Neuroprotective actions of leptin facilitated through balancing mitochondrial morphology and improving mitochondrial function

Cheng, Ying; Buchan, Matthew J.; Vitanova, Karina S.; Aitken, Laura; Gunn‐Moore, Frank; Ramsay, Rona R.; Doherty, Gayle H.

doi:10.1111/jnc.15003

Cited by 16 publications

(11 citation statements)

References 84 publications

(113 reference statements)

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“…MAO A levels are influenced by both genetic and environmental factors, and MAO A activity influences psychopathologies [136,137]. The anorexigenic hormone leptin that protects mitochondrial function regulates the expression and activity of MAO B [138]. Leptin also reduced the increase in MAO A and MAO B expression found in hippocampal neurons treated with the Alzheimer's disease peptide Aβ.…”

Section: Does Mao a Moonliaght In The Cell?mentioning

confidence: 99%

Questions in the Chemical Enzymology of MAO

Ramsay

Albreht

2021

Chemistry

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We have structure, a wealth of kinetic data, thousands of chemical ligands and clinical information for the effects of a range of drugs on monoamine oxidase activity in vivo. We have comparative information from various species and mutations on kinetics and effects of inhibition. Nevertheless, there are what seem like simple questions still to be answered. This article presents a brief summary of existing experimental evidence the background and poses questions that remain intriguing for chemists and biochemists researching the chemical enzymology of and drug design for monoamine oxidases (FAD-containing EC 4.1.3.4).

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Section: Does Mao a Moonliaght In The Cell?mentioning

confidence: 99%

Questions in the Chemical Enzymology of MAO

Ramsay

Albreht

2021

Chemistry

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“…Human neuroblastoma cell lines, such as SH-SY5Y, are a widely used in vitro model system, and have been utilised for a plethora of neuroscience applications including cell viability assays ( Jaworska-Feil et al, 2010 , Funakohi-Tago et al, 2018 ), neuronal ultrastructure ( Cheng et al, 2020 ), studies of neurophysiology ( Tosetti et al, 1998 ; Öz and Çelik, 2016 ) and testing of pharmacological preparations ( Cheung et al, 2009 , Jiang et al, 2018 ). These cells were originally derived from the SK-N-SH cell line which was sub-cloned three times to SH-SY, SH-SY5 and finally to the SH-SY5Y cell line.…”

Section: Introductionmentioning

confidence: 99%

Long-term culture of SH-SY5Y neuroblastoma cells in the absence of neurotrophins: A novel model of neuronal ageing

Strother

Miles

Holiday

et al. 2021

Journal of Neuroscience Methods

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Background Studying human ageing is of increasing importance due to the worldwide ageing population. However, it faces the challenge of lengthy experiments to produce an ageing phenotype. Often, to recreate the hallmarks of ageing requires complex empirical conditions that can confound data interpretation. Indeed, many studies use whole organisms with relatively short life spans, which may have little, or limited, relevance to human ageing. There has been extensive use of cell lines to study ageing in human somatic cells, but the modelling of human neuronal ageing is somewhat more complex in vitro . New Method We cultured the well-characterised SH-SY5Y human neural cell line to produce high purity cultures of cells differentiated to express a neuronal phenotype, and designed a protocol to maintain these cells in culture until they accumulated biomarkers of cellular ageing. Results Our data validate a novel and simple technique for the efficient differentiation and long-term maintenance of SH-SY5Y cells, expressing markers of neuronal differentiation and demonstrating electrical activity in culture. Over time in vitro , these cells progressively accumulate markers of ageing such as enhanced production of reactive oxygen species and accumulation of oxidative damage. Comparison to Existing Methods In comparison to existing techniques to model neuronal ageing our method is cost effective, requiring no specialist equipment or growth factors. Conclusions We demonstrate that SH-SY5Y cells, grown under these culture conditions, represent a simple model of neuronal ageing that is amenable to cell biological, biochemical and electrophysiological investigation.

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“…Taken together, our data suggest the anti-inflammation potential of SERPINA3C which may contribute to its protective effects against obesity and metabolic disorders. Studies have established a consensus that inflammatory mediators impaired mitochondrial metabolism, and that defective mitochondrial function led to decreased energy expenditure, which contributes to the initiation and progression of obesity [ [32] , [33] , [34] ]. Based on our data, positive correlation between SERPINA3C expression level and energy expenditure, and negative correlation between SERPINA3C expression level and obesity were observed.…”

Section: Resultsmentioning

confidence: 99%

SERPINA3C ameliorates adipose tissue inflammation through the Cathepsin G/Integrin/AKT pathway

Guo

Xiao

et al. 2022

Molecular Metabolism

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Neuroprotective actions of leptin facilitated through balancing mitochondrial morphology and improving mitochondrial function

Abstract: This is an open access article under the terms of the Creat ive Commo ns Attri bution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

Cited by 16 publications

References 84 publications

Questions in the Chemical Enzymology of MAO

Questions in the Chemical Enzymology of MAO

Long-term culture of SH-SY5Y neuroblastoma cells in the absence of neurotrophins: A novel model of neuronal ageing

SERPINA3C ameliorates adipose tissue inflammation through the Cathepsin G/Integrin/AKT pathway

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