1 and HO-1 expression. Nrf2 pathway inhibitor ML385 destroyed this promotion. Discussion: All the data indicated that CGA had a neuroprotective effect on the CI/R rats by regulating oxidative stress-related Nrf2 pathway.

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Section: Discussionsupporting

confidence: 57%

<p>Protective Effects of Chlorogenic Acid on Cerebral Ischemia/Reperfusion Injury Rats by Regulating Oxidative Stress-Related Nrf2 Pathway</p>

Liu¹,

Wang²,

Zhang³

et al. 2020

DDDT

130

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“…Some earlier long‐term follow‐up studies indicated that components of decaffeinated coffee (mostly 5‐CQA) lower the risk of NDDs such as ischemic stroke, Alzheimer's disease (AD), and Parkinson's disease (PD) (Larsson et al., 2011; Lopez‐Garcia et al., 2009; Mikami & Yamazawa, 2015; Oboh, Agunloye, Akinyemi, Ademiluyi, & Adefegha, 2013; Teraoka et al., 2012). Therefore, more and more researches focus on the relationship between 5‐CQA consumption and reduced risk of developing NDDs, as well as the potential mechanisms of neuroprotection (Heitman & Ingram, 2017; Kumar, Mukherjee, et al., 2019; Nabavi et al., 2017).…”

Section: Beneficial Properties For Healthmentioning

confidence: 99%

Chlorogenic acid: A comprehensive review of the dietary sources, processing effects, bioavailability, beneficial properties, mechanisms of action, and future directions

Tian

Cui

et al. 2020

Comp Rev Food Sci Food Safe

199

179

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Chlorogenic acids (CGAs), a group of hydroxycinnamates, are generally abundant in everyday foods and beverages, most prominently in certain coffee drinks. Among them, the chlorogenic acid (CGA), also termed as 5-O-caffeoylquinic acid (5-CQA), is one of the most abundant, highly functional polyphenolic compounds in the human diet. The evidence of its health benefits obtained from clinical studies, as well as basic research, indicates an inverse correlation between 5-CQA consumption and a lower risk of metabolic syndromes and chronic diseases. This review focuses on the beneficial properties for health and mechanisms of action of 5-CQA, starting with its history, isomers, dietary sources, processing effects, preparation methods, pharmacological safety evaluation, and bioavailability. It also provides the possible molecular mechanistic bases to explain the health beneficial effects of 5-CQA including neuroprotective, cardiovascular protective, gastrointestinal protective, renoprotective, hepatoprotective, glucose and lipid metabolism regulatory, and anticarcinogenic effects. The information summarized here could aid in the basic and clinical research on 5-CQA as a natural dietary additive, potential drug candidate, as well as a natural health promoter.

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“…Chlorogenic acid (CGA), an ester formed from caffeic acid and L-quinic acid, is the major polyphenolic compound in coffee isolated from the leaves and fruits of dicotyledonous plants including Coffea canephora and Coffea arabica L. [ 12 ]. It has been reported that pretreatment with CGA attenuates neuronal loss in the cerebral cortex following transient focal brain ischemia induced by middle cerebral artery occlusion in rats [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Experimental Pretreatment with Chlorogenic Acid Prevents Transient Ischemia-Induced Cognitive Decline and Neuronal Damage in the Hippocampus through Anti-Oxidative and Anti-Inflammatory Effects

et al. 2020

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Chlorogenic acid (CGA), an ester of caffeic acid and quinic acid, is among the phenolic acid compounds which can be naturally found in green coffee extract and tea. CGA has been studied since it displays significant pharmacological properties. The aim of this study was to investigate the effects of CGA on cognitive function and neuroprotection including its mechanisms in the hippocampus following transient forebrain ischemia in gerbils. Memory and learning following the ischemia was investigated by eight-arm radial maze and passive avoidance tests. Neuroprotection was examined by immunohistochemistry for neuronal nuclei-specific protein and Fluoro-Jade B histofluorescence staining. For mechanisms of the neuroprotection, alterations in copper, zinc-superoxide dismutase (SOD1), SOD2 as antioxidant enzymes, dihydroethidium and 4-hydroxy-2-nonenal as indicators for oxidative stress, and anti-inflammatory cytokines (interleukin (IL)-4 and IL-13) and pro-inflammatory cytokines (tumor necrosis factor α (TNF-α) and IL-2) were examined by Western blotting and/or immunohistochemistry. As a result, pretreatment with 30 mg/kg CGA attenuated cognitive impairment and displayed a neuroprotective effect against transient forebrain ischemia (TFI). In Western blotting, the expression levels of SOD2 and IL-4 were increased due to pretreatment with CGA and, furthermore, 4-HNE production and IL-4 expressions were inhibited by CGA pretreatment. Additionally, pretreated CGA enhanced antioxidant enzymes and anti-inflammatory cytokines and, in contrast, attenuated oxidative stress and pro-inflammatory cytokine expression. Based on these results, we suggest that CGA can be a useful neuroprotective material against ischemia-reperfusion injury due to its antioxidant and anti-inflammatory efficacies.

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Neuroprotective effect of chlorogenic acid in global cerebral ischemia-reperfusion rat model

Cited by 47 publications

References 78 publications

<p>Protective Effects of Chlorogenic Acid on Cerebral Ischemia/Reperfusion Injury Rats by Regulating Oxidative Stress-Related Nrf2 Pathway</p>

<p>Protective Effects of Chlorogenic Acid on Cerebral Ischemia/Reperfusion Injury Rats by Regulating Oxidative Stress-Related Nrf2 Pathway</p>

Chlorogenic acid: A comprehensive review of the dietary sources, processing effects, bioavailability, beneficial properties, mechanisms of action, and future directions

Experimental Pretreatment with Chlorogenic Acid Prevents Transient Ischemia-Induced Cognitive Decline and Neuronal Damage in the Hippocampus through Anti-Oxidative and Anti-Inflammatory Effects

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